ID A0A067P0S9_PLEOS Unreviewed; 1713 AA.
AC A0A067P0S9;
DT 03-SEP-2014, integrated into UniProtKB/TrEMBL.
DT 03-SEP-2014, sequence version 1.
DT 24-JAN-2024, entry version 39.
DE RecName: Full=DNA-directed RNA polymerase subunit {ECO:0000256|RuleBase:RU004279};
DE EC=2.7.7.6 {ECO:0000256|RuleBase:RU004279};
GN ORFNames=PLEOSDRAFT_1064266 {ECO:0000313|EMBL:KDQ29476.1};
OS Pleurotus ostreatus PC15.
OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes;
OC Agaricomycetidae; Agaricales; Pleurotineae; Pleurotaceae; Pleurotus.
OX NCBI_TaxID=1137138 {ECO:0000313|EMBL:KDQ29476.1, ECO:0000313|Proteomes:UP000027073};
RN [1] {ECO:0000313|Proteomes:UP000027073}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=PC15 {ECO:0000313|Proteomes:UP000027073};
RX PubMed=24958869; DOI=10.1073/pnas.1400592111;
RA Riley R., Salamov A.A., Brown D.W., Nagy L.G., Floudas D., Held B.W.,
RA Levasseur A., Lombard V., Morin E., Otillar R., Lindquist E.A., Sun H.,
RA LaButti K.M., Schmutz J., Jabbour D., Luo H., Baker S.E., Pisabarro A.G.,
RA Walton J.D., Blanchette R.A., Henrissat B., Martin F., Cullen D.,
RA Hibbett D.S., Grigoriev I.V.;
RT "Extensive sampling of basidiomycete genomes demonstrates inadequacy of the
RT white-rot/brown-rot paradigm for wood decay fungi.";
RL Proc. Natl. Acad. Sci. U.S.A. 111:9923-9928(2014).
CC -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC {ECO:0000256|RuleBase:RU004279}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.6; Evidence={ECO:0000256|ARBA:ARBA00024550,
CC ECO:0000256|RuleBase:RU004279};
CC -!- SIMILARITY: Belongs to the RNA polymerase beta' chain family.
CC {ECO:0000256|RuleBase:RU004279}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; KL198007; KDQ29476.1; -; Genomic_DNA.
DR STRING; 1137138.A0A067P0S9; -.
DR VEuPathDB; FungiDB:PLEOSDRAFT_1064266; -.
DR HOGENOM; CLU_000487_2_4_1; -.
DR InParanoid; A0A067P0S9; -.
DR OrthoDB; 169836at2759; -.
DR Proteomes; UP000027073; Unassembled WGS sequence.
DR GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-EC.
DR GO; GO:0006351; P:DNA-templated transcription; IEA:InterPro.
DR CDD; cd02735; RNAP_I_Rpa1_C; 1.
DR CDD; cd01435; RNAP_I_RPA1_N; 1.
DR Gene3D; 1.10.132.30; -; 1.
DR Gene3D; 1.10.150.390; -; 1.
DR Gene3D; 1.10.357.120; -; 1.
DR Gene3D; 2.40.40.20; -; 1.
DR Gene3D; 3.30.70.2850; -; 1.
DR Gene3D; 6.10.250.2940; -; 1.
DR Gene3D; 3.30.1490.180; RNA polymerase ii; 1.
DR Gene3D; 4.10.860.120; RNA polymerase II, clamp domain; 1.
DR Gene3D; 1.10.274.100; RNA polymerase Rpb1, domain 3; 1.
DR InterPro; IPR047107; DNA-dir_RNA_pol1_lsu_C.
DR InterPro; IPR015699; DNA-dir_RNA_pol1_lsu_N.
DR InterPro; IPR045867; DNA-dir_RpoC_beta_prime.
DR InterPro; IPR000722; RNA_pol_asu.
DR InterPro; IPR006592; RNA_pol_N.
DR InterPro; IPR007080; RNA_pol_Rpb1_1.
DR InterPro; IPR007066; RNA_pol_Rpb1_3.
DR InterPro; IPR042102; RNA_pol_Rpb1_3_sf.
DR InterPro; IPR007083; RNA_pol_Rpb1_4.
DR InterPro; IPR007081; RNA_pol_Rpb1_5.
DR InterPro; IPR044893; RNA_pol_Rpb1_clamp_domain.
DR InterPro; IPR038120; Rpb1_funnel_sf.
DR PANTHER; PTHR19376; DNA-DIRECTED RNA POLYMERASE; 1.
DR PANTHER; PTHR19376:SF11; DNA-DIRECTED RNA POLYMERASE I SUBUNIT RPA1; 1.
DR Pfam; PF04997; RNA_pol_Rpb1_1; 1.
DR Pfam; PF00623; RNA_pol_Rpb1_2; 1.
DR Pfam; PF04983; RNA_pol_Rpb1_3; 1.
DR Pfam; PF05000; RNA_pol_Rpb1_4; 1.
DR Pfam; PF04998; RNA_pol_Rpb1_5; 1.
DR SMART; SM00663; RPOLA_N; 1.
DR SUPFAM; SSF64484; beta and beta-prime subunits of DNA dependent RNA-polymerase; 1.
