ID   A0A067D587_SAPPC        Unreviewed;       554 AA.
AC   A0A067D587;
DT   03-SEP-2014, integrated into UniProtKB/TrEMBL.
DT   03-SEP-2014, sequence version 1.
DT   27-MAR-2024, entry version 41.
DE   RecName: Full=methionine--tRNA ligase {ECO:0000256|ARBA:ARBA00012838};
DE            EC=6.1.1.10 {ECO:0000256|ARBA:ARBA00012838};
GN   ORFNames=SPRG_01414 {ECO:0000313|EMBL:KDO34157.1};
OS   Saprolegnia parasitica (strain CBS 223.65).
OC   Eukaryota; Sar; Stramenopiles; Oomycota; Saprolegniales; Saprolegniaceae;
OC   Saprolegnia.
OX   NCBI_TaxID=695850 {ECO:0000313|EMBL:KDO34157.1, ECO:0000313|Proteomes:UP000030745};
RN   [1] {ECO:0000313|EMBL:KDO34157.1, ECO:0000313|Proteomes:UP000030745}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CBS 223.65 {ECO:0000313|EMBL:KDO34157.1,
RC   ECO:0000313|Proteomes:UP000030745};
RX   PubMed=23785293;
RA   Jiang R.H., de Bruijn I., Haas B.J., Belmonte R., Lobach L., Christie J.,
RA   van den Ackerveken G., Bottin A., Bulone V., Diaz-Moreno S.M., Dumas B.,
RA   Fan L., Gaulin E., Govers F., Grenville-Briggs L.J., Horner N.R.,
RA   Levin J.Z., Mammella M., Meijer H.J., Morris P., Nusbaum C., Oome S.,
RA   Phillips A.J., van Rooyen D., Rzeszutek E., Saraiva M., Secombes C.J.,
RA   Seidl M.F., Snel B., Stassen J.H., Sykes S., Tripathy S., van den Berg H.,
RA   Vega-Arreguin J.C., Wawra S., Young S.K., Zeng Q., Dieguez-Uribeondo J.,
RA   Russ C., Tyler B.M., van West P.;
RT   "Distinctive expansion of potential virulence genes in the genome of the
RT   oomycete fish pathogen Saprolegnia parasitica.";
RL   PLoS Genet. 9:E1003272-E1003272(2013).
CC   -!- SIMILARITY: Belongs to the class-I aminoacyl-tRNA synthetase family.
CC       {ECO:0000256|RuleBase:RU363039}.
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DR   EMBL; KK583191; KDO34157.1; -; Genomic_DNA.
DR   RefSeq; XP_012195021.1; XM_012339631.1.
DR   AlphaFoldDB; A0A067D587; -.
DR   STRING; 695850.A0A067D587; -.
DR   EnsemblProtists; KDO34157; KDO34157; SPRG_01414.
DR   GeneID; 24124007; -.
DR   KEGG; spar:SPRG_01414; -.
DR   VEuPathDB; FungiDB:SPRG_01414; -.
DR   OMA; NMFLPDR; -.
DR   OrthoDB; 166496at2759; -.
DR   Proteomes; UP000030745; Unassembled WGS sequence.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0004825; F:methionine-tRNA ligase activity; IEA:UniProtKB-EC.
DR   GO; GO:0006431; P:methionyl-tRNA aminoacylation; IEA:InterPro.
DR   CDD; cd00814; MetRS_core; 1.
DR   Gene3D; 2.170.220.10; -; 1.
DR   Gene3D; 3.40.50.620; HUPs; 1.
DR   InterPro; IPR014758; Met-tRNA_synth.
DR   InterPro; IPR023457; Met-tRNA_synth_2.
DR   InterPro; IPR015413; Methionyl/Leucyl_tRNA_Synth.
DR   InterPro; IPR033911; MetRS_core.
DR   InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR   InterPro; IPR009080; tRNAsynth_Ia_anticodon-bd.
DR   NCBIfam; TIGR00398; metG; 1.
DR   PANTHER; PTHR43326:SF1; METHIONINE--TRNA LIGASE, MITOCHONDRIAL; 1.
DR   PANTHER; PTHR43326; METHIONYL-TRNA SYNTHETASE; 1.
DR   Pfam; PF09334; tRNA-synt_1g; 1.
DR   PRINTS; PR01041; TRNASYNTHMET.
DR   SUPFAM; SSF47323; Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases; 1.
DR   SUPFAM; SSF52374; Nucleotidylyl transferase; 1.
PE   3: Inferred from homology;
KW   Aminoacyl-tRNA synthetase {ECO:0000256|ARBA:ARBA00023146,
KW   ECO:0000256|RuleBase:RU363039};
KW   ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|RuleBase:RU363039};
KW   Ligase {ECO:0000256|ARBA:ARBA00022598, ECO:0000256|RuleBase:RU363039};
KW   Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741,
KW   ECO:0000256|RuleBase:RU363039};
KW   Protein biosynthesis {ECO:0000256|ARBA:ARBA00022917,
KW   ECO:0000256|RuleBase:RU363039};
KW   Reference proteome {ECO:0000313|Proteomes:UP000030745}.
FT   DOMAIN          33..391
FT                   /note="Methionyl/Leucyl tRNA synthetase"
FT                   /evidence="ECO:0000259|Pfam:PF09334"
SQ   SEQUENCE   554 AA;  61827 MW;  823F96BAED8A7303 CRC64;
     MWRRSFAATA SMVPGNVQAR PRYLQSRLAT TKTITTPIYY VNAAPHLGHL HSTVMADALS
     RWFQFAGHKT LFSTGTDEHG LKVQQAAERA GKPTAAFCDD VATTFQAMCA RGDVAYDRFV
     RTTEPEHKAT VQSLWKTLVA NDAIYLGQHE AWYCVSDESF LTEMQVEENA DGKMVSKESG
     HPVELVKEEN YKFRLSAYQD ALLTWLDANP DVVQPKSRFN EVRSMVAGGL HDVSVSRLRE
     KIQWAIPVPD DENHSVYVWL DALSNYLTCA GYPDPEKLAE NWPASYHIVG KDIIKFHAIY
     WPAFLLAANL ALPKRIVAHA HWTVDNVKMS KSLGNVVEPN AIINTFGVDA VRYFLLREGV
     LTDDGDFNEE LLKNRVNSEV ADTLGNLVIR STTLAFVPNG IVPTGADYSA SDLELIAAMD
     AMVDATQAYY EKPDFSMAIR SVMFFLYDIN RNFSNNEPWV VAKELKDPSG LTTDEIVAKQ
     RFLDNTMLLS MEAARLSALL LLPVIPSTAT GILDYLNVPH AQRTLAHAKF GQSTYGPIRN
     AKAKVKFVPF QKLP
//