ID A0A072NWE1_9EURO Unreviewed; 1078 AA.
AC A0A072NWE1;
DT 01-OCT-2014, integrated into UniProtKB/TrEMBL.
DT 01-OCT-2014, sequence version 1.
DT 27-MAR-2024, entry version 36.
DE SubName: Full=Regulator-nonsense transcripts 1 {ECO:0000313|EMBL:KEF51328.1};
GN ORFNames=A1O9_12678 {ECO:0000313|EMBL:KEF51328.1};
OS Exophiala aquamarina CBS 119918.
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC Chaetothyriomycetidae; Chaetothyriales; Herpotrichiellaceae; Exophiala.
OX NCBI_TaxID=1182545 {ECO:0000313|EMBL:KEF51328.1, ECO:0000313|Proteomes:UP000027920};
RN [1] {ECO:0000313|EMBL:KEF51328.1, ECO:0000313|Proteomes:UP000027920}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CBS 119918 {ECO:0000313|EMBL:KEF51328.1,
RC ECO:0000313|Proteomes:UP000027920};
RG The Broad Institute Genomics Platform;
RA Cuomo C., de Hoog S., Gorbushina A., Walker B., Young S.K., Zeng Q.,
RA Gargeya S., Fitzgerald M., Haas B., Abouelleil A., Allen A.W., Alvarado L.,
RA Arachchi H.M., Berlin A.M., Chapman S.B., Gainer-Dewar J., Goldberg J.,
RA Griggs A., Gujja S., Hansen M., Howarth C., Imamovic A., Ireland A.,
RA Larimer J., McCowan C., Murphy C., Pearson M., Poon T.W., Priest M.,
RA Roberts A., Saif S., Shea T., Sisk P., Sykes S., Wortman J., Nusbaum C.,
RA Birren B.;
RT "The Genome Sequence of Exophiala aquamarina CBS 119918.";
RL Submitted (MAR-2013) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.12;
CC Evidence={ECO:0000256|ARBA:ARBA00000600};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:13066;
CC Evidence={ECO:0000256|ARBA:ARBA00000600};
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|ARBA:ARBA00004496}.
CC -!- SIMILARITY: Belongs to the DNA2/NAM7 helicase family.
CC {ECO:0000256|ARBA:ARBA00007913}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KEF51328.1}.
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DR EMBL; AMGV01000025; KEF51328.1; -; Genomic_DNA.
DR RefSeq; XP_013253918.1; XM_013398464.1.
DR AlphaFoldDB; A0A072NWE1; -.
DR STRING; 1182545.A0A072NWE1; -.
DR GeneID; 25287572; -.
DR VEuPathDB; FungiDB:A1O9_12678; -.
DR HOGENOM; CLU_001666_4_0_1; -.
DR OrthoDB; 170190at2759; -.
DR Proteomes; UP000027920; Unassembled WGS sequence.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:InterPro.
DR GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR GO; GO:0003724; F:RNA helicase activity; IEA:InterPro.
DR GO; GO:0008270; F:zinc ion binding; IEA:UniProtKB-UniRule.
DR GO; GO:0000184; P:nuclear-transcribed mRNA catabolic process, nonsense-mediated decay; IEA:InterPro.
DR CDD; cd21407; 1B_UPF1-like; 1.
DR CDD; cd18039; DEXXQc_UPF1; 1.
DR CDD; cd18808; SF1_C_Upf1; 1.
DR CDD; cd21400; ZBD_UPF1-like; 1.
DR Gene3D; 2.40.30.230; -; 1.
DR Gene3D; 6.10.140.1240; -; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR InterPro; IPR045055; DNA2/NAM7-like.
DR InterPro; IPR041679; DNA2/NAM7-like_C.
DR InterPro; IPR041677; DNA2/NAM7_AAA_11.
DR InterPro; IPR014001; Helicase_ATP-bd.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR047187; SF1_C_Upf1.
DR InterPro; IPR040812; UPF1_1B_dom.
DR InterPro; IPR018999; UPF1_CH/ZBD.
