ID Q803Z0_DANRE Unreviewed; 577 AA.
AC Q803Z0;
DT 01-JUN-2003, integrated into UniProtKB/TrEMBL.
DT 01-JUN-2003, sequence version 1.
DT 27-MAR-2024, entry version 142.
DE RecName: Full=non-specific serine/threonine protein kinase {ECO:0000256|ARBA:ARBA00012513};
DE EC=2.7.11.1 {ECO:0000256|ARBA:ARBA00012513};
GN Name=pak1 {ECO:0000313|EMBL:AAH44138.1,
GN ECO:0000313|RefSeq:NP_958485.1, ECO:0000313|ZFIN:ZDB-GENE-030826-29};
GN Synonyms=cb761 {ECO:0000313|RefSeq:NP_958485.1};
OS Danio rerio (Zebrafish) (Brachydanio rerio).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC Danionidae; Danioninae; Danio.
OX NCBI_TaxID=7955 {ECO:0000313|EMBL:AAH44138.1};
RN [1] {ECO:0000313|EMBL:AAH44138.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=AB {ECO:0000313|EMBL:AAH44138.1};
RC TISSUE=PCR rescue {ECO:0000313|EMBL:AAI65171.1}, and Whole body
RC {ECO:0000313|EMBL:AAH44138.1};
RG NIH - Zebrafish Gene Collection (ZGC) project;
RL Submitted (JAN-2003) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|RefSeq:NP_958485.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=AB {ECO:0000313|RefSeq:NP_958485.1};
RX PubMed=16603149;
RA Pujic Z., Omori Y., Tsujikawa M., Thisse B., Thisse C., Malicki J.;
RT "Reverse genetic analysis of neurogenesis in the zebrafish retina.";
RL Dev. Biol. 293:330-347(2006).
RN [3] {ECO:0000313|RefSeq:NP_958485.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=AB {ECO:0000313|RefSeq:NP_958485.1};
RX PubMed=17949705;
RA Veldman M.B., Bemben M.A., Thompson R.C., Goldman D.;
RT "Gene expression analysis of zebrafish retinal ganglion cells during optic
RT nerve regeneration identifies KLF6a and KLF7a as important regulators of
RT axon regeneration.";
RL Dev. Biol. 312:596-612(2007).
RN [4] {ECO:0000313|RefSeq:NP_958485.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=AB {ECO:0000313|RefSeq:NP_958485.1};
RX PubMed=17573532; DOI=10.1073/pnas.0700825104;
RA Liu J., Fraser S.D., Faloon P.W., Rollins E.L., Vom Berg J.,
RA Starovic-Subota O., Laliberte A.L., Chen J.N., Serluca F.C., Childs S.J.;
RT "A betaPix Pak2a signaling pathway regulates cerebral vascular stability in
RT zebrafish.";
RL Proc. Natl. Acad. Sci. U.S.A. 104:13990-13995(2007).
RN [5] {ECO:0000313|RefSeq:NP_958485.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=AB {ECO:0000313|RefSeq:NP_958485.1};
RX PubMed=19557173;
RA Lightcap C.M., Kari G., Arias-Romero L.E., Chernoff J., Rodeck U.,
RA Williams J.C.;
RT "Interaction with LC8 is required for Pak1 nuclear import and is
RT indispensable for zebrafish development.";
RL PLoS ONE 4:E6025-E6025(2009).
RN [6] {ECO:0000313|RefSeq:NP_958485.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=AB {ECO:0000313|RefSeq:NP_958485.1};
RX PubMed=20405038;
RA Tay H.G., Ng Y.W., Manser E.;
RT "A vertebrate-specific Chp-PAK-PIX pathway maintains E-cadherin at adherens
RT junctions during zebrafish epiboly.";
RL PLoS ONE 5:e10125-e10125(2010).
