ID Q740L5_MYCPA Unreviewed; 722 AA.
AC Q740L5;
DT 05-JUL-2004, integrated into UniProtKB/TrEMBL.
DT 05-JUL-2004, sequence version 1.
DT 01-MAY-2013, entry version 70.
DE RecName: Full=UvrABC system protein B;
DE Short=Protein UvrB;
DE AltName: Full=Excinuclease ABC subunit B;
GN Name=uvrB; OrderedLocusNames=MAP_1335;
OS Mycobacterium paratuberculosis (strain ATCC BAA-968 / K-10).
OC Bacteria; Actinobacteria; Actinobacteridae; Actinomycetales;
OC Corynebacterineae; Mycobacteriaceae; Mycobacterium;
OC Mycobacterium avium complex (MAC).
OX NCBI_TaxID=262316;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC BAA-968 / K-10;
RX PubMed=16116077; DOI=10.1073/pnas.0505662102;
RA Li L., Bannantine J.P., Zhang Q., Amonsin A., May B.J., Alt D.,
RA Banerji N., Kanjilal S., Kapur V.;
RT "The complete genome sequence of Mycobacterium avium subspecies
RT paratuberculosis.";
RL Proc. Natl. Acad. Sci. U.S.A. 102:12344-12349(2005).
CC -!- FUNCTION: The UvrABC repair system catalyzes the recognition and
CC processing of DNA lesions. A damage recognition complex composed
CC of 2 UvrA and 2 UvrB subunits scans DNA for abnormalities. Upon
CC binding of the UvrA(2)B(2) complex to a putative damaged site, the
CC DNA wraps around one UvrB monomer. DNA wrap is dependent on ATP
CC binding by UvrB and probably causes local melting of the DNA
CC helix, facilitating insertion of UvrB beta-hairpin between the DNA
CC strands. Then UvrB probes one DNA strand for the presence of a
CC lesion. If a lesion is found the UvrA subunits dissociate and the
CC UvrB-DNA preincision complex is formed. This complex is
CC subsequently bound by UvrC and the second UvrB is released. If no
CC lesion is found, the DNA wraps around the other UvrB subunit that
CC will check the other stand for damage (By similarity).
CC -!- SUBUNIT: Forms a heterotetramer with UvrA during the search for
CC lesions. Interacts with UvrC in an incision complex (By
CC similarity).
CC -!- SUBCELLULAR LOCATION: Cytoplasm (By similarity).
CC -!- DOMAIN: The beta-hairpin motif is involved in DNA binding (By
CC similarity).
CC -!- SIMILARITY: Belongs to the UvrB family.
CC -!- SIMILARITY: Contains 1 UVR domain.
CC -!- SIMILARITY: Contains 1 helicase ATP-binding domain.
CC -!- SIMILARITY: Contains 1 helicase C-terminal domain.
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DR EMBL; AE016958; AAS03652.1; -; Genomic_DNA.
DR RefSeq; NP_960269.1; NC_002944.2.
DR ProteinModelPortal; Q740L5; -.
DR STRING; 262316.MAP1335; -.
DR EnsemblBacteria; AAS03652; AAS03652; MAP_1335.
DR GeneID; 2720724; -.
DR KEGG; mpa:MAP1335; -.
DR PATRIC; 17995157; VBIMycAvi108102_1408.
DR KO; K03702; -.
DR OMA; CIYGLGI; -.
DR ProtClustDB; PRK05298; -.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:HAMAP.
DR GO; GO:0003677; F:DNA binding; IEA:HAMAP.
DR GO; GO:0009381; F:excinuclease ABC activity; IEA:HAMAP.
DR GO; GO:0004386; F:helicase activity; IEA:HAMAP.
DR GO; GO:0090305; P:nucleic acid phosphodiester bond hydrolysis; IEA:GOC.
DR GO; GO:0006289; P:nucleotide-excision repair; IEA:HAMAP.
DR GO; GO:0009432; P:SOS response; IEA:HAMAP.
DR Gene3D; 4.10.860.10; -; 1.
DR HAMAP; MF_00204; UvrB; 1; -.
DR InterPro; IPR006935; Helicase/UvrB_dom.
DR InterPro; IPR014001; Helicase_ATP-bd.
DR InterPro; IPR001650; Helicase_C.
DR InterPro; IPR001943; UVR_dom.
DR InterPro; IPR004807; UvrB.
DR InterPro; IPR024759; UvrB_YAD/RRR_dom.
DR PANTHER; PTHR24029; PTHR24029; 1.
DR Pfam; PF00271; Helicase_C; 1.
DR Pfam; PF04851; ResIII; 1.
DR Pfam; PF02151; UVR; 1.
DR Pfam; PF12344; UvrB; 1.
DR SMART; SM00487; DEXDc; 1.
DR SMART; SM00490; HELICc; 1.
DR SUPFAM; SSF46600; UvrB_C; 1.
DR TIGRFAMs; TIGR00631; uvrb; 1.
DR PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR PROSITE; PS51194; HELICASE_CTER; 1.
DR PROSITE; PS50151; UVR; 1.
PE 3: Inferred from homology;
KW ATP-binding; Complete proteome; Cytoplasm; DNA damage; DNA excision;
KW DNA repair; Excision nuclease; Helicase; Hydrolase;
KW Nucleotide-binding; SOS response.
FT DOMAIN 53 213 Helicase ATP-binding (By similarity).
FT DOMAIN 457 623 Helicase C-terminal (By similarity).
FT DOMAIN 677 712 UVR (By similarity).
FT NP_BIND 66 73 ATP (By similarity).
FT MOTIF 119 142 Beta-hairpin (By similarity).
SQ SEQUENCE 722 AA; 80921 MW; 208339B4571069BC CRC64;
MAFATEHPVV AHSEYRPAEE AVEGLVRAGG RFEVVSPHAP AGDQPAAIDE LERRIRAGER
DVVLLGATGT GKSATTAWLI ERLQRPTLVM APNKTLAAQL ANELREMLPH NAVEYFVSYY
DYYQPEAYIA QTDTYIEKDS SINDDVERLR HSATSSLLSR RDVVVVASVS CIYGLGTPQS
YLDRSVELRV GTEVPRDALL RLLVDVQYTR NDLSFTRGSF RVRGDTVEII PSYEELAVRI
EFFGDEIEAL YYLHPLTGDV IRQVDSLRIF PATHYVAGPE RMAHAISTIE QELAERLAEL
EGQGKLLEAQ RLRMRTNYDI EMMRQVGFCS GIENYSRHID GRGPGSPPAT LLDYFPEDFL
LVIDESHVTV PQIGGMYEGD MSRKRNLVEY GFRLPSACDN RPLTWEEFAD RIGQTVYLSA
TPGPYELSQT GGEFVEQVIR PTGLVDPKVV VKPTKGQIDD LIGEIRKRTE ADERVLVTTL
TKKMAEDLTD YLLEMGIRVR YLHSEVDTLR RVELLRQLRL GEYDVLVGIN LLREGLDLPE
VSLVAILDAD KEGFLRSARS LIQTIGRAAR NVSGEVHMYA DTITESMKEA IDETERRRAK
QIAYNEAHGI DPQPLRKKIA DILDQVYREA DDTDTVEIGS GRSMSRGRRA QGEPGRAVSA
GIVEGRDTTN MPRAELADLI KDLTAQMMAA ARDLQFELAA RFRDEIADLK KELRGMDAAG
LK
//