DR PANTHER; PTHR10887; DNA2/NAM7 HELICASE FAMILY; 1.
DR PANTHER; PTHR10887:SF364; REGULATOR OF NONSENSE TRANSCRIPTS 1; 1.
DR Pfam; PF13086; AAA_11; 1.
DR Pfam; PF13087; AAA_12; 1.
DR Pfam; PF18141; UPF1_1B_dom; 1.
DR Pfam; PF09416; UPF1_Zn_bind; 1.
DR SMART; SM00487; DEXDc; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR PROSITE; PS51997; UPF1_CH_RICH; 1.
PE 3: Inferred from homology;
KW Cytoplasm {ECO:0000256|ARBA:ARBA00022490};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723, ECO:0000256|PROSITE-
KW ProRule:PRU01341}; Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW Reference proteome {ECO:0000313|Proteomes:UP000027920};
KW Zinc {ECO:0000256|PROSITE-ProRule:PRU01341};
KW Zinc-finger {ECO:0000256|PROSITE-ProRule:PRU01341}.
FT DOMAIN 84..241
FT /note="Upf1"
FT /evidence="ECO:0000259|PROSITE:PS51997"
FT REGION 23..84
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 92..124
FT /note="C3H"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01341"
FT REGION 106..134
FT /note="CC/SHH/C"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01341"
FT REGION 152..182
FT /note="C4"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01341"
SQ SEQUENCE 1078 AA; 118871 MW; 3C9B1F7AA740D9CF CRC64;
MEAFSNMGNH LVSNSAAAIN ATDDVSTLDP DESVLSLAQG PRRRQHDDDD DESESVEEDD
LESLASAAVD GKKQPTKPQD LEPEKELPPH ACAYCGIHNP STVVRCLSCS KWFCSARGNT
SSSHIVNHLV RARHKEVQLH PSSSLGDTVL ECYNCGTKNV FLLGFIPAKS DTVVVLLCRQ
PCAAMPSSKD MNWDTSRWQP LIEDRSFLSW LVAQPTDQEQ LRARHLSPQM IAKLEELWKE
NAQATITDLE KSANIDDEPA PVLLRYDDAY QYQNVFGPLV KIEADYDRKL KESQSQDNLT
VRWDVGLNNK HLASFVLPKL ELGDVKLAVG DEMRLKYAGE LRPVWEGVGY VIKIPNNQSD
EVTIELRSKG DHKSVPTECT HNFTADYVWK ATSFDRMQLA MKTFAIDEMS VSGYIFHRLL
GHEVAAAPMK TQLPKKFSVP GLPELNGSQI NAVKSVLQKP LSLIQGPPGT GKTVTSATII
YHLSKTNGGQ VLVCAPSNVA VDQLCERVHR TGLKTVRVTA KSREDVESPV GFLSLHEQVR
INDSNVELTK LNQLKSELGE LSSQDEKKYK SLTRAAEREI LTNADVICCT CVGAGDPRLA
KFKFRTVLID ESTQSAEPEC MIPLVLGCKQ VVLVGDHQQL GPVIMNKKAA KAGLNQSLFE
RLVILGCAPI RLNVQYRMHP CLSEFPSNMF YEGSLQNGVT MQDRLRKDVD FPWPVAESPM
MFWSNLGNEE ISASGTSYLN RTEAANVEKI VTRFFKAGVK PSGIGVITPY EGQRSYIVSS
MQANGTFKKE LYKEIEVASV DAFQGREKDF IILSCVRSND HQGIGFLSDP RRLNVAMTRA
KFGLVILGNP KVLSKHPLWH YLLLHYKERN CLVEGPLTNL QISLHQFSRP KQSYRGPQRY
QMAYNHASHM ANGMMNGRAP QRGEYREGGS VVGYIPDDVS SIHSSAIGGV GVPAGYPPMF
QGFTPDNWPS VSGVNGRRPN GVKPRPFTAS VAGESTVATE SDITGSGIGG VTLDQLSIHD
TNKPASYNQA DRLKRYVEGG GPNIGYLGNH KVGKGIHEDE DARSVSTAFA SQIGGGYD
//
