ID A0A059C3F5_EUCGR Unreviewed; 687 AA.
AC A0A059C3F5;
DT 09-JUL-2014, integrated into UniProtKB/TrEMBL.
DT 09-JUL-2014, sequence version 1.
DT 27-MAR-2024, entry version 45.
DE RecName: Full=ATP-dependent DNA helicase 2 subunit KU80 {ECO:0000256|PIRNR:PIRNR016570};
DE EC=3.6.4.12 {ECO:0000256|PIRNR:PIRNR016570};
GN ORFNames=EUGRSUZ_E01418 {ECO:0000313|EMBL:KCW72968.1};
OS Eucalyptus grandis (Flooded gum).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Myrtales; Myrtaceae; Myrtoideae; Eucalypteae; Eucalyptus.
OX NCBI_TaxID=71139 {ECO:0000313|EMBL:KCW72968.1};
RN [1] {ECO:0000313|EMBL:KCW72968.1}
RP NUCLEOTIDE SEQUENCE.
RC TISSUE=Leaf extractions {ECO:0000313|EMBL:KCW72968.1};
RA Schmutz J., Hayes R., Myburg A., Tuskan G., Grattapaglia D., Rokhsar D.S.;
RT "The genome of Eucalyptus grandis.";
RL Submitted (JUL-2013) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Single-stranded DNA-dependent ATP-dependent helicase.
CC {ECO:0000256|PIRNR:PIRNR016570}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.12;
CC Evidence={ECO:0000256|PIRNR:PIRNR016570};
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000256|ARBA:ARBA00004123,
CC ECO:0000256|PIRNR:PIRNR016570}.
CC -!- SIMILARITY: Belongs to the ku80 family. {ECO:0000256|ARBA:ARBA00007726,
CC ECO:0000256|PIRNR:PIRNR016570}.
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DR EMBL; KK198757; KCW72968.1; -; Genomic_DNA.
DR RefSeq; XP_010056303.1; XM_010058001.2.
DR AlphaFoldDB; A0A059C3F5; -.
DR STRING; 71139.A0A059C3F5; -.
DR EnsemblPlants; KCW72968; KCW72968; EUGRSUZ_E01418.
DR GeneID; 104444347; -.
DR Gramene; KCW72968; KCW72968; EUGRSUZ_E01418.
DR KEGG; egr:104444347; -.
DR eggNOG; KOG2326; Eukaryota.
DR InParanoid; A0A059C3F5; -.
DR OMA; WAMQYVW; -.
DR OrthoDB; 5884at2759; -.
DR GO; GO:0043564; C:Ku70:Ku80 complex; IBA:GO_Central.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR GO; GO:0003684; F:damaged DNA binding; IEA:InterPro.
DR GO; GO:0003678; F:DNA helicase activity; IEA:InterPro.
DR GO; GO:0042162; F:telomeric DNA binding; IBA:GO_Central.
DR GO; GO:0006310; P:DNA recombination; IEA:UniProtKB-KW.
DR GO; GO:0006303; P:double-strand break repair via nonhomologous end joining; IBA:GO_Central.
DR GO; GO:0000723; P:telomere maintenance; IBA:GO_Central.
DR CDD; cd00873; KU80; 1.
DR Gene3D; 1.10.1600.10; -; 1.
DR Gene3D; 2.40.290.10; -; 1.
DR Gene3D; 1.25.40.240; Ku, C-terminal domain; 1.
DR Gene3D; 3.40.50.410; von Willebrand factor, type A domain; 1.
DR InterPro; IPR006164; Ku70/Ku80_beta-barrel_dom.
DR InterPro; IPR024193; Ku80.
DR InterPro; IPR005160; Ku_C.
DR InterPro; IPR036494; Ku_C_sf.
DR InterPro; IPR005161; Ku_N.
DR InterPro; IPR014893; Ku_PK_bind.
DR InterPro; IPR016194; SPOC-like_C_dom_sf.
DR InterPro; IPR036465; vWFA_dom_sf.
DR PANTHER; PTHR12604; KU AUTOANTIGEN DNA HELICASE; 1.
DR PANTHER; PTHR12604:SF4; X-RAY REPAIR CROSS-COMPLEMENTING PROTEIN 5; 1.
DR Pfam; PF02735; Ku; 1.
DR Pfam; PF03730; Ku_C; 1.
DR Pfam; PF03731; Ku_N; 1.
DR Pfam; PF08785; Ku_PK_bind; 1.
DR PIRSF; PIRSF016570; Ku80; 1.
DR SMART; SM00559; Ku78; 1.
DR SUPFAM; SSF101420; C-terminal domain of Ku80; 1.
DR SUPFAM; SSF100939; SPOC domain-like; 1.
DR SUPFAM; SSF53300; vWA-like; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|PIRNR:PIRNR016570};
KW DNA damage {ECO:0000256|ARBA:ARBA00022763, ECO:0000256|PIRNR:PIRNR016570};
KW DNA recombination {ECO:0000256|ARBA:ARBA00023172,
KW ECO:0000256|PIRNR:PIRNR016570};
KW DNA repair {ECO:0000256|ARBA:ARBA00023204, ECO:0000256|PIRNR:PIRNR016570};
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125, ECO:0000256|PIRNR:PIRNR016570};
KW Helicase {ECO:0000256|ARBA:ARBA00022806, ECO:0000256|PIRNR:PIRNR016570};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|PIRNR:PIRNR016570};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741,
KW ECO:0000256|PIRNR:PIRNR016570};
KW Nucleus {ECO:0000256|ARBA:ARBA00023242, ECO:0000256|PIRNR:PIRNR016570}.
FT DOMAIN 278..419
FT /note="Ku"
FT /evidence="ECO:0000259|SMART:SM00559"
FT REGION 518..540
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 526..540
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 687 AA; 77874 MW; D2D804012AA115DE CRC64;
MARNKEALVL LLDVGPSMHH LLPEVEKICS MIVQKKLVYS KYDEVGVVLF GTEETENELT
MEVGGYEHVV VLQNIKVVDG DLVETLKQIP RGTVPGDFLD AIVVGMDMLI KKYSSTNKGK
KRLCLMTNGL SPIKDPYEGS KEDQVNAIAE QMTSHGMKLE CVVVRESVVR GENVSVMEEN
DRILNLFPQK SCAKTIFVES STSLLGALKT RNISPVTIFR GDLEISPKLK IKVWVYKKTS
EEKFPTLKKY SDKAPSTDKF ASHEVKVDYE YKSVEDANKV VPPEQRIKGY RYGPQVVPIS
ADEWDAVKFK PEKSVKLLGF TDSSNIMRHH YMKDVNIFIA DPGDQNAIVA VSALARAMKE
MHKVAILRCV WRQGQGNVVV GVLTPNVSDN DHVPDSFYFN ILPFAEDVRE FQFPSFSNLP
ASLLPDEEQQ EAADNFVKML DLTPSDRKEM LQPDFTPNPV LERFYNYLEL KSKHPDAAVP
PLDKTLKRIT EPDPELLAQK KSAIDSLLRH FEVKEKPKLK KSTRRMREKP SGSDDEDNYR
RISSKTEVEL IENGGKVEKI GDLTPVQDFE AMMSRRDNPE WVNRAITEMK NKIHDLIEDS
YEGDNYAKAL DLLVALRKGC ILEQEPKQFN DFLHQLCKFC LQNDLRSFCN FLATKGITLI
SKTEAADSDV TEEEARSFLV KSEPKPE
//
