ID A0A061EZT0_THECC Unreviewed; 1536 AA.
AC A0A061EZT0;
DT 03-SEP-2014, integrated into UniProtKB/TrEMBL.
DT 03-SEP-2014, sequence version 1.
DT 22-FEB-2023, entry version 37.
DE RecName: Full=HECT-type E3 ubiquitin transferase {ECO:0000256|ARBA:ARBA00012485};
DE EC=2.3.2.26 {ECO:0000256|ARBA:ARBA00012485};
GN ORFNames=TCM_022084 {ECO:0000313|EMBL:EOY07744.1};
OS Theobroma cacao (Cacao) (Cocoa).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Malvales; Malvaceae; Byttnerioideae; Theobroma.
OX NCBI_TaxID=3641 {ECO:0000313|EMBL:EOY07744.1, ECO:0000313|Proteomes:UP000026915};
RN [1] {ECO:0000313|EMBL:EOY07744.1, ECO:0000313|Proteomes:UP000026915}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Matina 1-6 {ECO:0000313|Proteomes:UP000026915};
RX PubMed=23731509; DOI=10.1186/gb-2013-14-6-r53;
RA Motamayor J.C., Mockaitis K., Schmutz J., Haiminen N., Iii D.L.,
RA Cornejo O., Findley S.D., Zheng P., Utro F., Royaert S., Saski C.,
RA Jenkins J., Podicheti R., Zhao M., Scheffler B.E., Stack J.C., Feltus F.A.,
RA Mustiga G.M., Amores F., Phillips W., Marelli J.P., May G.D., Shapiro H.,
RA Ma J., Bustamante C.D., Schnell R.J., Main D., Gilbert D., Parida L.,
RA Kuhn D.N.;
RT "The genome sequence of the most widely cultivated cacao type and its use
RT to identify candidate genes regulating pod color.";
RL Genome Biol. 14:R53.1-R53.24(2013).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine +
CC [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-
CC cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.;
CC EC=2.3.2.26; Evidence={ECO:0000256|ARBA:ARBA00000885};
CC -!- SIMILARITY: Belongs to the UPL family. K-HECT subfamily.
CC {ECO:0000256|ARBA:ARBA00006331}.
CC -!- CAUTION: Lacks conserved residue(s) required for the propagation of
CC feature annotation. {ECO:0000256|PROSITE-ProRule:PRU00104}.
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DR EMBL; CM001883; EOY07744.1; -; Genomic_DNA.
DR EnsemblPlants; EOY07744; EOY07744; TCM_022084.
DR Gramene; EOY07744; EOY07744; TCM_022084.
DR Proteomes; UP000026915; Chromosome 5.
DR GO; GO:0016874; F:ligase activity; IEA:UniProtKB-KW.
DR GO; GO:0061630; F:ubiquitin protein ligase activity; IEA:InterPro.
DR GO; GO:0006511; P:ubiquitin-dependent protein catabolic process; IEA:InterPro.
DR Gene3D; 3.30.2410.10; Hect, E3 ligase catalytic domain; 1.
DR Gene3D; 3.90.1750.10; Hect, E3 ligase catalytic domains; 1.
DR Gene3D; 1.25.10.10; Leucine-rich Repeat Variant; 1.
DR InterPro; IPR011989; ARM-like.
DR InterPro; IPR016024; ARM-type_fold.
DR InterPro; IPR000569; HECT_dom.
DR InterPro; IPR035983; Hect_E3_ubiquitin_ligase.
DR InterPro; IPR045322; HECTD1/TRIP12-like.
DR PANTHER; PTHR45670; E3 UBIQUITIN-PROTEIN LIGASE TRIP12; 1.
DR PANTHER; PTHR45670:SF10; E3 UBIQUITIN-PROTEIN LIGASE UPL4; 1.
DR Pfam; PF00632; HECT; 1.
DR SMART; SM00119; HECTc; 1.
DR SUPFAM; SSF48371; ARM repeat; 1.
DR SUPFAM; SSF56204; Hect, E3 ligase catalytic domain; 1.
DR PROSITE; PS50237; HECT; 1.
