ID V5RI98_SPIAP Unreviewed; 282 AA.
AC V5RI98;
DT 19-FEB-2014, integrated into UniProtKB/TrEMBL.
DT 19-FEB-2014, sequence version 1.
DT 24-JAN-2024, entry version 40.
DE RecName: Full=Type-2 restriction enzyme {ECO:0000256|PIRNR:PIRNR016080};
DE EC=3.1.21.4 {ECO:0000256|PIRNR:PIRNR016080};
GN ORFNames=SAPIS_v1c04340 {ECO:0000313|EMBL:AHB36279.1};
OS Spiroplasma apis B31.
OC Bacteria; Mycoplasmatota; Mollicutes; Entomoplasmatales; Spiroplasmataceae;
OC Spiroplasma.
OX NCBI_TaxID=1276258 {ECO:0000313|EMBL:AHB36279.1, ECO:0000313|Proteomes:UP000018550};
RN [1] {ECO:0000313|EMBL:AHB36279.1, ECO:0000313|Proteomes:UP000018550}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=B31 {ECO:0000313|EMBL:AHB36279.1};
RX PubMed=24407648;
RA Ku C., Lo W.S., Chen L.L., Kuo C.H.;
RT "Complete Genome Sequence of Spiroplasma apis B31T (ATCC 33834), a
RT Bacterium Associated with May Disease of Honeybees (Apis mellifera).";
RL Genome Announc. 2:e01151-13(2014).
CC -!- FUNCTION: A P subtype restriction enzyme that recognizes the double-
CC stranded unmethylated sequence 5'-GATC-3'.
CC {ECO:0000256|PIRNR:PIRNR016080}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Endonucleolytic cleavage of DNA to give specific double-
CC stranded fragments with terminal 5'-phosphates.; EC=3.1.21.4;
CC Evidence={ECO:0000256|PIRNR:PIRNR016080};
CC -!- SIMILARITY: Belongs to the DpnII type II restriction endonuclease
CC family. {ECO:0000256|PIRNR:PIRNR016080}.
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DR EMBL; CP006682; AHB36279.1; -; Genomic_DNA.
DR RefSeq; WP_023789217.1; NC_022998.1.
DR AlphaFoldDB; V5RI98; -.
DR STRING; 1276258.SAPIS_v1c04340; -.
DR REBASE; 74496; SapB31ORF4350P.
DR KEGG; sapi:SAPIS_v1c04340; -.
DR PATRIC; fig|1276258.3.peg.435; -.
DR eggNOG; ENOG502Z7V5; Bacteria.
DR HOGENOM; CLU_089327_0_0_14; -.
DR OrthoDB; 9771872at2; -.
DR Proteomes; UP000018550; Chromosome.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0009036; F:type II site-specific deoxyribonuclease activity; IEA:UniProtKB-UniRule.
DR GO; GO:0009307; P:DNA restriction-modification system; IEA:UniProtKB-UniRule.
DR InterPro; IPR011335; Restrct_endonuc-II-like.
DR InterPro; IPR021191; Restrct_endonuc_II_DpnII.
DR InterPro; IPR007637; Restrct_endonuc_II_DpnII-like.
DR Pfam; PF04556; DpnII; 1.
DR PIRSF; PIRSF016080; Restrict_endonuc_II_DpmII; 1.
DR SUPFAM; SSF52980; Restriction endonuclease-like; 1.
PE 3: Inferred from homology;
KW Endonuclease {ECO:0000256|PIRNR:PIRNR016080};
KW Hydrolase {ECO:0000256|PIRNR:PIRNR016080};
KW Nuclease {ECO:0000256|PIRNR:PIRNR016080};
KW Reference proteome {ECO:0000313|Proteomes:UP000018550};
KW Restriction system {ECO:0000256|PIRNR:PIRNR016080}.
FT DOMAIN 4..277
FT /note="Restriction endonuclease type II DpnII-like"
FT /evidence="ECO:0000259|Pfam:PF04556"
SQ SEQUENCE 282 AA; 32984 MW; FD77ABC0E828EDAA CRC64;
MKKNFDNFIN NFKDKVFNLD FYVDYDKAIK NVDIYKKEIE VLNKAILAKE PLHILKLAFE
ENSNLQKLIP SLIAVRKNVI QTISESYDFS KKMDANKIIE FIDGVGLLKI IQNNRITNFN
DYLLGLEVGM DTNARKNRTG KAMESLVSDL LIEKGLIFSE QVSLRNIANI IPDFDWEDLS
SQFDKFNADK RVDFLFKYNE IYYLTEVNFY SSGGSKLNET TRSFIMLNNK LKKYEHIKFL
WITDGIGWLT AKKQIREAYE KIDLLFNLKE FEENSLEDLL GI
//