RN [7] {ECO:0000313|Proteomes:UP000000437}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=23594743; DOI=10.1038/nature12111;
RG Genome Reference Consortium Zebrafish;
RA Howe K., Clark M.D., Torroja C.F., Torrance J., Berthelot C., Muffato M.,
RA Collins J.E., Humphray S., McLaren K., Matthews L., McLaren S., Sealy I.,
RA Caccamo M., Churcher C., Scott C., Barrett J.C., Koch R., Rauch G.J.,
RA White S., Chow W., Kilian B., Quintais L.T., Guerra-Assuncao J.A., Zhou Y.,
RA Gu Y., Yen J., Vogel J.H., Eyre T., Redmond S., Banerjee R., Chi J., Fu B.,
RA Langley E., Maguire S.F., Laird G.K., Lloyd D., Kenyon E., Donaldson S.,
RA Sehra H., Almeida-King J., Loveland J., Trevanion S., Jones M., Quail M.,
RA Willey D., Hunt A., Burton J., Sims S., McLay K., Plumb B., Davis J.,
RA Clee C., Oliver K., Clark R., Riddle C., Elliot D., Eliott D.,
RA Threadgold G., Harden G., Ware D., Begum S., Mortimore B., Mortimer B.,
RA Kerry G., Heath P., Phillimore B., Tracey A., Corby N., Dunn M.,
RA Johnson C., Wood J., Clark S., Pelan S., Griffiths G., Smith M.,
RA Glithero R., Howden P., Barker N., Lloyd C., Stevens C., Harley J.,
RA Holt K., Panagiotidis G., Lovell J., Beasley H., Henderson C., Gordon D.,
RA Auger K., Wright D., Collins J., Raisen C., Dyer L., Leung K.,
RA Robertson L., Ambridge K., Leongamornlert D., McGuire S., Gilderthorp R.,
RA Griffiths C., Manthravadi D., Nichol S., Barker G., Whitehead S., Kay M.,
RA Brown J., Murnane C., Gray E., Humphries M., Sycamore N., Barker D.,
RA Saunders D., Wallis J., Babbage A., Hammond S., Mashreghi-Mohammadi M.,
RA Barr L., Martin S., Wray P., Ellington A., Matthews N., Ellwood M.,
RA Woodmansey R., Clark G., Cooper J., Cooper J., Tromans A., Grafham D.,
RA Skuce C., Pandian R., Andrews R., Harrison E., Kimberley A., Garnett J.,
RA Fosker N., Hall R., Garner P., Kelly D., Bird C., Palmer S., Gehring I.,
RA Berger A., Dooley C.M., Ersan-Urun Z., Eser C., Geiger H., Geisler M.,
RA Karotki L., Kirn A., Konantz J., Konantz M., Oberlander M.,
RA Rudolph-Geiger S., Teucke M., Lanz C., Raddatz G., Osoegawa K., Zhu B.,
RA Rapp A., Widaa S., Langford C., Yang F., Schuster S.C., Carter N.P.,
RA Harrow J., Ning Z., Herrero J., Searle S.M., Enright A., Geisler R.,
RA Plasterk R.H., Lee C., Westerfield M., de Jong P.J., Zon L.I.,
RA Postlethwait J.H., Nusslein-Volhard C., Hubbard T.J., Roest Crollius H.,
RA Rogers J., Stemple D.L.;
RT "The zebrafish reference genome sequence and its relationship to the human
RT genome.";
RL Nature 496:498-503(2013).
RN [8] {ECO:0000313|RefSeq:NP_958485.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=AB {ECO:0000313|RefSeq:NP_958485.1};
RX PubMed=24823378;
RA Kelly M.L., Astsaturov A., Rhodes J., Chernoff J.;
RT "A Pak1/Erk signaling module acts through Gata6 to regulate cardiovascular
RT development in zebrafish.";
RL Dev. Cell 29:350-359(2014).
RN [9] {ECO:0000313|RefSeq:NP_958485.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=AB {ECO:0000313|RefSeq:NP_958485.1};
RX PubMed=27189481;
RA Pasquier J., Cabau C., Nguyen T., Jouanno E., Severac D., Braasch I.,
RA Journot L., Pontarotti P., Klopp C., Postlethwait J.H., Guiguen Y.,
RA Bobe J.;
RT "Gene evolution and gene expression after whole genome duplication in fish:
RT the PhyloFish database.";
RL BMC Genomics 17:368-368(2016).
