ID A0A061FR78_THECC Unreviewed; 1788 AA.
AC A0A061FR78;
DT 03-SEP-2014, integrated into UniProtKB/TrEMBL.
DT 03-SEP-2014, sequence version 1.
DT 27-MAR-2024, entry version 54.
DE RecName: Full=DNA-directed RNA polymerase subunit {ECO:0000256|RuleBase:RU004279};
DE EC=2.7.7.6 {ECO:0000256|RuleBase:RU004279};
GN ORFNames=TCM_045152 {ECO:0000313|EMBL:EOY19810.1};
OS Theobroma cacao (Cacao) (Cocoa).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Malvales; Malvaceae; Byttnerioideae; Theobroma.
OX NCBI_TaxID=3641 {ECO:0000313|EMBL:EOY19810.1, ECO:0000313|Proteomes:UP000026915};
RN [1] {ECO:0000313|EMBL:EOY19810.1, ECO:0000313|Proteomes:UP000026915}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Matina 1-6 {ECO:0000313|Proteomes:UP000026915};
RX PubMed=23731509; DOI=10.1186/gb-2013-14-6-r53;
RA Motamayor J.C., Mockaitis K., Schmutz J., Haiminen N., Iii D.L.,
RA Cornejo O., Findley S.D., Zheng P., Utro F., Royaert S., Saski C.,
RA Jenkins J., Podicheti R., Zhao M., Scheffler B.E., Stack J.C., Feltus F.A.,
RA Mustiga G.M., Amores F., Phillips W., Marelli J.P., May G.D., Shapiro H.,
RA Ma J., Bustamante C.D., Schnell R.J., Main D., Gilbert D., Parida L.,
RA Kuhn D.N.;
RT "The genome sequence of the most widely cultivated cacao type and its use
RT to identify candidate genes regulating pod color.";
RL Genome Biol. 14:R53.1-R53.24(2013).
CC -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC {ECO:0000256|RuleBase:RU004279}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.6; Evidence={ECO:0000256|RuleBase:RU004279};
CC -!- SIMILARITY: Belongs to the RNA polymerase beta' chain family.
CC {ECO:0000256|RuleBase:RU004279}.
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DR EMBL; CM001888; EOY19809.1; -; Genomic_DNA.
DR EMBL; CM001888; EOY19810.1; -; Genomic_DNA.
DR STRING; 3641.A0A061FR78; -.
DR EnsemblPlants; EOY19809; EOY19809; TCM_045152.
DR EnsemblPlants; EOY19810; EOY19810; TCM_045152.
DR Gramene; EOY19809; EOY19809; TCM_045152.
DR Gramene; EOY19810; EOY19810; TCM_045152.
DR eggNOG; KOG0260; Eukaryota.
DR eggNOG; KOG2992; Eukaryota.
DR HOGENOM; CLU_002449_2_0_1; -.
DR InParanoid; A0A061FR78; -.
DR OMA; WGSQQKS; -.
DR Proteomes; UP000026915; Chromosome 10.
DR GO; GO:0005665; C:RNA polymerase II, core complex; IBA:GO_Central.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-EC.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0006366; P:transcription by RNA polymerase II; IEA:GOC.
DR CDD; cd02737; RNAP_IV_NRPD1_C; 1.
DR Gene3D; 2.40.40.20; -; 1.
DR Gene3D; 3.10.450.40; -; 1.
DR Gene3D; 6.20.50.80; -; 1.
DR Gene3D; 3.30.1490.180; RNA polymerase ii; 1.
DR Gene3D; 4.10.860.120; RNA polymerase II, clamp domain; 1.
DR Gene3D; 1.10.274.100; RNA polymerase Rpb1, domain 3; 1.
DR InterPro; IPR045867; DNA-dir_RpoC_beta_prime.
DR InterPro; IPR006594; LisH.
DR InterPro; IPR040402; NRPD1_C.
DR InterPro; IPR000722; RNA_pol_asu.
DR InterPro; IPR006592; RNA_pol_N.
DR InterPro; IPR007080; RNA_pol_Rpb1_1.
DR InterPro; IPR007066; RNA_pol_Rpb1_3.
DR InterPro; IPR042102; RNA_pol_Rpb1_3_sf.
DR InterPro; IPR007081; RNA_pol_Rpb1_5.
DR InterPro; IPR044893; RNA_pol_Rpb1_clamp_domain.
DR PANTHER; PTHR19376; DNA-DIRECTED RNA POLYMERASE; 1.
DR PANTHER; PTHR19376:SF51; DNA-DIRECTED RNA POLYMERASE V SUBUNIT 1; 1.
DR Pfam; PF11523; DUF3223; 1.
DR Pfam; PF04997; RNA_pol_Rpb1_1; 1.
DR Pfam; PF00623; RNA_pol_Rpb1_2; 1.
DR Pfam; PF04983; RNA_pol_Rpb1_3; 1.
DR Pfam; PF04998; RNA_pol_Rpb1_5; 1.
DR SMART; SM00663; RPOLA_N; 1.
DR SUPFAM; SSF64484; beta and beta-prime subunits of DNA dependent RNA-polymerase; 1.
DR PROSITE; PS50896; LISH; 1.
