ID A0A061FVB2_THECC Unreviewed; 559 AA.
AC A0A061FVB2;
DT 03-SEP-2014, integrated into UniProtKB/TrEMBL.
DT 03-SEP-2014, sequence version 1.
DT 27-MAR-2024, entry version 41.
DE RecName: Full=apyrase {ECO:0000256|ARBA:ARBA00012148};
DE EC=3.6.1.5 {ECO:0000256|ARBA:ARBA00012148};
DE AltName: Full=ATP-diphosphatase {ECO:0000256|ARBA:ARBA00031428};
DE AltName: Full=ATP-diphosphohydrolase {ECO:0000256|ARBA:ARBA00032306};
DE AltName: Full=Adenosine diphosphatase {ECO:0000256|ARBA:ARBA00030084};
DE AltName: Full=NTPDase {ECO:0000256|ARBA:ARBA00031370};
GN ORFNames=TCM_012154 {ECO:0000313|EMBL:EOY20807.1};
OS Theobroma cacao (Cacao) (Cocoa).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Malvales; Malvaceae; Byttnerioideae; Theobroma.
OX NCBI_TaxID=3641 {ECO:0000313|EMBL:EOY20807.1, ECO:0000313|Proteomes:UP000026915};
RN [1] {ECO:0000313|EMBL:EOY20807.1, ECO:0000313|Proteomes:UP000026915}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Matina 1-6 {ECO:0000313|Proteomes:UP000026915};
RX PubMed=23731509; DOI=10.1186/gb-2013-14-6-r53;
RA Motamayor J.C., Mockaitis K., Schmutz J., Haiminen N., Iii D.L.,
RA Cornejo O., Findley S.D., Zheng P., Utro F., Royaert S., Saski C.,
RA Jenkins J., Podicheti R., Zhao M., Scheffler B.E., Stack J.C., Feltus F.A.,
RA Mustiga G.M., Amores F., Phillips W., Marelli J.P., May G.D., Shapiro H.,
RA Ma J., Bustamante C.D., Schnell R.J., Main D., Gilbert D., Parida L.,
RA Kuhn D.N.;
RT "The genome sequence of the most widely cultivated cacao type and its use
RT to identify candidate genes regulating pod color.";
RL Genome Biol. 14:R53.1-R53.24(2013).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + 2 H2O = a ribonucleoside
CC 5'-phosphate + 2 H(+) + 2 phosphate; Xref=Rhea:RHEA:36795,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:43474,
CC ChEBI:CHEBI:58043, ChEBI:CHEBI:61557; EC=3.6.1.5;
CC Evidence={ECO:0000256|ARBA:ARBA00000211};
CC -!- SIMILARITY: Belongs to the GDA1/CD39 NTPase family.
CC {ECO:0000256|ARBA:ARBA00009283}.
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DR EMBL; CM001881; EOY20807.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A061FVB2; -.
DR EnsemblPlants; EOY20807; EOY20807; TCM_012154.
DR Gramene; EOY20807; EOY20807; TCM_012154.
DR eggNOG; KOG1386; Eukaryota.
DR HOGENOM; CLU_010246_2_2_1; -.
DR InParanoid; A0A061FVB2; -.
DR OMA; TVNTTIW; -.
DR Proteomes; UP000026915; Chromosome 3.
DR GO; GO:0016020; C:membrane; IBA:GO_Central.
DR GO; GO:0004050; F:apyrase activity; IEA:UniProtKB-EC.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0017110; F:nucleoside diphosphate phosphatase activity; IBA:GO_Central.
DR GO; GO:0009134; P:nucleoside diphosphate catabolic process; IBA:GO_Central.
DR Gene3D; 3.30.420.40; -; 1.
DR Gene3D; 3.30.420.150; Exopolyphosphatase. Domain 2; 1.
DR InterPro; IPR000407; GDA1_CD39_NTPase.
DR PANTHER; PTHR11782; ADENOSINE/GUANOSINE DIPHOSPHATASE; 1.
DR PANTHER; PTHR11782:SF92; APYRASE; 1.
DR Pfam; PF01150; GDA1_CD39; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|PIRSR:PIRSR600407-2};
KW Membrane {ECO:0000256|SAM:Phobius};
KW Nucleotide-binding {ECO:0000256|PIRSR:PIRSR600407-2};
KW Reference proteome {ECO:0000313|Proteomes:UP000026915};
KW Transmembrane {ECO:0000256|SAM:Phobius};
KW Transmembrane helix {ECO:0000256|SAM:Phobius}.
FT TRANSMEM 21..43
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 510..530
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT ACT_SITE 199
FT /note="Proton acceptor"
FT /evidence="ECO:0000256|PIRSR:PIRSR600407-1"
FT BINDING 230..234
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000256|PIRSR:PIRSR600407-2"
SQ SEQUENCE 559 AA; 61894 MW; 392D57C0D6157B4E CRC64;
MEPKSPSKVK LSVMGFKRWK RVLKVFTFGF LILLSCIGVY LAFNFVKAWN VLESSYYTVV
VDCGSTGTRV NVFEWEKGGL VNQGLPYLVH SYPDYSTKSP LWRNSCHYHC MQTEPGLDKF
VGNASGMRAS LEPLIAWAEQ MVPHERHGDT PIIVLATAGL RRLAAKDARQ VLDDVEIVVR
GHSFVYSKNW IRVLTGKEEA YYGWVALNYK LGSLGNSLKA STFGLLDLGG SSLQVVVEVA
EENGNENVMT SNIGSTDHNI LAYSLPAFGL TEAFDRTVVM LSQNQTDRGN TTNRFEVRHP
CLSSDFVQNY TCSGCPMPNV TDLENSESLM YKSQFSLTYL VGDPNWEQCK ELVRAAAMNY
SGSDWSHQIV GRNCEANSSP YGGSNMLNLT AVAHHSGRFH ALSGFFVVND MLHLSPRASV
TEIWEKGEQL CSRSSTELSS ISQRQTYAGQ SCFRVPYVAS LIEDSLCLGN GEIVFGPGDV
SWTLGAALIH GLDSIETPAS ISTTKDMDIF SSPVFLFVLL LFLLFVVYCS QITLPMLGRK
VADVGVSLPS YVHPRRRQT
//
