ID A0A045HBR7_MYCTX Unreviewed; 188 AA.
AC A0A045HBR7;
DT 09-JUL-2014, integrated into UniProtKB/TrEMBL.
DT 09-JUL-2014, sequence version 1.
DT 27-MAR-2024, entry version 79.
DE RecName: Full=Crossover junction endodeoxyribonuclease RuvC {ECO:0000256|HAMAP-Rule:MF_00034};
DE EC=3.1.21.10 {ECO:0000256|HAMAP-Rule:MF_00034};
DE AltName: Full=Holliday junction nuclease RuvC {ECO:0000256|HAMAP-Rule:MF_00034};
DE AltName: Full=Holliday junction resolvase RuvC {ECO:0000256|HAMAP-Rule:MF_00034};
GN Name=ruvC {ECO:0000256|HAMAP-Rule:MF_00034,
GN ECO:0000313|EMBL:CNU72055.1};
GN ORFNames=A4S10_02719 {ECO:0000313|EMBL:OMH60539.1}, ERS007657_01518
GN {ECO:0000313|EMBL:CFR76872.1}, ERS007661_01431
GN {ECO:0000313|EMBL:CNU96322.1}, ERS007665_01523
GN {ECO:0000313|EMBL:CNU72055.1}, ERS007679_01901
GN {ECO:0000313|EMBL:COV46596.1}, ERS007681_01884
GN {ECO:0000313|EMBL:CFE39504.1}, ERS007688_01325
GN {ECO:0000313|EMBL:CFE49039.1}, ERS007741_03092
GN {ECO:0000313|EMBL:COW75824.1}, ERS027646_01135
GN {ECO:0000313|EMBL:CKS02927.1}, ERS027659_01061
GN {ECO:0000313|EMBL:CKR27972.1}, ERS027661_02512
GN {ECO:0000313|EMBL:CKS04672.1}, ERS053720_02570
GN {ECO:0000313|EMBL:CFH85719.1}, GJE03_13660
GN {ECO:0000313|EMBL:QGK79766.1}, SAMEA2683035_01865
GN {ECO:0000313|EMBL:SGH73136.1};
OS Mycobacterium tuberculosis.
OC Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales; Mycobacteriaceae;
OC Mycobacterium; Mycobacterium tuberculosis complex.
OX NCBI_TaxID=1773 {ECO:0000313|EMBL:CNU72055.1, ECO:0000313|Proteomes:UP000045274};
RN [1] {ECO:0000313|Proteomes:UP000039217, ECO:0000313|Proteomes:UP000045274}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=2926STDY5723788 {ECO:0000313|EMBL:SGH73136.1,
RC ECO:0000313|Proteomes:UP000182946}, Bir 172
RC {ECO:0000313|EMBL:CKS02927.1, ECO:0000313|Proteomes:UP000048948}, Bir
RC 185 {ECO:0000313|EMBL:CKR27972.1, ECO:0000313|Proteomes:UP000050164},
RC Bir 187 {ECO:0000313|EMBL:CKS04672.1,
RC ECO:0000313|Proteomes:UP000049023}, C09601061
RC {ECO:0000313|EMBL:CFR76872.1, ECO:0000313|Proteomes:UP000046680},
RC D00501624 {ECO:0000313|EMBL:CNU96322.1,
RC ECO:0000313|Proteomes:UP000039217}, D00700688
RC {ECO:0000313|EMBL:CNU72055.1, ECO:0000313|Proteomes:UP000045274},
RC G09801536 {ECO:0000313|EMBL:COV46596.1,
RC ECO:0000313|Proteomes:UP000045842}, G09901357
RC {ECO:0000313|EMBL:CFE39504.1, ECO:0000313|Proteomes:UP000048289},
RC H09601792 {ECO:0000313|EMBL:CFE49039.1,
RC ECO:0000313|Proteomes:UP000046947}, and P00601463
RC {ECO:0000313|EMBL:COW75824.1, ECO:0000313|Proteomes:UP000048600};
RG Pathogen Informatics;
RL Submitted (MAR-2015) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|EMBL:CFH85719.1, ECO:0000313|Proteomes:UP000046674}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=0351H {ECO:0000313|EMBL:CFH85719.1,
RC ECO:0000313|Proteomes:UP000046674};
RG Pathogen Informatics;
RA Murphy D.;
RL Submitted (MAR-2015) to the EMBL/GenBank/DDBJ databases.
RN [3] {ECO:0000313|EMBL:OMH60539.1, ECO:0000313|Proteomes:UP000189452}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=6548 {ECO:0000313|EMBL:OMH60539.1,
RC ECO:0000313|Proteomes:UP000189452};
RA Bigi M., Bigi F., Soria M.A.;
RL Submitted (APR-2016) to the EMBL/GenBank/DDBJ databases.