PE 3: Inferred from homology;
KW DNA-directed RNA polymerase {ECO:0000256|ARBA:ARBA00022478,
KW ECO:0000256|RuleBase:RU004279}; Magnesium {ECO:0000256|ARBA:ARBA00022842};
KW Nucleotidyltransferase {ECO:0000256|ARBA:ARBA00022695,
KW ECO:0000256|RuleBase:RU004279};
KW Reference proteome {ECO:0000313|Proteomes:UP000026915};
KW Transcription {ECO:0000256|ARBA:ARBA00023163,
KW ECO:0000256|RuleBase:RU004279};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|RuleBase:RU004279};
KW Zinc {ECO:0000256|ARBA:ARBA00022833}.
FT DOMAIN 205..504
FT /note="RNA polymerase N-terminal"
FT /evidence="ECO:0000259|SMART:SM00663"
FT REGION 1458..1477
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1492..1631
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1504..1521
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1542..1611
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1612..1626
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1788 AA; 198799 MW; 182D0CA71CE284FD CRC64;
MEENSSASTV DGEIVGIGFC LATPREIFTA SISGFPINHV SQLSNSYLGL PLEFGKCNAC
GTSEPGKCEG HFGYIELPIP IYHPSHISEL KRLLSLLCLK CLRMKNKFQI KSGSISDRLL
ASCCENAPQV SIKEVKTTDG ACSLELKQPS RQARTSWEFL EKYGFRYGDH HNTRTLLPCE
VMEILKRIPA ETRRKLSGKG FFPQEGYILR YLPVPPNCLS VPDISDGVSI MSSDLSTAML
KKVLKQVEII KSSRSGTPNF ESHEVEANDL QSAVEQYLQV RGTVKASRNI DARYGISKDA
SDSSTKAWLE KMRTLFIRKG SGFSSRGVIT GDPYKKVNEI GIPSEIAQRI TFEERVNMHN
MRYLQNLVDN KLCLTYRDGS STYSLREGSK GHTFLRPGQV VHRRIMDGDI VFINRPPTTH
KHSLQALSVY VHDDHTVKIN PLICGPLSAD FDGDCIHLFY PQSLAAKAEV FELFSVEKQL
LSSHNGNLNL QLATDSLLSL RVMLKTLLFK KADAQQLSMF LSSALPQPAF LKGNSFGPCW
TALQILQTAF PACLDCSGDR YLISKSDILT VDFSRDLMQS VINEVVTSIF FEKGPKEVLN
FFDSLQPLLM ENVFAEGFSV SLEDFSVSRE VIQNIQKDIQ DISPLLYQLR STYNELVGLQ
MENHIRVAKA PVANFILNSS ALGDLIDSKS DSTVNKVVQQ IGFLGLQLSN KGKFYSKTLV
EDVAYQFQSI YPSDGVDYPS AEFGLIKSCF FHGLDPYEGM VHSISTREVI VRSSRGLSEP
GTLFKNLMAI LRDVVICYDG TVRNISSNSI IQFQYGLNAR TKPQFPAGEP VGVLAATAMS
NPAYKAVLDS TPSSNSSWEL MKEILLCKVS LKNDLVDRRV ILYLKDCDCG RKYCQENAAY
LVKNHLRKVK LKDTAVELIF EYKQQQTVSE SEAGLVGHIL LNKAVLKELN ISMQEVHMKC
QETIISFRKK KKTADTFKRT DLFFSECCSI QQSCGGKWLD MSCLMFFCRN TKDDHLDCTL
QDLVDIIYPV LLETVIKGDP RICSANIIWV SPDTTTWIRS PSKTQKGELA LDVVLEKSAV
KQNGDAWRTV IDCCLPVINL IDTQRSIPYA IKQVQELLGI SCAFEQAVQR LSTSVSMVAR
GVLKEHLILL ANSMTCAGNL IGFNSGGYKA LSRSLNIQVP FSEATLFTPR KCFERAAEKC
HVDSLSSIVA SCSWGKHVAV GTGSRFDVLW DRKEVGFDQK SGIDVYNFLH MLSSASGPSS
TTTCLGEEVD DLMDVDNMAE WSLSPEHSNG LDKPVFEDAA DFENDLDFQP AESSWEKGVS
LDKVSSWNVS SAWNKKAEDG DKFAAALTST TKQSDWCDWG TSKSKTQDAA AAATSTTKKT
EWCDWGTSKS KTQEVAATVT GTAEQNEWCD WRTSKSKIQV VAAAVTSTTK QSEWGDWGTS
KSKTQDVAAA VTGTMETEWG DWGKGKSKTQ DVSPKVDGTC VNEQTKLSDW GLKKNDTQDV
SMEEKTFKSN GADTGTSWGT MGKESEKPDA NDALPWSGWG TQDVIPTKTL DDSSKSSGWE
QQKSPECSQG WGSLDESNQP ASSNGWDTPN GLGSTQSEKQ HQWGQSRGSR RWASDASKKN
HPVKSARVMN DDSSMAAMYT ATRQRLDMFT SEEQDILSDV EPLMQSIRKI MHQSGYNDGD
PLSALDQSFI LENVFTHHPD KAIKMGAGVD YVMVSKHSNF PDSRCFYVVS TDGRKQDFSY
RKCLDNFIKG KYPDMADVFI AKYFRKPRFG GFRERSVAPE NTEGENRK
//
