ID A0A060HHD4_9ARCH Unreviewed; 196 AA.
AC A0A060HHD4;
DT 03-SEP-2014, integrated into UniProtKB/TrEMBL.
DT 03-SEP-2014, sequence version 1.
DT 24-JAN-2024, entry version 30.
DE RecName: Full=3-isopropylmalate dehydratase {ECO:0000256|ARBA:ARBA00011998};
DE EC=4.2.1.33 {ECO:0000256|ARBA:ARBA00011998};
GN Name=leuD1 {ECO:0000313|EMBL:AIC14958.1};
GN ORFNames=NVIE_007460 {ECO:0000313|EMBL:AIC14958.1};
OS Nitrososphaera viennensis EN76.
OC Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
OC Nitrososphaeraceae; Nitrososphaera.
OX NCBI_TaxID=926571 {ECO:0000313|EMBL:AIC14958.1, ECO:0000313|Proteomes:UP000027093};
RN [1] {ECO:0000313|EMBL:AIC14958.1, ECO:0000313|Proteomes:UP000027093}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=EN76 {ECO:0000313|EMBL:AIC14958.1};
RX PubMed=24907263; DOI=10.1099/ijs.0.063172-0;
RA Stieglmeier M., Klingl A., Alves R.J., Rittmann S.K., Melcher M.,
RA Leisch N., Schleper C.;
RT "Nitrososphaera viennensis gen. nov., sp. nov., an aerobic and mesophilic,
RT ammonia-oxidizing archaeon from soil and a member of the archaeal phylum
RT Thaumarchaeota.";
RL Int. J. Syst. Evol. Microbiol. 64:2738-2752(2014).
CC -!- FUNCTION: Catalyzes the isomerization between 2-isopropylmalate and 3-
CC isopropylmalate, via the formation of 2-isopropylmaleate.
CC {ECO:0000256|ARBA:ARBA00002695}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(2R,3S)-3-isopropylmalate = (2S)-2-isopropylmalate;
CC Xref=Rhea:RHEA:32287, ChEBI:CHEBI:1178, ChEBI:CHEBI:35121;
CC EC=4.2.1.33; Evidence={ECO:0000256|ARBA:ARBA00000491};
CC -!- PATHWAY: Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine
CC from 3-methyl-2-oxobutanoate: step 2/4.
CC {ECO:0000256|ARBA:ARBA00004729}.
CC -!- SUBUNIT: Heterodimer of LeuC and LeuD. {ECO:0000256|ARBA:ARBA00011271}.
CC -!- SIMILARITY: Belongs to the LeuD family. LeuD type 1 subfamily.
CC {ECO:0000256|ARBA:ARBA00009845}.
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DR EMBL; CP007536; AIC14958.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A060HHD4; -.
DR STRING; 926571.NVIE_007460; -.
DR KEGG; nvn:NVIE_007460; -.
DR HOGENOM; CLU_081378_0_3_2; -.
DR OrthoDB; 6505at2157; -.
DR UniPathway; UPA00048; UER00071.
DR Proteomes; UP000027093; Chromosome.
DR GO; GO:0009316; C:3-isopropylmalate dehydratase complex; IEA:InterPro.
DR GO; GO:0003861; F:3-isopropylmalate dehydratase activity; IEA:UniProtKB-EC.
DR GO; GO:0009098; P:leucine biosynthetic process; IEA:UniProtKB-UniPathway.
DR CDD; cd01577; IPMI_Swivel; 1.
DR Gene3D; 3.20.19.10; Aconitase, domain 4; 1.
DR HAMAP; MF_01031; LeuD_type1; 1.
DR InterPro; IPR004431; 3-IsopropMal_deHydase_ssu.
DR InterPro; IPR015928; Aconitase/3IPM_dehydase_swvl.
DR InterPro; IPR000573; AconitaseA/IPMdHydase_ssu_swvl.
DR InterPro; IPR033940; IPMI_Swivel.
DR NCBIfam; TIGR00171; leuD; 1.
DR PANTHER; PTHR43345:SF5; 3-ISOPROPYLMALATE DEHYDRATASE SMALL SUBUNIT; 1.
DR PANTHER; PTHR43345; 3-ISOPROPYLMALATE DEHYDRATASE SMALL SUBUNIT 2-RELATED-RELATED; 1.
DR Pfam; PF00694; Aconitase_C; 1.
DR SUPFAM; SSF52016; LeuD/IlvD-like; 1.
PE 3: Inferred from homology;
KW Amino-acid biosynthesis {ECO:0000256|ARBA:ARBA00022605};
KW Branched-chain amino acid biosynthesis {ECO:0000256|ARBA:ARBA00023304};
KW Leucine biosynthesis {ECO:0000256|ARBA:ARBA00022430};
KW Lyase {ECO:0000256|ARBA:ARBA00023239, ECO:0000313|EMBL:AIC14958.1};
KW Reference proteome {ECO:0000313|Proteomes:UP000027093}.
FT DOMAIN 1..123
FT /note="Aconitase A/isopropylmalate dehydratase small
FT subunit swivel"
FT /evidence="ECO:0000259|Pfam:PF00694"
SQ SEQUENCE 196 AA; 22463 MW; 06803031BB2D2F57 CRC64;
MEPFTVLKSK ATPLDRVNVD TDQIVPKQFL KLVSRTGFGQ YLFYDWRFDA EGRPRPGFVL
NDPRYKGRQI LLARDNFGSG SSREHAAWAI LDYGFRAVIA PSFADIFYNN CFKNGVLPVR
LAAKDVDYLF QNGDVEIEID LTKQVVKAGQ KTMHFDIDEF RKKLLLEGLD SIGLTLKLEN
EISMYERERQ AFAPSL
//
