ID A0A022PU07_ERYGU Unreviewed; 742 AA.
AC A0A022PU07;
DT 11-JUN-2014, integrated into UniProtKB/TrEMBL.
DT 11-JUN-2014, sequence version 1.
DT 27-MAR-2024, entry version 34.
DE RecName: Full=Long-chain-alcohol oxidase {ECO:0000256|ARBA:ARBA00013125, ECO:0000256|PIRNR:PIRNR028937};
DE EC=1.1.3.20 {ECO:0000256|ARBA:ARBA00013125, ECO:0000256|PIRNR:PIRNR028937};
GN ORFNames=MIMGU_mgv1a001896mg {ECO:0000313|EMBL:EYU18303.1};
OS Erythranthe guttata (Yellow monkey flower) (Mimulus guttatus).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC asterids; lamiids; Lamiales; Phrymaceae; Erythranthe.
OX NCBI_TaxID=4155 {ECO:0000313|EMBL:EYU18303.1, ECO:0000313|Proteomes:UP000030748};
RN [1] {ECO:0000313|EMBL:EYU18303.1, ECO:0000313|Proteomes:UP000030748}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. DUN x IM62 {ECO:0000313|Proteomes:UP000030748};
RX PubMed=24225854; DOI=10.1073/pnas.1319032110;
RA Hellsten U., Wright K.M., Jenkins J., Shu S., Yuan Y., Wessler S.R.,
RA Schmutz J., Willis J.H., Rokhsar D.S.;
RT "Fine-scale variation in meiotic recombination in Mimulus inferred from
RT population shotgun sequencing.";
RL Proc. Natl. Acad. Sci. U.S.A. 110:19478-19482(2013).
CC -!- FUNCTION: Long-chain fatty alcohol oxidase involved in the omega-
CC oxidation pathway of lipid degradation. {ECO:0000256|ARBA:ARBA00003842,
CC ECO:0000256|PIRNR:PIRNR028937}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a long-chain primary fatty alcohol + O2 = a long-chain fatty
CC aldehyde + H2O2; Xref=Rhea:RHEA:22756, ChEBI:CHEBI:15379,
CC ChEBI:CHEBI:16240, ChEBI:CHEBI:17176, ChEBI:CHEBI:77396; EC=1.1.3.20;
CC Evidence={ECO:0000256|ARBA:ARBA00000920,
CC ECO:0000256|PIRNR:PIRNR028937};
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000256|PIRNR:PIRNR028937}.
CC -!- SIMILARITY: Belongs to the GMC oxidoreductase family.
CC {ECO:0000256|ARBA:ARBA00010790, ECO:0000256|PIRNR:PIRNR028937}.
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DR EMBL; KI632336; EYU18303.1; -; Genomic_DNA.
DR RefSeq; XP_012828584.1; XM_012973130.1.
DR AlphaFoldDB; A0A022PU07; -.
DR STRING; 4155.A0A022PU07; -.
DR GeneID; 105949820; -.
DR KEGG; egt:105949820; -.
DR eggNOG; ENOG502QSD8; Eukaryota.
DR OMA; YGVIYET; -.
DR OrthoDB; 601859at2759; -.
DR PhylomeDB; A0A022PU07; -.
DR Proteomes; UP000030748; Unassembled WGS sequence.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0050660; F:flavin adenine dinucleotide binding; IEA:InterPro.
DR GO; GO:0046577; F:long-chain-alcohol oxidase activity; IEA:UniProtKB-EC.
DR Gene3D; 3.50.50.60; FAD/NAD(P)-binding domain; 2.
DR InterPro; IPR036188; FAD/NAD-bd_sf.
DR InterPro; IPR000172; GMC_OxRdtase_N.
DR InterPro; IPR007867; GMC_OxRtase_C.
DR InterPro; IPR012400; Long_Oxdase.
DR PANTHER; PTHR46056; LONG-CHAIN-ALCOHOL OXIDASE; 1.
DR PANTHER; PTHR46056:SF10; LONG-CHAIN-ALCOHOL OXIDASE FAO3; 1.
DR Pfam; PF05199; GMC_oxred_C; 1.
DR Pfam; PF00732; GMC_oxred_N; 1.
DR Pfam; PF13450; NAD_binding_8; 1.
DR PIRSF; PIRSF028937; Lg_Ch_AO; 1.
DR SUPFAM; SSF51905; FAD/NAD(P)-binding domain; 1.
PE 3: Inferred from homology;
KW FAD {ECO:0000256|PIRSR:PIRSR028937-2};
KW Flavoprotein {ECO:0000256|PIRSR:PIRSR028937-2};
KW Membrane {ECO:0000256|ARBA:ARBA00023136, ECO:0000256|PIRNR:PIRNR028937};
KW Oxidoreductase {ECO:0000256|PIRNR:PIRNR028937};
KW Reference proteome {ECO:0000313|Proteomes:UP000030748};
KW Transmembrane {ECO:0000256|ARBA:ARBA00022692};
KW Transmembrane helix {ECO:0000256|ARBA:ARBA00022989}.
FT DOMAIN 288..508
FT /note="Glucose-methanol-choline oxidoreductase N-terminal"
FT /evidence="ECO:0000259|Pfam:PF00732"
FT DOMAIN 599..732
FT /note="Glucose-methanol-choline oxidoreductase C-terminal"
FT /evidence="ECO:0000259|Pfam:PF05199"
FT ACT_SITE 678
FT /note="Proton acceptor"
FT /evidence="ECO:0000256|PIRSR:PIRSR028937-1"
FT BINDING 240..255
FT /ligand="FAD"
FT /ligand_id="ChEBI:CHEBI:57692"
FT /evidence="ECO:0000256|PIRSR:PIRSR028937-2"
SQ SEQUENCE 742 AA; 80468 MW; 7DC1C9EBBF21492D CRC64;
MGRGDCHHPL LKGGRRETKY SHGFSSSQIQ TLTSICEVLL PPTPLNNPHE NINPQNDVVQ
AFYKASASQY PVPDEVAELV MKRAFFEAKI LVIAIVRILS TRVGTLLICG SSGVSKKWPF
LKKFSEIPLE NREKVVQKWF TNWLLTPVRL AFVFLKFLVL LVFFTQVGED SSNPAWKAMD
YKVDVIGENS PEEMSKERPL DKGIIETRLE TDSSLAAALA GKGLKPTLNP ASRDTYTVKC
DAVIVGSGCG GGVAAAVLAA AGLKVVVLEK GDYFTAGDYS SLEGPSLDRL YESGGILSTL
DGKTMLLAGS TVGGGSAVNW SACFKTPDHI INDWAHGQNL PLFSGPEYLD AMDKVCDRIG
VTVGCTKEGF QNQILRQGCE NLGLKADPVP RNSSESHYCG SCCYGCIRGD KKGTDTTWLV
DAVNSDAVII SGAKAVRFVI NQNNKNQRKK KKCHGVIAKF TNQDIKITLR IEARVTISAC
GALLTPPLLI NSGLKNRNIG RNLHLHPVLM AWGYFPEPDI VEGKAYEGGI ITSVHKVVEQ
DKVRAIIESP ILGPGSFAAL LPFESGIELK NRIIKYSRTC HLFSMIKDNG SGSVLSEGRI
DYKLDSSDKE NMKIGLVRAL RILIAAGAVE VGTHQSDGQR VKCEGGDDEG VEEFLKGVVA
PEGPKSMVEK WSTYCSAHQM GSCRMGVDER CGAVDTWGES WEAEGLFVCD ASVLPGAVGV
NPMITIQATA YCISKKIAEK LK
//
