ID R4S123_PHYAS Unreviewed; 713 AA.
AC R4S123;
DT 24-JUL-2013, integrated into UniProtKB/TrEMBL.
DT 24-JUL-2013, sequence version 1.
DT 27-MAR-2024, entry version 50.
DE RecName: Full=DNA 3'-5' helicase {ECO:0000256|ARBA:ARBA00034808};
DE EC=5.6.2.4 {ECO:0000256|ARBA:ARBA00034808};
GN Name=pcrA {ECO:0000313|EMBL:AGL90438.1};
GN ORFNames=SLY_0519 {ECO:0000313|EMBL:AGL90438.1};
OS Strawberry lethal yellows phytoplasma (CPA) str. NZSb11.
OC Bacteria; Mycoplasmatota; Mollicutes; Acholeplasmatales;
OC Acholeplasmataceae; Phytoplasma; 16SrXII (Stolbur group).
OX NCBI_TaxID=980422 {ECO:0000313|EMBL:AGL90438.1, ECO:0000313|Proteomes:UP000013941};
RN [1] {ECO:0000313|EMBL:AGL90438.1, ECO:0000313|Proteomes:UP000013941}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=NZSb11 {ECO:0000313|EMBL:AGL90438.1,
RC ECO:0000313|Proteomes:UP000013941};
RX PubMed=23915186; DOI=10.1186/1471-2164-14-529;
RA Andersen M.T., Liefting L.W., Havukkala I., Beever R.E.;
RT "Comparison of the complete genome sequence of two closely related isolates
RT of 'Candidatus Phytoplasma australiense' reveals genome plasticity.";
RL BMC Genomics 14:529-529(2013).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=5.6.2.4;
CC Evidence={ECO:0000256|ARBA:ARBA00034618};
CC -!- SIMILARITY: Belongs to the helicase family. UvrD subfamily.
CC {ECO:0000256|ARBA:ARBA00009922}.
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DR EMBL; CP002548; AGL90438.1; -; Genomic_DNA.
DR RefSeq; WP_015637985.1; NC_021236.1.
DR AlphaFoldDB; R4S123; -.
DR KEGG; nzs:SLY_0519; -.
DR PATRIC; fig|980422.3.peg.476; -.
DR HOGENOM; CLU_004585_5_2_14; -.
DR OrthoDB; 9810135at2; -.
DR Proteomes; UP000013941; Chromosome.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0003678; F:DNA helicase activity; IEA:InterPro.
DR CDD; cd17932; DEXQc_UvrD; 1.
DR Gene3D; 1.10.10.160; -; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR InterPro; IPR013986; DExx_box_DNA_helicase_dom_sf.
DR InterPro; IPR014017; DNA_helicase_UvrD-like_C.
DR InterPro; IPR000212; DNA_helicase_UvrD/REP.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR014016; UvrD-like_ATP-bd.
DR PANTHER; PTHR11070:SF48; ATP-DEPENDENT HELICASE_NUCLEASE SUBUNIT A; 1.
DR PANTHER; PTHR11070; UVRD / RECB / PCRA DNA HELICASE FAMILY MEMBER; 1.
DR Pfam; PF00580; UvrD-helicase; 1.
DR Pfam; PF13361; UvrD_C; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR PROSITE; PS51198; UVRD_HELICASE_ATP_BIND; 1.
DR PROSITE; PS51217; UVRD_HELICASE_CTER; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|PROSITE-
KW ProRule:PRU00560};
KW Helicase {ECO:0000256|ARBA:ARBA00022806, ECO:0000256|PROSITE-
KW ProRule:PRU00560};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|PROSITE-
KW ProRule:PRU00560};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741, ECO:0000256|PROSITE-
KW ProRule:PRU00560}.
FT DOMAIN 10..291
FT /note="UvrD-like helicase ATP-binding"
FT /evidence="ECO:0000259|PROSITE:PS51198"
FT DOMAIN 292..583
FT /note="UvrD-like helicase C-terminal"
FT /evidence="ECO:0000259|PROSITE:PS51217"
FT BINDING 31..38
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00560"
SQ SEQUENCE 713 AA; 83682 MW; 354E51BEAF98FB4E CRC64;
MSSKIDSWLK KLNKNQVEAA TSKSLATYLL AGAGTGKTTT LTTKIAYLIE KQNFLSKNIL
TLTFTNKAAD QMKNKILEMI GEKSKGVNIL TFHALGNRIL RQFINILPFN YNRNFIILND
KECNQILKIV AKEVNFDIQK VENIDNLRKE VFRLKKKTNE FLQHQQTSTQ NNNPLIVKIK
NQYQKYLQKN NLVDFNDLIL YTYQLLKEYP EIKQFWQKKF QYILVDEFQD TDFYQYQILT
FLTEKNPCIF VVGDPDQNIY SFRGACFGNS QLFLNKFNPQ IYVLKQNYRS SENILEKANL
LISYNKDHPF KKKLNSVKGK GTKVNAHFFV DSRIEAQYIV QKIKHLVQIY HYNYRDFAIL
YRVNSLGHFL EKYLISAGIP YMFLGNLSFY QKKVVKDFLA YLHLAVNPLQ DFYLRRVINV
PKRQIGKITI TCLEKIVQEK ERSFFEILKD YKDIPVSDKT QKQLYQFYCL IEELNNKIIN
NEFEKLSDII TYVDQKTQYS YQISLETQKN PQKSNEEQSS LVAIEELKSI LNQIDESFQG
SNLQKINNFL EEVSLNNETK INNSSGNKAN NRVKLATIHK VKGLEFKVVF LAGLEKEFFD
DLDDIQESRR LTYVAVTRAQ EKLFLTGVSQ RMLFGKNFSH EPSVFFKEMG FGSRKNYFLS
QTQKPFQKGD QVRHNLFGLG IVLELERNNI IVEFVNPKKT KMFENSFKYL QKI
//