PE 3: Inferred from homology;
KW Ligase {ECO:0000313|EMBL:EOY07744.1};
KW Reference proteome {ECO:0000313|Proteomes:UP000026915};
KW Ubl conjugation pathway {ECO:0000256|ARBA:ARBA00022786,
KW ECO:0000256|PROSITE-ProRule:PRU00104}.
FT DOMAIN 1188..1525
FT /note="HECT"
FT /evidence="ECO:0000259|PROSITE:PS50237"
FT REGION 1..89
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 881..932
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 7..21
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 25..62
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 63..89
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1536 AA; 171837 MW; EF8D48879EB69AF5 CRC64;
MGNRGQKRTE TADELPADKR ACSSLEFRPS SSNSSSIQTH LNSPNSTPDA DMDTSSSASA
SSRSDGEHEK EEDSAYGSCD SEDAEQQPRH HILRDYQRRR SSSDHGKLNN ILSNLNEEGN
GDSGQLAALT ELCEVLSFCT EDSISSLMAD TLSPILVKLA KNESNANIML LAIRSITYLS
DVFPRSSGFL VRHDAVPALC QRLLAIEYVD VAEQCLQALE KISRDQPLAC LQAGAIMAVL
NFIDFFSISV QRVALSTVVN ICKKLPLEGP APFVEAVPKL CDLLQHEDQQ LVESVATCLI
KIAERMCQSS EMLEELCKHE LINQVTHLMN LNSRTTVSQP IYNGLIGLLV KLSSGSFVAF
RSLYELNISS ILKDLLSTYD LTHGMSSPHS VDGNCNQVHE VLKLLNELLP TSTGDLGNQL
LLDKKSFLAD HPDLLQKFGM DMLPMLVQVV NSGANIYVCY GCLSVISKLV FLSKSDMLVE
LLKTANIPSF LAGVFTRKDH HLLMLALQIV EMILQKLSDV FLNSFIKEGV FFAIDTLLMP
EKCSQVMLPV FSGFQSLFDS SQKSSARDIR RCLCYAFDTV PSSSAPPCKL DKDSVCNLAK
HIKTSYFAPE LSDSEKGMTD ILQNLRTFSA ALSDLINMPV DDDTPAQHEE KFHSILHQIM
LKLNGRERVS TFEFIESGIV KALMHYLSNG LYLRNNVEFN GIYNHLLVLG KRFEVFAKLF
LSYSDIPVED LPLSVLIQKL QSALSSLENF PVIPSHGFKQ KTSFATVPNG RCIMYPCFRV
RFVRGEGETC LSDCPEDILT VDPFSSSDAI EGYLWPKVFI KRTENGESDA EALEQMESQP
IHLPSNANSS QGESSGFIDS MSADLPEMQE DEANLSQFAS EEVHFRESNS GETMSLDETN
MGSTAQVQQF PTESTTKMKP QCSASGNNDN EDSSPRLLLY LEGHQLDRTL TLYQAILQQL
LNSENEFITW AKLWTRVYTL TYKKALESKQ DDAQEHTLLE QKSSISDKNV ASMQNMAFFS
SLFACKLASN LDKSSPAYDI LFLLKSLEGI NKCSFHLMSY ERIRAFAEGR IDNLDNLKVM
VHSVPQNEFV SSRLTEKLEQ QMRDSFTLST GGMPSWCNQL IASCPFLFSF EAKCKYFRLA
AFGPRRVQLH TTLRSNSGAS NDRQSTAAGL PRKKFLVWRD RILDSATRMM DLHARHKGLL
EVEYNEEVGT GLGPTLEFYT LVCHEFQKSG LGIWREDYRS IITSETLPVV DSGILINPYG
LFPHPWSPTT DSCNGIQFSE VLKKFVLLGQ IVAKAIQDGR VLDVPFSKAF YKIILGQDLC
LYDIQSFNPE LGRTLLEFQA IVDRKMHLES ICVENSTLKL DLCFRNTRIE DLCLDFTLPG
YPDYVLSSEC NHKMVNLANL DNYIKLVVDA TIHTGIARQV EAFKSGFNQV FAIKHLHIFT
GEELERLLCG ERDFWAFNEL LEHIKFDHGY TASSPPIINL LEIIQEFEYA QRRAFLQFVT
GAPRLPPGGL ASLNPKLTIV RKSIVAIVLI QSCPVL
//
