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Database: UniProt/TrEMBL
Entry: V5ILI1_NEUCR
LinkDB: V5ILI1_NEUCR
Original site: V5ILI1_NEUCR 
ID   V5ILI1_NEUCR            Unreviewed;       462 AA.
AC   V5ILI1;
DT   22-JAN-2014, integrated into UniProtKB/TrEMBL.
DT   22-JAN-2014, sequence version 1.
DT   07-JUN-2017, entry version 34.
DE   RecName: Full=Isocitrate dehydrogenase [NADP] {ECO:0000256|PIRNR:PIRNR000108};
DE            EC=1.1.1.42 {ECO:0000256|PIRNR:PIRNR000108};
GN   Name=tca-5 {ECO:0000313|EMBL:ESA42220.1};
GN   ORFNames=NCU03857 {ECO:0000313|EMBL:ESA42220.1};
OS   Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM
OS   1257 / FGSC 987).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina;
OC   Sordariomycetes; Sordariomycetidae; Sordariales; Sordariaceae;
OC   Neurospora.
OX   NCBI_TaxID=367110 {ECO:0000313|EMBL:ESA42220.1, ECO:0000313|Proteomes:UP000001805};
RN   [1] {ECO:0000313|EMBL:ESA42220.1, ECO:0000313|Proteomes:UP000001805}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987
RC   {ECO:0000313|Proteomes:UP000001805}, and OR74A
RC   {ECO:0000313|EMBL:ESA42220.1};
RX   PubMed=12712197; DOI=10.1038/nature01554;
RA   Galagan J.E., Calvo S.E., Borkovich K.A., Selker E.U., Read N.D.,
RA   Jaffe D., FitzHugh W., Ma L.J., Smirnov S., Purcell S., Rehman B.,
RA   Elkins T., Engels R., Wang S., Nielsen C.B., Butler J., Endrizzi M.,
RA   Qui D., Ianakiev P., Bell-Pedersen D., Nelson M.A.,
RA   Werner-Washburne M., Selitrennikoff C.P., Kinsey J.A., Braun E.L.,
RA   Zelter A., Schulte U., Kothe G.O., Jedd G., Mewes W., Staben C.,
RA   Marcotte E., Greenberg D., Roy A., Foley K., Naylor J.,
RA   Stange-Thomann N., Barrett R., Gnerre S., Kamal M., Kamvysselis M.,
RA   Mauceli E., Bielke C., Rudd S., Frishman D., Krystofova S.,
RA   Rasmussen C., Metzenberg R.L., Perkins D.D., Kroken S., Cogoni C.,
RA   Macino G., Catcheside D., Li W., Pratt R.J., Osmani S.A.,
RA   DeSouza C.P., Glass L., Orbach M.J., Berglund J.A., Voelker R.,
RA   Yarden O., Plamann M., Seiler S., Dunlap J., Radford A., Aramayo R.,
RA   Natvig D.O., Alex L.A., Mannhaupt G., Ebbole D.J., Freitag M.,
RA   Paulsen I., Sachs M.S., Lander E.S., Nusbaum C., Birren B.;
RT   "The genome sequence of the filamentous fungus Neurospora crassa.";
RL   Nature 422:859-868(2003).
RN   [2] {ECO:0000313|EMBL:ESA42220.1}
RP   NUCLEOTIDE SEQUENCE.