RN [4] {ECO:0000313|EMBL:OMH60539.1, ECO:0000313|Proteomes:UP000189452}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=6548 {ECO:0000313|EMBL:OMH60539.1,
RC ECO:0000313|Proteomes:UP000189452};
RA Bigi M.M., Lopez B., Blanco F.C., Sasiain M.C., De La Barrera S.,
RA Ritacco V., Bigi F., Soria M.A.;
RT "Protein polymorphisms may explain contrasting epidemiological fitness of
RT two variants of a multidrug-resistant Mycobacterium tuberculosis strain.";
RL Submitted (FEB-2017) to the EMBL/GenBank/DDBJ databases.
RN [5] {ECO:0000313|EMBL:QGK79766.1, ECO:0000313|Proteomes:UP000388593}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AUSMDU00018547 {ECO:0000313|EMBL:QGK79766.1,
RC ECO:0000313|Proteomes:UP000388593};
RA Baines S.L., Goncalves da Silva A., Carter G.P., Jennison A.V.,
RA Rathnayake I.U., Graham R.M.A., Sintchenko V., Wang Q., Rockett R.J.,
RA Timms V.J., Martinez E., Ballard S., Tomita T., Isles N., Horan K.A.,
RA Pitchers W., Stinear T.P., Williamson D.A., Howden B.P., Seemann T.;
RT "Complete microbial genomes for public health in Australia and Southwest
RT Pacific.";
RL Submitted (NOV-2019) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: The RuvA-RuvB-RuvC complex processes Holliday junction (HJ)
CC DNA during genetic recombination and DNA repair. Endonuclease that
CC resolves HJ intermediates. Cleaves cruciform DNA by making single-
CC stranded nicks across the HJ at symmetrical positions within the
CC homologous arms, yielding a 5'-phosphate and a 3'-hydroxyl group;
CC requires a central core of homology in the junction. The consensus
CC cleavage sequence is 5'-(A/T)TT(C/G)-3'. Cleavage occurs on the 3'-side
CC of the TT dinucleotide at the point of strand exchange. HJ branch
CC migration catalyzed by RuvA-RuvB allows RuvC to scan DNA until it finds
CC its consensus sequence, where it cleaves and resolves the cruciform
CC DNA. {ECO:0000256|HAMAP-Rule:MF_00034}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Endonucleolytic cleavage at a junction such as a reciprocal
CC single-stranded crossover between two homologous DNA duplexes
CC (Holliday junction).; EC=3.1.21.10; Evidence={ECO:0000256|HAMAP-
CC Rule:MF_00034};
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000256|HAMAP-Rule:MF_00034};
CC Note=Binds 2 Mg(2+) ion per subunit. {ECO:0000256|HAMAP-Rule:MF_00034};
CC -!- SUBUNIT: Homodimer which binds Holliday junction (HJ) DNA. The HJ
CC becomes 2-fold symmetrical on binding to RuvC with unstacked arms; it
CC has a different conformation from HJ DNA in complex with RuvA. In the
CC full resolvosome a probable DNA-RuvA(4)-RuvB(12)-RuvC(2) complex forms
CC which resolves the HJ. {ECO:0000256|HAMAP-Rule:MF_00034}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00034}.
CC -!- SIMILARITY: Belongs to the RuvC family. {ECO:0000256|ARBA:ARBA00009518,
CC ECO:0000256|HAMAP-Rule:MF_00034}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; CFOE01000213; CFE39504.1; -; Genomic_DNA.
DR EMBL; CFOH01000163; CFE49039.1; -; Genomic_DNA.
DR EMBL; CFSW01000015; CFH85719.1; -; Genomic_DNA.
DR EMBL; CGCX01000474; CFR76872.1; -; Genomic_DNA.
DR EMBL; CNFT01000175; CKR27972.1; -; Genomic_DNA.
DR EMBL; CNGE01000150; CKS02927.1; -; Genomic_DNA.
DR EMBL; CNFU01000538; CKS04672.1; -; Genomic_DNA.
DR EMBL; CQPW01000255; CNU72055.1; -; Genomic_DNA.
DR EMBL; CQQC01000394; CNU96322.1; -; Genomic_DNA.
DR EMBL; CSAD01000229; COV46596.1; -; Genomic_DNA.
DR EMBL; CHKL01000426; COW75824.1; -; Genomic_DNA.
DR EMBL; LWDQ01000001; OMH60539.1; -; Genomic_DNA.
DR EMBL; CP045962; QGK79766.1; -; Genomic_DNA.
DR EMBL; FPTZ01000011; SGH73136.1; -; Genomic_DNA.
DR RefSeq; WP_003413426.1; NZ_WUCS01000012.1.
DR GeneID; 45426596; -.
