ID V9WMZ2_9RHOB Unreviewed; 639 AA.
AC V9WMZ2;
DT 19-MAR-2014, integrated into UniProtKB/TrEMBL.
DT 19-MAR-2014, sequence version 1.
DT 24-JAN-2024, entry version 37.
DE RecName: Full=Beta-galactosidase {ECO:0000256|ARBA:ARBA00012756, ECO:0000256|PIRNR:PIRNR001084};
DE Short=Beta-gal {ECO:0000256|PIRNR:PIRNR001084};
DE EC=3.2.1.23 {ECO:0000256|ARBA:ARBA00012756, ECO:0000256|PIRNR:PIRNR001084};
GN ORFNames=Gal_02759 {ECO:0000313|EMBL:AHD10492.1};
OS Phaeobacter gallaeciensis DSM 26640.
OC Bacteria; Pseudomonadota; Alphaproteobacteria; Rhodobacterales;
OC Roseobacteraceae; Phaeobacter.
OX NCBI_TaxID=1423144 {ECO:0000313|EMBL:AHD10492.1, ECO:0000313|Proteomes:UP000018782};
RN [1] {ECO:0000313|EMBL:AHD10492.1, ECO:0000313|Proteomes:UP000018782}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 26640 {ECO:0000313|EMBL:AHD10492.1,
RC ECO:0000313|Proteomes:UP000018782};
RA Frank O., Petersen J., Goeker M., Klenk H.-P., Rohde M., Scheuner C.;
RT "Complete genome sequence of the Phaeobacter gallaeciensis type strain CIP
RT 105210T.";
RL Submitted (DEC-2013) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Hydrolysis of terminal non-reducing beta-D-galactose residues
CC in beta-D-galactosides.; EC=3.2.1.23;
CC Evidence={ECO:0000256|ARBA:ARBA00001412,
CC ECO:0000256|PIRNR:PIRNR001084};
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 42 family.
CC {ECO:0000256|ARBA:ARBA00005940, ECO:0000256|PIRNR:PIRNR001084}.
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DR EMBL; CP006966; AHD10492.1; -; Genomic_DNA.
DR RefSeq; WP_024098123.1; NZ_AOQA01000007.1.
DR AlphaFoldDB; V9WMZ2; -.
DR GeneID; 31847127; -.
DR KEGG; pgd:Gal_02759; -.
DR PATRIC; fig|1423144.3.peg.2763; -.
DR eggNOG; COG1874; Bacteria.
DR HOGENOM; CLU_012430_1_1_5; -.
DR OrthoDB; 9800974at2; -.
DR Proteomes; UP000018782; Chromosome.
DR GO; GO:0009341; C:beta-galactosidase complex; IEA:InterPro.
DR GO; GO:0004565; F:beta-galactosidase activity; IEA:UniProtKB-EC.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR CDD; cd03143; A4_beta-galactosidase_middle_domain; 1.
DR Gene3D; 3.40.50.880; -; 1.
DR Gene3D; 3.20.20.80; Glycosidases; 1.
DR InterPro; IPR013738; Beta_galactosidase_Trimer.
DR InterPro; IPR029062; Class_I_gatase-like.
DR InterPro; IPR003476; Glyco_hydro_42.
DR InterPro; IPR013529; Glyco_hydro_42_N.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR PANTHER; PTHR36447; BETA-GALACTOSIDASE GANA; 1.
DR PANTHER; PTHR36447:SF2; BETA-GALACTOSIDASE YESZ; 1.
DR Pfam; PF02449; Glyco_hydro_42; 1.
DR Pfam; PF08532; Glyco_hydro_42M; 1.
DR PIRSF; PIRSF001084; B-galactosidase; 1.
DR SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR SUPFAM; SSF52317; Class I glutamine amidotransferase-like; 1.
PE 3: Inferred from homology;
KW Glycosidase {ECO:0000256|PIRNR:PIRNR001084, ECO:0000313|EMBL:AHD10492.1};
KW Hydrolase {ECO:0000256|PIRNR:PIRNR001084, ECO:0000313|EMBL:AHD10492.1};
KW Metal-binding {ECO:0000256|PIRSR:PIRSR001084-3};
KW Reference proteome {ECO:0000313|Proteomes:UP000018782};
KW Zinc {ECO:0000256|PIRSR:PIRSR001084-3}.
FT DOMAIN 8..393
FT /note="Glycoside hydrolase family 42 N-terminal"
FT /evidence="ECO:0000259|Pfam:PF02449"
FT DOMAIN 402..591
FT /note="Beta-galactosidase trimerisation"
FT /evidence="ECO:0000259|Pfam:PF08532"
FT ACT_SITE 144
FT /note="Proton donor"
FT /evidence="ECO:0000256|PIRSR:PIRSR001084-1"
FT ACT_SITE 316
FT /note="Nucleophile"
FT /evidence="ECO:0000256|PIRSR:PIRSR001084-1"
FT BINDING 105
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR001084-2"
FT BINDING 109
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000256|PIRSR:PIRSR001084-3"
FT BINDING 143
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR001084-2"
FT BINDING 324
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR001084-2"
SQ SEQUENCE 639 AA; 71503 MW; 5A83C2A722C20CA7 CRC64;
MQRTLGTCYY PEHWPEDIWQ EDAARMVAAG LTWVRIGEFS WSRIEPTPGD LQWDWLDRAI
NTLGAAGLKV VLGTPTATPP RWMVDRHPDM FALDADGKPR GFGSRRHYCF SHKGYRAEAR
RIVTLMAERY GPGGRIAAWQ TDNEYGCHDT TISYSDSARV AFVRWLEERY NGDICALNAA
WGNVFWSMEY ERFDQIGLPN LTVTEPNPAH VQAFRRFSSD QVVSFNRDQV EILRAHSDAP
IAHNYMGRVT DFDHYKVGVD LEIASWDSYP LGFLEDRVGA SPEDQRRYAR QGDPDFQAFH
HDLYRAVGRG RWWVMEQQPG PVNWAPYNPA PLPGMVRFWT WEAFAHGAEA VCYFRWRQAP
FAQEQLHAGL LRPDSAETPA ISEARQVAEE LASAGEVGPS QAPVALIFDY DAEWAWQVQP
HGQGLSYFDL VLDHYRALRR AGLSVDIVPP TQSDFTGYEL ILAPGLMHLP DDLRRALADA
PGLRLYGPRT AARDEDFKIP TAPLPPALQG LDVVIAAVES LRPDMPLPLA NGDGAVVCYL
ETLEGEADVL FETSTGHAVA VAAGNQVYCA GWLDMAGLDC LIATLCARAG VTIRKMPQGV
RVRQTATEEF WFNHLAEPVE AVVGTLPPAG VLRRARQEV
//