RC   STRAIN=OR74A {ECO:0000313|EMBL:ESA42220.1};
RG   The Broad Institute Genome Sequencing Platform;
RA   Galagan J., Henn M.R., Hood H., Radford A., Collins R.A., Walker B.,
RA   Young S.K., Zeng Q., Gargeya S., Fitzgerald M., Haas B.,
RA   Abouelleil A., Allen A.W., Alvarado L., Arachchi H.M., Berlin A.,
RA   Chapman S.B., Chen Z., Gainer-Dewar J., Gnerre S., Goldberg J.,
RA   Griggs A., Gujja S., Hansen M., Howarth C., Imamovic A., Ireland A.,
RA   Larimer J.B., McCowan C., Murphy C., Pearson M.D., Poon T.W.,
RA   Priest M., Roberts A., Saif S., Shea T.D., Sisk P., Shea T., Sykes S.,
RA   Zucker J., Kodira C., Bowman B., Colot H., Ebbole D., Rasmussen C.,
RA   Baker C., Kalkman E., Chen C.-H., Shi M., Mathur R., Lambreghts R.,
RA   Collopy P., Mehra A., Schweredtfeger C., Hong C., Belden W.,
RA   Glass N.L., Borkovich K., Dunlap J., Lander E., Wortman J.,
RA   Nusbaum C., Sachs M., Birren B.;
RT   "The Genome Sequence of Neurospora crassa strain OR74A.";
RL   Submitted (APR-2013) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY: Isocitrate + NADP(+) = 2-oxoglutarate + CO(2)
CC       + NADPH. {ECO:0000256|PIRNR:PIRNR000108}.
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC         Evidence={ECO:0000256|PIRNR:PIRNR000108,
CC         ECO:0000256|PIRSR:PIRSR000108-3};
CC       Name=Mn(2+); Xref=ChEBI:CHEBI:29035;
CC         Evidence={ECO:0000256|PIRNR:PIRNR000108,
CC         ECO:0000256|PIRSR:PIRSR000108-3};
CC       Note=Binds 1 Mg(2+) or Mn(2+) ion per subunit.
CC       {ECO:0000256|PIRNR:PIRNR000108, ECO:0000256|PIRSR:PIRSR000108-3};
CC   -!- SIMILARITY: Belongs to the isocitrate and isopropylmalate
CC       dehydrogenases family. {ECO:0000256|PIRNR:PIRNR000108}.
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DR   EMBL; CM002241; ESA42218.1; -; Genomic_DNA.
DR   EMBL; CM002241; ESA42219.1; -; Genomic_DNA.
DR   EMBL; CM002241; ESA42220.1; -; Genomic_DNA.
DR   RefSeq; XP_011394871.1; XM_011396569.1.
DR   RefSeq; XP_011394872.1; XM_011396570.1.
DR   RefSeq; XP_011394873.1; XM_011396571.1.
DR   EnsemblFungi; ESA42218; ESA42218; NCU03857.
DR   EnsemblFungi; ESA42219; ESA42219; NCU03857.
DR   EnsemblFungi; ESA42220; ESA42220; NCU03857.
DR   GeneID; 3871997; -.
DR   KEGG; ncr:NCU03857; -.
DR   EuPathDB; FungiDB:NCU03857; -.
DR   KO; K00031; -.
DR   OMA; AMGMYNQ; -.
DR   OrthoDB; EOG092C2D51; -.
DR   Proteomes; UP000001805; Chromosome 5, Linkage Group VI.
DR   GO; GO:0004450; F:isocitrate dehydrogenase (NADP+) activity; IEA:UniProtKB-EC.
DR   GO; GO:0000287; F:magnesium ion binding; IEA:InterPro.
DR   GO; GO:0051287; F:NAD binding; IEA:InterPro.
DR   GO; GO:0006102; P:isocitrate metabolic process; IEA:InterPro.
DR   GO; GO:0006099; P:tricarboxylic acid cycle; IEA:UniProtKB-KW.
DR   InterPro; IPR019818; IsoCit/isopropylmalate_DH_CS.
DR   InterPro; IPR004790; Isocitrate_DH_NADP.
DR   InterPro; IPR024084; IsoPropMal-DH-like_dom.
DR   PANTHER; PTHR11822; PTHR11822; 1.
DR   Pfam; PF00180; Iso_dh; 1.
DR   PIRSF; PIRSF000108; IDH_NADP; 1.
DR   SMART; SM01329; Iso_dh; 1.
DR   TIGRFAMs; TIGR00127; nadp_idh_euk; 1.
DR   PROSITE; PS00470; IDH_IMDH; 1.
