ID A0A067F6F6_CITSI Unreviewed; 266 AA.
AC A0A067F6F6;
DT 03-SEP-2014, integrated into UniProtKB/TrEMBL.
DT 03-SEP-2014, sequence version 1.
DT 27-MAR-2024, entry version 24.
DE RecName: Full=stizolobate synthase {ECO:0000256|ARBA:ARBA00013224};
DE EC=1.13.11.29 {ECO:0000256|ARBA:ARBA00013224};
GN ORFNames=CISIN_1g024528mg {ECO:0000313|EMBL:KDO58766.1};
OS Citrus sinensis (Sweet orange) (Citrus aurantium var. sinensis).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Sapindales; Rutaceae; Aurantioideae; Citrus.
OX NCBI_TaxID=2711 {ECO:0000313|EMBL:KDO58766.1, ECO:0000313|Proteomes:UP000027120};
RN [1] {ECO:0000313|EMBL:KDO58766.1, ECO:0000313|Proteomes:UP000027120}
RP NUCLEOTIDE SEQUENCE.
RG International Citrus Genome Consortium;
RA Gmitter F., Chen C., Farmerie W., Harkins T., Desany B., Mohiuddin M.,
RA Kodira C., Borodovsky M., Lomsadze A., Burns P., Jenkins J., Prochnik S.,
RA Shu S., Chapman J., Pitluck S., Schmutz J., Rokhsar D.;
RL Submitted (APR-2014) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=L-dopa + O2 = 4-(L-alanin-3-yl)-2-hydroxy-cis,cis-muconate 6-
CC semialdehyde + H(+); Xref=Rhea:RHEA:21220, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:15379, ChEBI:CHEBI:57504, ChEBI:CHEBI:57639;
CC EC=1.13.11.29; Evidence={ECO:0000256|ARBA:ARBA00000466};
CC -!- COFACTOR:
CC Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
CC Evidence={ECO:0000256|ARBA:ARBA00001947};
CC -!- PATHWAY: Pigment biosynthesis; betalain biosynthesis.
CC {ECO:0000256|ARBA:ARBA00005034}.
CC -!- SIMILARITY: Belongs to the DODA-type extradiol aromatic ring-opening
CC dioxygenase family. {ECO:0000256|ARBA:ARBA00007581}.
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DR EMBL; KK784946; KDO58766.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A067F6F6; -.
DR STRING; 2711.A0A067F6F6; -.
DR PaxDb; 2711-XP_006466223-1; -.
DR eggNOG; ENOG502QS66; Eukaryota.
DR Proteomes; UP000027120; Unassembled WGS sequence.
DR GO; GO:0051213; F:dioxygenase activity; IEA:UniProt.
DR GO; GO:0008198; F:ferrous iron binding; IEA:InterPro.
DR GO; GO:0016701; F:oxidoreductase activity, acting on single donors with incorporation of molecular oxygen; IEA:InterPro.
DR GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR GO; GO:0006725; P:cellular aromatic compound metabolic process; IEA:InterPro.
DR CDD; cd07363; 45_DOPA_Dioxygenase; 1.
DR Gene3D; 3.40.830.10; LigB-like; 1.
DR InterPro; IPR014436; Extradiol_dOase_DODA.
DR InterPro; IPR004183; Xdiol_dOase_suB.
DR PANTHER; PTHR30096:SF21; STIZOLOBATE SYNTHASE; 1.
DR PANTHER; PTHR30096; UNCHARACTERIZED; 1.
DR Pfam; PF02900; LigB; 1.
DR PIRSF; PIRSF006157; Doxgns_DODA; 1.
DR SUPFAM; SSF53213; LigB-like; 1.
PE 3: Inferred from homology;
KW Dioxygenase {ECO:0000256|ARBA:ARBA00022964};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Oxidoreductase {ECO:0000256|ARBA:ARBA00023002};
KW Reference proteome {ECO:0000313|Proteomes:UP000027120};
KW Zinc {ECO:0000256|ARBA:ARBA00022833}.
FT DOMAIN 7..262
FT /note="Extradiol ring-cleavage dioxygenase class III enzyme
FT subunit B"
FT /evidence="ECO:0000259|Pfam:PF02900"
SQ SEQUENCE 266 AA; 29900 MW; 0EC0CAF7F76FEA23 CRC64;
MSTVKNTFFI SHGSPTLSID ESLPARHFLK AWKEQVFTQR PNSILVISGH WETDVPTVNV
VQQNDVIYDF YNFPEQMYKL KYPAPGAPEL AKRVKQLLTE SGFNHVNEDT KRGLDHGAWV
PLMLMYPEAN IPVCQLSVQT RKDGTHHYNM GKALAPLKDE GVLIIGSGSA THNLRALQFD
GDSVASWASE FDNWLKDALL QGRYEDVNHY EQKAPCAKKA HPWPDHFYPL HVAMGAAGEN
ANAELIHTSW QLGTISYSSY KFTAAD
//