DR PATRIC; fig|1773.206.peg.3013; -.
DR OMA; AICHIWR; -.
DR Proteomes; UP000039217; Unassembled WGS sequence.
DR Proteomes; UP000045274; Unassembled WGS sequence.
DR Proteomes; UP000045842; Unassembled WGS sequence.
DR Proteomes; UP000046674; Unassembled WGS sequence.
DR Proteomes; UP000046680; Unassembled WGS sequence.
DR Proteomes; UP000046947; Unassembled WGS sequence.
DR Proteomes; UP000048289; Unassembled WGS sequence.
DR Proteomes; UP000048600; Unassembled WGS sequence.
DR Proteomes; UP000048948; Unassembled WGS sequence.
DR Proteomes; UP000049023; Unassembled WGS sequence.
DR Proteomes; UP000050164; Unassembled WGS sequence.
DR Proteomes; UP000182946; Unassembled WGS sequence.
DR Proteomes; UP000189452; Chromosome.
DR Proteomes; UP000388593; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0048476; C:Holliday junction resolvase complex; IEA:UniProtKB-UniRule.
DR GO; GO:0008821; F:crossover junction DNA endonuclease activity; IEA:UniProtKB-UniRule.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0000287; F:magnesium ion binding; IEA:UniProtKB-UniRule.
DR GO; GO:0006310; P:DNA recombination; IEA:UniProtKB-UniRule.
DR GO; GO:0006281; P:DNA repair; IEA:UniProtKB-UniRule.
DR CDD; cd16962; RuvC; 1.
DR Gene3D; 3.30.420.10; Ribonuclease H-like superfamily/Ribonuclease H; 1.
DR HAMAP; MF_00034; RuvC; 1.
DR InterPro; IPR012337; RNaseH-like_sf.
DR InterPro; IPR036397; RNaseH_sf.
DR InterPro; IPR020563; X-over_junc_endoDNase_Mg_BS.
DR InterPro; IPR002176; X-over_junc_endoDNase_RuvC.
DR NCBIfam; TIGR00228; ruvC; 1.
DR PANTHER; PTHR30194; CROSSOVER JUNCTION ENDODEOXYRIBONUCLEASE RUVC; 1.
DR PANTHER; PTHR30194:SF3; CROSSOVER JUNCTION ENDODEOXYRIBONUCLEASE RUVC; 1.
DR Pfam; PF02075; RuvC; 1.
DR PRINTS; PR00696; RSOLVASERUVC.
DR SUPFAM; SSF53098; Ribonuclease H-like; 1.
DR PROSITE; PS01321; RUVC; 1.
PE 3: Inferred from homology;
KW Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00034};
KW DNA damage {ECO:0000256|ARBA:ARBA00022763, ECO:0000256|HAMAP-
KW Rule:MF_00034};
KW DNA recombination {ECO:0000256|ARBA:ARBA00023172, ECO:0000256|HAMAP-
KW Rule:MF_00034};
KW DNA repair {ECO:0000256|ARBA:ARBA00023204, ECO:0000256|HAMAP-
KW Rule:MF_00034};
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125, ECO:0000256|HAMAP-
KW Rule:MF_00034};
KW Endonuclease {ECO:0000256|ARBA:ARBA00022759, ECO:0000256|HAMAP-
KW Rule:MF_00034};
KW Hydrolase {ECO:0000256|HAMAP-Rule:MF_00034, ECO:0000313|EMBL:CNU72055.1};
KW Magnesium {ECO:0000256|ARBA:ARBA00022842, ECO:0000256|HAMAP-Rule:MF_00034};
KW Metal-binding {ECO:0000256|HAMAP-Rule:MF_00034};
KW Nuclease {ECO:0000256|HAMAP-Rule:MF_00034}.
FT ACT_SITE 7
FT /evidence="ECO:0000256|HAMAP-Rule:MF_00034"
FT ACT_SITE 68
FT /evidence="ECO:0000256|HAMAP-Rule:MF_00034"
FT ACT_SITE 141
FT /evidence="ECO:0000256|HAMAP-Rule:MF_00034"
FT BINDING 7
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="1"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_00034"
FT BINDING 68
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="2"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_00034"
FT BINDING 141
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="1"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_00034"
SQ SEQUENCE 188 AA; 19786 MW; 1D8619C9150406FB CRC64;
MRVMGVDPGL TRCGLSLIES GRGRQLTALD VDVVRTPSDA ALAQRLLAIS DAVEHWLDTH
HPEVVAIERV FSQLNVTTVM GTAQAGGVIA LAAAKRGVDV HFHTPSEVKA AVTGNGSADK
AQVTAMVTKI LALQAKPTPA DAADALALAI CHCWRAPTIA RMAEATSRAE ARAAQQRHAY
LAKLKAAR
//
