ID A0A061J2U7_TRYRA Unreviewed; 655 AA.
AC A0A061J2U7;
DT 03-SEP-2014, integrated into UniProtKB/TrEMBL.
DT 03-SEP-2014, sequence version 1.
DT 27-MAR-2024, entry version 38.
DE RecName: Full=Lysine--tRNA ligase {ECO:0000256|ARBA:ARBA00013166, ECO:0000256|RuleBase:RU003748};
DE EC=6.1.1.6 {ECO:0000256|ARBA:ARBA00013166, ECO:0000256|RuleBase:RU003748};
DE AltName: Full=Lysyl-tRNA synthetase {ECO:0000256|ARBA:ARBA00030563, ECO:0000256|RuleBase:RU003748};
GN ORFNames=TRSC58_02512 {ECO:0000313|EMBL:ESL09763.1};
OS Trypanosoma rangeli SC58.
OC Eukaryota; Discoba; Euglenozoa; Kinetoplastea; Metakinetoplastina;
OC Trypanosomatida; Trypanosomatidae; Trypanosoma; Herpetosoma.
OX NCBI_TaxID=429131 {ECO:0000313|EMBL:ESL09763.1, ECO:0000313|Proteomes:UP000031737};
RN [1] {ECO:0000313|EMBL:ESL09763.1, ECO:0000313|Proteomes:UP000031737}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=SC58 {ECO:0000313|EMBL:ESL09763.1,
RC ECO:0000313|Proteomes:UP000031737};
RA Stoco P.H., Wagner G., Gerber A., Zaha A., Thompson C., Bartholomeu D.C.,
RA Luckemeyer D.D., Bahia D., Loreto E., Prestes E.B., Lima F.M.,
RA Rodrigues-Luiz G., Vallejo G.A., Filho J.F., Monteiro K.M., Tyler K.M.,
RA de Almeida L.G., Ortiz M.F., Siervo M.A., de Moraes M.H., Cunha O.L.,
RA Mendonca-Neto R., Silva R., Teixeira S.M., Murta S.M., Sincero T.C.,
RA Mendes T.A., Urmenyi T.P., Silva V.G., da Rocha W.D., Andersson B.,
RA Romanha A.J., Steindel M., de Vasconcelos A.T., Grisard E.C.;
RL Submitted (JUL-2013) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + L-lysine + tRNA(Lys) = AMP + diphosphate + L-lysyl-
CC tRNA(Lys); Xref=Rhea:RHEA:20792, Rhea:RHEA-COMP:9696, Rhea:RHEA-
CC COMP:9697, ChEBI:CHEBI:30616, ChEBI:CHEBI:32551, ChEBI:CHEBI:33019,
CC ChEBI:CHEBI:78442, ChEBI:CHEBI:78529, ChEBI:CHEBI:456215; EC=6.1.1.6;
CC Evidence={ECO:0000256|ARBA:ARBA00000204,
CC ECO:0000256|RuleBase:RU003748};
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:ESL09763.1}.
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DR EMBL; AUPL01002512; ESL09763.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A061J2U7; -.
DR EnsemblProtists; ESL09763; ESL09763; TRSC58_02512.
DR VEuPathDB; TriTrypDB:TRSC58_02512; -.
DR OrthoDB; 168081at2759; -.
DR Proteomes; UP000031737; Unassembled WGS sequence.
DR GO; GO:0005737; C:cytoplasm; IEA:InterPro.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0004824; F:lysine-tRNA ligase activity; IEA:UniProtKB-EC.
DR GO; GO:0003676; F:nucleic acid binding; IEA:InterPro.
DR GO; GO:0006430; P:lysyl-tRNA aminoacylation; IEA:InterPro.
DR CDD; cd00775; LysRS_core; 1.
DR Gene3D; 2.40.50.140; Nucleic acid-binding proteins; 1.
DR HAMAP; MF_00252; Lys_tRNA_synth_class2; 1.
DR InterPro; IPR004364; Aa-tRNA-synt_II.
DR InterPro; IPR006195; aa-tRNA-synth_II.
DR InterPro; IPR045864; aa-tRNA-synth_II/BPL/LPL.
DR InterPro; IPR002313; Lys-tRNA-ligase_II.
DR InterPro; IPR018149; Lys-tRNA-synth_II_C.
DR InterPro; IPR012340; NA-bd_OB-fold.
DR InterPro; IPR004365; NA-bd_OB_tRNA.
DR NCBIfam; TIGR00499; lysS_bact; 1.
DR PANTHER; PTHR42918:SF1; LYSINE--TRNA LIGASE; 1.
DR PANTHER; PTHR42918; LYSYL-TRNA SYNTHETASE; 1.
DR Pfam; PF00152; tRNA-synt_2; 1.
DR Pfam; PF01336; tRNA_anti-codon; 1.
DR PRINTS; PR00982; TRNASYNTHLYS.
DR SUPFAM; SSF55681; Class II aaRS and biotin synthetases; 1.
DR SUPFAM; SSF50249; Nucleic acid-binding proteins; 1.
DR PROSITE; PS50862; AA_TRNA_LIGASE_II; 1.
PE 3: Inferred from homology;
KW Aminoacyl-tRNA synthetase {ECO:0000313|EMBL:ESL09763.1};
KW Ligase {ECO:0000313|EMBL:ESL09763.1};
KW Reference proteome {ECO:0000313|Proteomes:UP000031737}.
FT DOMAIN 234..556
FT /note="Aminoacyl-transfer RNA synthetases class-II family
FT profile"
FT /evidence="ECO:0000259|PROSITE:PS50862"
FT REGION 28..48
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 655 AA; 74039 MW; 04A544DDD0356E8C CRC64;
MRRWGAYSLQ CPCVCLLSVS KRAANVSPPP TASAFSPSSS ALKPQKLKNK TCRTKTVTQE
ASRAPRTRLD MPAAYSSFRD IAPIDVVREQ YDYLSPGEWA AQALVRVAGR VTSLRDMGKI
LFLTISSNAR ELQVVGQVGE HFTHEELKKL KVLLWVGDII GVEGVPCRMQ RGELSVAASR
ILVLSPYVCT DQVVCPNLRG FAVMQDNDVK YRYRFVDMMT NPCVISTIKK RHVMLQALRE
YLNERNFVEV ETPVLHTVAS GANAKPFVTH HNANGMDLFL RVAPELHLKQ CIVGGMERVY
EIGKVFRNED ADRTHNPEFT SCEFYAAYHT YEDLMPMTED IFRRLATRAN GTTVVQLPLD
GAHDGPLVEV DLSKPFRRVS VYDEVQRVSG VELPPPSELN TSKGIAYMSV LMLRHNISLP
PVRTAAKLFD KLIGFFITDR IVEPTFVMDH PLFMSPLAKE HASRPGLAER FELFINGMEY
CNAYSELNDP QEQYQRFQQQ LVDRQGGDEE AMTLDETFLK SLQVGLPPTA GWGMGIDRAL
MLLTNSSTIR DGIIFPLLRE DVRSHDSKRR HKTASFFEFN KQMTLFCLSG LEQELKRSGL
SEDACAHLRE LRKTIMGLGQ RSGTDGLPMT RDRLPEWRLT LMLAIIRFMC GAHRR
//