PE   3: Inferred from homology;
KW   Complete proteome {ECO:0000313|Proteomes:UP000001805};
KW   Magnesium {ECO:0000256|PIRNR:PIRNR000108,
KW   ECO:0000256|PIRSR:PIRSR000108-3};
KW   Manganese {ECO:0000256|PIRNR:PIRNR000108,
KW   ECO:0000256|PIRSR:PIRSR000108-3};
KW   Metal-binding {ECO:0000256|PIRNR:PIRNR000108,
KW   ECO:0000256|PIRSR:PIRSR000108-3};
KW   NADP {ECO:0000256|PIRNR:PIRNR000108, ECO:0000256|PIRSR:PIRSR000108-4};
KW   Oxidoreductase {ECO:0000256|PIRNR:PIRNR000108};
KW   Reference proteome {ECO:0000313|Proteomes:UP000001805};
KW   Tricarboxylic acid cycle {ECO:0000256|PIRNR:PIRNR000108}.
FT   DOMAIN       61    450       Iso_dh. {ECO:0000259|SMART:SM01329}.
FT   NP_BIND     127    129       NADP. {ECO:0000256|PIRSR:PIRSR000108-4}.
FT   NP_BIND     361    366       NADP. {ECO:0000256|PIRSR:PIRSR000108-4}.
FT   REGION      146    152       Substrate binding. {ECO:0000256|PIRSR:
FT                                PIRSR000108-2}.
FT   METAL       303    303       Magnesium or manganese.
FT                                {ECO:0000256|PIRSR:PIRSR000108-3}.
FT   METAL       326    326       Magnesium or manganese.
FT                                {ECO:0000256|PIRSR:PIRSR000108-3}.
FT   BINDING     129    129       Substrate. {ECO:0000256|PIRSR:
FT                                PIRSR000108-2}.
FT   BINDING     134    134       NADP. {ECO:0000256|PIRSR:PIRSR000108-4}.
FT   BINDING     161    161       Substrate. {ECO:0000256|PIRSR:
FT                                PIRSR000108-2}.
FT   BINDING     184    184       Substrate. {ECO:0000256|PIRSR:
FT                                PIRSR000108-2}.
FT   BINDING     311    311       NADP. {ECO:0000256|PIRSR:PIRSR000108-4}.
FT   BINDING     379    379       NADP; via amide nitrogen and carbonyl
FT                                oxygen. {ECO:0000256|PIRSR:PIRSR000108-
FT                                4}.
FT   SITE        191    191       Critical for catalysis.
FT                                {ECO:0000256|PIRSR:PIRSR000108-1}.
FT   SITE        263    263       Critical for catalysis.
FT                                {ECO:0000256|PIRSR:PIRSR000108-1}.
SQ   SEQUENCE   462 AA;  51848 MW;  64AC595FBD90F677 CRC64;
     MSPVTANLIR SSLRQASVYS RLNIATAPAF TRPAAPSLFV SNNRFRTMAS EAFQKIKVKN
     PVVELDGDEM TRIIWQDIKE KFIYPFLDID LKYYDLGLEY RDQTNDQVTI DAAEAIKKYS
     VGVKCATITP DESRVEEFKL KQMWLSPNGT IRNALGGTVF REPIVIPRVP RLVPGWKKPI
     IIGRHAFGDQ YRAKDLVAPG PGKLQMVYTP EGGEPQVIDV FDYKNGGGVA QTQYNTDESI
     EGFAHASFKL ALDKGLPLYM STKNTILKKY DGRFKDIFQQ IYDTQYKEAF EAKKIWYEHR
     LIDDMVAQMI KSSGGYIMAL KNYDGDVQSD IVAQGFGSLG LMTSVLITPD GKTFESEAAH
     GTVTRHYREH QKGNQTSTNP IASIFAWTRG LVQRGKLDNT PELIAFAEAL EKACIDTVDI
     DGIMTKDLAL ACGKTGREDW VTTSEYMAAV ERRLKSTLKE KL
//
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