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Database: UniProt/TrEMBL
Entry: W8ZJ87_ECOLX
LinkDB: W8ZJ87_ECOLX
Original site: W8ZJ87_ECOLX 
ID   W8ZJ87_ECOLX            Unreviewed;       495 AA.
AC   W8ZJ87;
DT   14-MAY-2014, integrated into UniProtKB/TrEMBL.
DT   14-MAY-2014, sequence version 1.
DT   27-SEP-2017, entry version 25.
DE   SubName: Full=Cytoplasmic alpha-amylase {ECO:0000313|EMBL:CDN82488.1};
DE            EC=3.2.1.1 {ECO:0000313|EMBL:OLR34230.1};
GN   Name=amyA {ECO:0000313|EMBL:CDN82488.1};
GN   ORFNames=EC958_2195 {ECO:0000313|EMBL:CDN82488.1}, UG58_20140
GN   {ECO:0000313|EMBL:OLR34230.1};
OS   Escherichia coli O25b:H4-ST131.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=941322 {ECO:0000313|EMBL:CDN82488.1, ECO:0000313|Proteomes:UP000032727};
RN   [1] {ECO:0000313|EMBL:CDN82488.1}
RP   NUCLEOTIDE SEQUENCE.
RC   STRAIN=EC958 {ECO:0000313|EMBL:CDN82488.1};
RA   Beatson S.;
RL   Submitted (OCT-2011) to the EMBL/GenBank/DDBJ databases.
RN   [2] {ECO:0000313|EMBL:CDN82488.1, ECO:0000313|Proteomes:UP000032727}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=EC958 {ECO:0000313|EMBL:CDN82488.1,
RC   ECO:0000313|Proteomes:UP000032727};
RX   PubMed=25126841; DOI=10.1371/journal.pone.0104400;
RA   Forde B.M., Ben Zakour N.L., Stanton-Cook M., Phan M.D., Totsika M.,
RA   Peters K.M., Chan K.G., Schembri M.A., Upton M., Beatson S.A.;
RT   "The complete genome sequence of Escherichia coli EC958: a high
RT   quality reference sequence for the globally disseminated multidrug
RT   resistant E. coli O25b:H4-ST131 clone.";
RL   PLoS ONE 9:e104400-e104400(2014).
RN   [3] {ECO:0000313|EMBL:OLR34230.1, ECO:0000313|Proteomes:UP000185771}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=UR40 {ECO:0000313|EMBL:OLR34230.1,
RC   ECO:0000313|Proteomes:UP000185771};
RA   Brochmann R.P., Jana B., Gumpert H.K., Guardabassi L.;
RT   "Escherichia coli UR40, ST131 harboring plasmids belonging to the IncF
RT   group encoding the bla(CTX-M-15) gene.";
RL   Submitted (APR-2015) to the EMBL/GenBank/DDBJ databases.
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DR   EMBL; HG941718; CDN82488.1; -; Genomic_DNA.
DR   EMBL; LAWT01000019; OLR34230.1; -; Genomic_DNA.
DR   RefSeq; WP_001245699.1; NZ_LAWT01000019.1.
DR   ProteinModelPortal; W8ZJ87; -.
DR   EnsemblBacteria; CDN82488; CDN82488; EC958_2195.
DR   KEGG; ecos:EC958_2195; -.
DR   KO; K01176; -.
DR   Proteomes; UP000032727; Chromosome.
DR   Proteomes; UP000185771; Unassembled WGS sequence.
DR   GO; GO:0004556; F:alpha-amylase activity; IEA:UniProtKB-EC.
DR   GO; GO:0103025; F:alpha-amylase activity (releasing maltohexaose); IEA:UniProtKB-EC.
DR   GO; GO:0005509; F:calcium ion binding; IEA:InterPro.
DR   GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR   InterPro; IPR006048; A-amylase/branching_C.
DR   InterPro; IPR013776; A-amylase_thermo.
DR   InterPro; IPR006047; Glyco_hydro_13_cat_dom.
DR   InterPro; IPR017853; Glycoside_hydrolase_SF.
DR   Pfam; PF00128; Alpha-amylase; 1.
DR   Pfam; PF02806; Alpha-amylase_C; 1.
DR   PIRSF; PIRSF001021; Alph-amls_thrmst; 1.
DR   SMART; SM00642; Aamy; 1.
DR   SUPFAM; SSF51445; SSF51445; 1.
PE   4: Predicted;
KW   Calcium {ECO:0000256|PIRSR:PIRSR001021-2};
KW   Complete proteome {ECO:0000313|Proteomes:UP000032727,
KW   ECO:0000313|Proteomes:UP000185771};
KW   Glycosidase {ECO:0000313|EMBL:OLR34230.1};
KW   Hydrolase {ECO:0000313|EMBL:OLR34230.1};
KW   Metal-binding {ECO:0000256|PIRSR:PIRSR001021-2}.
FT   DOMAIN        4    402       Aamy. {ECO:0000259|SMART:SM00642}.
FT   ACT_SITE    235    235       Nucleophile. {ECO:0000256|PIRSR:
FT                                PIRSR001021-1}.
FT   ACT_SITE    265    265       Proton donor. {ECO:0000256|PIRSR:
FT                                PIRSR001021-1}.
FT   METAL       104    104       Calcium 1. {ECO:0000256|PIRSR:
FT                                PIRSR001021-2}.
FT   METAL       198    198       Calcium 1. {ECO:0000256|PIRSR:
FT                                PIRSR001021-2}.
FT   METAL       206    206       Calcium 2. {ECO:0000256|PIRSR:
FT                                PIRSR001021-2}.
FT   METAL       239    239       Calcium 1; via carbonyl oxygen.
FT                                {ECO:0000256|PIRSR:PIRSR001021-2}.
SQ   SEQUENCE   495 AA;  56586 MW;  26BD74E87F49C4D6 CRC64;
     MRNPTLLQCF HWYYPEGGKL WPELAERADG FNDIGINMVW LPPAYKGASG GYSVGYDSYD
     LFDLGEFDQK GSIPTKYGDK AQLLAAIDAL KRNDIAVLLD VVVNHKMGAD EKEAIRVQRV
     NADDRTQIDE EIIECEGWTR YTFPARAGQY SQFIWDFKCF SGIDHIENPD EDGIFKIVND
     YTGEGWNDQV DDELGNFDYL MGENIDFRNH AVTEEIKYWA RWVMEQTQCD GFRLDAVKHI
     PAWFYKEWIE HVQEVAPKPL FIVAEYWSHE VDKLQTYIDQ VEGKTMLFDA PLQMKFHEAS
     RMGRDYDMTQ IFTGTLVEAD PFHAVTLVAN HDTQPLQALE APVEPWFKPL AYALILLREN
     GVPSVFYPDL YGAHYEDVGG DGQTYPIDMP IIEQLDELIL ARQRFAHGVQ TLFFDHPNCI
     AFSRSGTDEY PGCVVVMSNG DDGEKTIHLG ENYGNKTWRD FLGNRQESVV TDENGEATFF
     CNGGSVSVWV IEEVI
//
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