RN [10] {ECO:0000313|RefSeq:NP_958485.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=AB {ECO:0000313|RefSeq:NP_958485.1};
RX PubMed=27581547;
RA Jagadeeshan S., Sagayaraj R.V., Paneerselvan N., Ghouse S.S., Malathi R.;
RT "Toxicity and anti-angiogenicity evaluation of Pak1 inhibitor IPA-3 using
RT zebrafish embryo model.";
RL Cell Biol. Toxicol. 33:41-56(2017).
RN [11] {ECO:0000313|RefSeq:NP_958485.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=AB {ECO:0000313|RefSeq:NP_958485.1};
RX PubMed=27910850;
RA Wei S., Dai M., Liu Z., Ma Y., Shang H., Cao Y., Wang Q.;
RT "The guanine nucleotide exchange factor Net1 facilitates the specification
RT of dorsal cell fates in zebrafish embryos by promoting maternal beta-
RT catenin activation.";
RL Cell Res. 27:202-225(2017).
RN [12] {ECO:0000313|RefSeq:NP_958485.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=AB {ECO:0000313|RefSeq:NP_958485.1};
RX PubMed=28211472;
RA Fukuda R., Gunawan F., Beisaw A., Jimenez-Amilburu V., Maischein H.M.,
RA Kostin S., Kawakami K., Stainier D.Y.;
RT "Proteolysis regulates cardiomyocyte maturation and tissue integration.";
RL Nat. Commun. 8:14495-14495(2017).
RN [13] {ECO:0000313|RefSeq:NP_958485.1}
RP IDENTIFICATION.
RC STRAIN=AB {ECO:0000313|RefSeq:NP_958485.1};
RG RefSeq;
RL Submitted (NOV-2023) to UniProtKB.
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DR EMBL; BC044138; AAH44138.1; -; mRNA.
DR EMBL; BC165171; AAI65171.1; -; mRNA.
DR RefSeq; NP_958485.1; NM_201328.1.
DR GeneID; 373103; -.
DR KEGG; dre:373103; -.
DR AGR; ZFIN:ZDB-GENE-030826-29; -.
DR CTD; 5058; -.
DR ZFIN; ZDB-GENE-030826-29; pak1.
DR OrthoDB; 460351at2759; -.
DR Proteomes; UP000000437; Chromosome 18.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0042802; F:identical protein binding; IPI:ZFIN.
DR GO; GO:0004672; F:protein kinase activity; IMP:ZFIN.
DR GO; GO:0004674; F:protein serine/threonine kinase activity; IBA:GO_Central.
DR GO; GO:0003232; P:bulbus arteriosus development; IMP:ZFIN.
DR GO; GO:0060070; P:canonical Wnt signaling pathway; IMP:ZFIN.
DR GO; GO:0060026; P:convergent extension; IMP:ZFIN.
DR GO; GO:0048703; P:embryonic viscerocranium morphogenesis; IMP:ZFIN.
DR GO; GO:0007507; P:heart development; IMP:ZFIN.
DR GO; GO:0001947; P:heart looping; IMP:ZFIN.
DR GO; GO:0035556; P:intracellular signal transduction; IBA:GO_Central.
DR GO; GO:0001755; P:neural crest cell migration; IMP:ZFIN.
DR GO; GO:0016310; P:phosphorylation; IEA:UniProtKB-KW.
DR GO; GO:0002572; P:pro-T cell differentiation; IMP:ZFIN.
DR GO; GO:0032956; P:regulation of actin cytoskeleton organization; IBA:GO_Central.
DR GO; GO:0050770; P:regulation of axonogenesis; IBA:GO_Central.
DR GO; GO:0043408; P:regulation of MAPK cascade; IBA:GO_Central.
DR GO; GO:0044332; P:Wnt signaling pathway involved in dorsal/ventral axis specification; IMP:ZFIN.
