ID A0A067P129_PLEOS Unreviewed; 618 AA.
AC A0A067P129;
DT 03-SEP-2014, integrated into UniProtKB/TrEMBL.
DT 03-SEP-2014, sequence version 1.
DT 27-MAR-2024, entry version 29.
DE RecName: Full=Pheromone-processing carboxypeptidase KEX1 {ECO:0000256|ARBA:ARBA00040628};
DE EC=3.4.16.6 {ECO:0000256|ARBA:ARBA00038895};
DE AltName: Full=Carboxypeptidase D {ECO:0000256|ARBA:ARBA00042717};
DE AltName: Full=Pheromone-processing carboxypeptidase kex1 {ECO:0000256|ARBA:ARBA00040403};
GN ORFNames=PLEOSDRAFT_1096672 {ECO:0000313|EMBL:KDQ29586.1};
OS Pleurotus ostreatus PC15.
OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes;
OC Agaricomycetidae; Agaricales; Pleurotineae; Pleurotaceae; Pleurotus.
OX NCBI_TaxID=1137138 {ECO:0000313|EMBL:KDQ29586.1, ECO:0000313|Proteomes:UP000027073};
RN [1] {ECO:0000313|Proteomes:UP000027073}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=PC15 {ECO:0000313|Proteomes:UP000027073};
RX PubMed=24958869; DOI=10.1073/pnas.1400592111;
RA Riley R., Salamov A.A., Brown D.W., Nagy L.G., Floudas D., Held B.W.,
RA Levasseur A., Lombard V., Morin E., Otillar R., Lindquist E.A., Sun H.,
RA LaButti K.M., Schmutz J., Jabbour D., Luo H., Baker S.E., Pisabarro A.G.,
RA Walton J.D., Blanchette R.A., Henrissat B., Martin F., Cullen D.,
RA Hibbett D.S., Grigoriev I.V.;
RT "Extensive sampling of basidiomycete genomes demonstrates inadequacy of the
RT white-rot/brown-rot paradigm for wood decay fungi.";
RL Proc. Natl. Acad. Sci. U.S.A. 111:9923-9928(2014).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Preferential release of a C-terminal arginine or lysine
CC residue.; EC=3.4.16.6; Evidence={ECO:0000256|ARBA:ARBA00001003};
CC -!- SUBCELLULAR LOCATION: Golgi apparatus, trans-Golgi network membrane
CC {ECO:0000256|ARBA:ARBA00004393}; Single-pass type I membrane protein
CC {ECO:0000256|ARBA:ARBA00004393}. Membrane
CC {ECO:0000256|ARBA:ARBA00004479}; Single-pass type I membrane protein
CC {ECO:0000256|ARBA:ARBA00004479}.
CC -!- SIMILARITY: Belongs to the peptidase S10 family.
CC {ECO:0000256|ARBA:ARBA00009431}.
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DR EMBL; KL198007; KDQ29586.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A067P129; -.
DR STRING; 1137138.A0A067P129; -.
DR MEROPS; S10.007; -.
DR VEuPathDB; FungiDB:PLEOSDRAFT_1096672; -.
DR HOGENOM; CLU_008523_11_1_1; -.
DR InParanoid; A0A067P129; -.
DR OrthoDB; 1647009at2759; -.
DR Proteomes; UP000027073; Unassembled WGS sequence.
DR GO; GO:0005794; C:Golgi apparatus; IEA:UniProtKB-SubCell.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0004185; F:serine-type carboxypeptidase activity; IEA:InterPro.
DR GO; GO:0006915; P:apoptotic process; IEA:UniProtKB-KW.
DR GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR Gene3D; 3.40.50.1820; alpha/beta hydrolase; 1.
DR InterPro; IPR029058; AB_hydrolase.
DR InterPro; IPR001563; Peptidase_S10.
DR InterPro; IPR033124; Ser_caboxypep_his_AS.
DR PANTHER; PTHR11802:SF190; PHEROMONE-PROCESSING CARBOXYPEPTIDASE KEX1; 1.
DR PANTHER; PTHR11802; SERINE PROTEASE FAMILY S10 SERINE CARBOXYPEPTIDASE; 1.
DR Pfam; PF00450; Peptidase_S10; 1.
DR PRINTS; PR00724; CRBOXYPTASEC.
DR SUPFAM; SSF53474; alpha/beta-Hydrolases; 1.
DR PROSITE; PS00560; CARBOXYPEPT_SER_HIS; 1.
PE 3: Inferred from homology;
KW Apoptosis {ECO:0000256|ARBA:ARBA00022703};
KW Carboxypeptidase {ECO:0000256|ARBA:ARBA00022645};
KW Glycoprotein {ECO:0000256|ARBA:ARBA00023180};
KW Golgi apparatus {ECO:0000256|ARBA:ARBA00023034};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW Membrane {ECO:0000256|ARBA:ARBA00023136, ECO:0000256|SAM:Phobius};
KW Protease {ECO:0000256|ARBA:ARBA00022670};
KW Reference proteome {ECO:0000313|Proteomes:UP000027073};
KW Signal {ECO:0000256|ARBA:ARBA00022729, ECO:0000256|SAM:SignalP};
KW Transmembrane {ECO:0000256|ARBA:ARBA00022692, ECO:0000256|SAM:Phobius};
KW Transmembrane helix {ECO:0000256|ARBA:ARBA00022989,
KW ECO:0000256|SAM:Phobius}.
FT SIGNAL 1..25
FT /evidence="ECO:0000256|SAM:SignalP"
FT CHAIN 26..618
FT /note="Pheromone-processing carboxypeptidase KEX1"
FT /evidence="ECO:0000256|SAM:SignalP"
FT /id="PRO_5001642772"
FT TRANSMEM 517..541
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT REGION 552..618
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 556..570
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 571..595
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 604..618
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 618 AA; 68323 MW; 9FB6989198BB2184 CRC64;
MRSTLCTKII LFGWAVAASI RGTLAASAGS DVPSAASFYV PSLPDLHQDS LHPLRIYSGH
LSSDTSKDAS SSTDVTPHLF FVLVKNRRTA DKERVMFWFN GGPGCSSFDG LMMEVGPWRM
DGKGGFKVVE GGWEEYTTMV YIDQPAGTGF SYTSTNKLVE GMSEASAQLL VFLENFYKVF
PEYRTMDTYL GGESFAGQYI PFFADAILNS SLGTRLMGVA IGNGWIDGRR QYPAYIDYAV
KMGILVENSE DWKYAKAETD ACVAEFDKFT EFEPGNVPRC ERLVLDVARG RNKEKDGSPM
CINIYDVRYD DTKPACGMNW PPDIKPITTY LDRPEVVKAL HAEAHSEAWV ECRGAIHSAF
NRRENNSSIT ILPSVIERIP VLIFAGDQDL ICNHVGLEAM IQAMTWNGAK GLGVVQTQSW
SVAGTPAGTW VSSRNLTYAK IFNASHMAPF DVPHVTHDMI LRFMGVNFSA IMDGSARIPS
SVGTIMKPIF IEDSEASKTP APVPGKTPEQ DKAMWEAYYN AGSAALVLVL IFLALGTFLW
CRIRRRRVQL PVSHQDEESI PLNSSIAMNG RQDGEEEDSA SFKSRKGKER AVEPPEPPIF
ELGESDDEDM KSPSRKQS
//