PE 3: Inferred from homology;
KW DNA-directed RNA polymerase {ECO:0000256|ARBA:ARBA00022478,
KW ECO:0000256|RuleBase:RU004279};
KW Nucleotidyltransferase {ECO:0000256|ARBA:ARBA00022695,
KW ECO:0000256|RuleBase:RU004279}; Nucleus {ECO:0000256|ARBA:ARBA00023242};
KW Reference proteome {ECO:0000313|Proteomes:UP000027073};
KW Transcription {ECO:0000256|ARBA:ARBA00023163,
KW ECO:0000256|RuleBase:RU004279};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|RuleBase:RU004279}.
FT DOMAIN 372..711
FT /note="RNA polymerase N-terminal"
FT /evidence="ECO:0000259|SMART:SM00663"
FT REGION 265..322
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1386..1451
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 279..302
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1405..1443
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1713 AA; 191561 MW; 6532A31E63954A9A CRC64;
MNISQSIPST VKSVSFSFLT SDDIRRISVK QIINPVLLDD LNRPNHGGLY DPALGPSERQ
DICATCHLTY FSCPGHFGHI ELPAPVFHPL FMPNMLNLLR GVCLYCHRFK MGRSVMCKYI
AKFRLLERGL LDAAQGIDEI HIRVSGLGKR GVPQDDDEPA EVSEESFEDF QKRINLYVLL
HLQRTENKRD NYKDGQVYQA RKQLIAEFLK TTTLNKCHNE GCFAPAYTFR KEGHTKIIEY
DLTKKQKNSL QGLKRPNVLR EEKLFGDLPA KEVNGSTDQD GDVEMDVDGP ESSPEPSDDE
GEPTDPAALP KSASGKVKTA RGRAERVVIP EECRAHLRRL FRREALICSL LYGRHGHFAP
LTPAGFSLAS PDIFFIDVIP ITPTRFRPPA KMNDTLFEHP QNELLSKVLK TSYRLRDLNN
DLREASEKTA ELDEANRRRI LSMLLDSVLQ LQVDVNSFMD SNKNPSPMPQ GKLPPAGVKQ
LLEKKEGLFR KHMMGKRVNY AARSVISPDV NIEPNEIGIP PVFARKLTYP EPVTPFNFHE
MRQRVITGPH GYPGATMVEY ENGDQLFLDK MSVEQRTAIA NQLLTPQDNE RTKGGLYTRT
PSVNKKVYRH LRDGDMLILN RQPTLHKPSM MAHKARVLQG EKTIRMHYAN CNSYNADFDG
DEMNIHFPQN QVARAEAMFI ANTDNQYLVP TSGNPLRGLI QDHVVAGVWM TCQDAFFTRE
EYFQLLYGSL RPEDDQSRLV TLPPTIWKPK PLWTGKQIIS TILKNITPSH TDGLNLRATA
KVPGPLWGPD SKEDQVVFMN GELLCGVLDK AAFGASDYGL IHSVYELYGA EIAGRLLGIL
SRLFTKFLQH RAFTCRMDDL ILTPDGNAKR VDLLNKDKNL GTEAAIDNFP SLKDTPQNEL
AAALSNLLED VLRDDNKMAG LDMTVKTKLN DLTASITKAC LPSGLYRKFP HNHMQAMTVS
GAKGSAVNAR QISCALGQQE LEGRRVPVMV SGKTLPSFKS FETKAIAGGY VASRFLTGIK
PQEFYFHCMA GREGLIDTAV KTSRSGYLQR CLIKHLEGIR IHYDHTVRGS DQSVYQFHYG
GDAIDVTKQK HLYQFEFVAL NEVSLANLYK PSAVINSIND HEAVAYMKKV LKRPADRPDM
TKPSKRDKYE PALSLYSPSR YLGSTSEQFA TAVARYVKTN PDKLLETKDF AEQMPSRYSP
ISPLHFRLLM NIKYMRSLVE PGEAVGLLAS QSIGEPSTQM TLNTFHFAVG HGAANVTLGI
PRLREIIMTA SRNPKTPSMN MTVRPGVSSE DVKLFCTRAS RLVLSQIIDN VTVKERLKVE
GETRRTEFSV KLSFFPQEEY EAEYDVEPSE IMGCFASKFP LLLKKEMQLE MKRLDADLKS
QIAELGKGKK ERGGAADAAD DEEADEADPK PKRGQDEESE AGDGDADDEK RSRQKKQMAT
YSDDEDEGEG GDEYNEAAIE AAYASDDGEV VEDEAVSSKK RSKLASRVVA DLFMRNLHLA
TTFDFTPSEC NFELQFSPGM PKLLLVGIIE RTCRATVIRE IPGIVDCLQI KESKDDGSVV
KLNTNGSNLK GMWDFATSSD ESILVDNEIY SNDIYAILKT YGVEMARAAI LREMSGVFGA
YSIQVDRRHL ELIADYMTFD GGYKPFNRSG ISTNASPLLK ASYETTAAFL SDATLYGDYD
DLSTPSGNIV LGRPNFTGTG VFDVVMKIDA PSL
//