DR CDD; cd01093; CRIB_PAK_like; 1.
DR Gene3D; 3.90.810.10; CRIB domain; 1.
DR Gene3D; 1.10.510.10; Transferase(Phosphotransferase) domain 1; 1.
DR InterPro; IPR000095; CRIB_dom.
DR InterPro; IPR036936; CRIB_dom_sf.
DR InterPro; IPR011009; Kinase-like_dom_sf.
DR InterPro; IPR033923; PAK_BD.
DR InterPro; IPR000719; Prot_kinase_dom.
DR InterPro; IPR017441; Protein_kinase_ATP_BS.
DR InterPro; IPR008271; Ser/Thr_kinase_AS.
DR PANTHER; PTHR48015; SERINE/THREONINE-PROTEIN KINASE TAO; 1.
DR PANTHER; PTHR48015:SF20; SERINE_THREONINE-PROTEIN KINASE PAK 1; 1.
DR Pfam; PF00786; PBD; 1.
DR Pfam; PF00069; Pkinase; 1.
DR SMART; SM00285; PBD; 1.
DR SMART; SM00220; S_TKc; 1.
DR SUPFAM; SSF56112; Protein kinase-like (PK-like); 1.
DR PROSITE; PS50108; CRIB; 1.
DR PROSITE; PS00107; PROTEIN_KINASE_ATP; 1.
DR PROSITE; PS50011; PROTEIN_KINASE_DOM; 1.
DR PROSITE; PS00108; PROTEIN_KINASE_ST; 1.
PE 1: Evidence at protein level;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|PROSITE-
KW ProRule:PRU10141};
KW Kinase {ECO:0000313|EMBL:AAH44138.1, ECO:0000313|RefSeq:NP_958485.1};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741, ECO:0000256|PROSITE-
KW ProRule:PRU10141};
KW Proteomics identification {ECO:0007829|PeptideAtlas:Q803Z0};
KW Reference proteome {ECO:0000313|Proteomes:UP000000437};
KW Transferase {ECO:0000313|EMBL:AAH44138.1, ECO:0000313|RefSeq:NP_958485.1}.
FT DOMAIN 74..87
FT /note="CRIB"
FT /evidence="ECO:0000259|PROSITE:PS50108"
FT DOMAIN 302..553
FT /note="Protein kinase"
FT /evidence="ECO:0000259|PROSITE:PS50011"
FT REGION 1..72
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 158..283
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 18..32
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 216..271
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 331
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU10141"
SQ SEQUENCE 577 AA; 63261 MW; F4D62B27E620FB10 CRC64;
MSDNGEVEDK PPAPPMRNTS TMIGSSGKDS GPLNHGSKPL PPNPEDKRRK EGFIRSILTG
GGDKTNKKKE RPEISLPSDF EHTIHVGFDA VTGEFTGMPE QWARLLQTSN ITKLEQKKNP
QAVLDVLKFY DSKETSNNQK YMSFTDKGVE NFNSSSAVVP KTVSETPAVA TVSEDEDEEE
DEATPPPVIA PRPENTKSIY TRSVIEPLPP PLSTKDAATS PITNAAADGG SSSNAPSSNT
NTTNTTNTTN TTNTTSTSAA SSTNAPSTPQ TTDKSRKKKM SDEEILEKLR SIVSVGDPKK
KYTRFEKIGQ GASGTVYTAI DIATGQEVAI KQMNLQQQPK KELIINEILV MRENKNPNIV
NYLDSYLVGD ELWVVMEYLA GGSLTDVVTE TCMDEAQIAA VCRECLQALE FLHCNQVIHR
DIKSDNILLG MDGSVKLTDF GFCAQITPEQ SKRSTMVGTP YWMAPEVVTR KAYGPKVDIW
SLGIMAIEMI EGEPPYLNEN PLRALYLIAT NGTPELQNPE KLSAVFRDFL NRCLEMDVEK
RGSAKELLQH QFLKVAKPLS SLTPLIIAAK EAAKNNR
//
