ID A0A072TDN8_MEDTR Unreviewed; 1936 AA.
AC A0A072TDN8;
DT 01-OCT-2014, integrated into UniProtKB/TrEMBL.
DT 01-OCT-2014, sequence version 1.
DT 27-MAR-2024, entry version 41.
DE SubName: Full=Conjugal transfer protein TrbG {ECO:0000313|EMBL:KEH15674.1};
DE Flags: Fragment;
GN ORFNames=MTR_0666s0020 {ECO:0000313|EMBL:KEH15674.1};
OS Medicago truncatula (Barrel medic) (Medicago tribuloides).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade;
OC NPAAA clade; Hologalegina; IRL clade; Trifolieae; Medicago.
OX NCBI_TaxID=3880 {ECO:0000313|EMBL:KEH15674.1, ECO:0000313|Proteomes:UP000002051};
RN [1] {ECO:0000313|EMBL:KEH15674.1, ECO:0000313|Proteomes:UP000002051}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=A17 {ECO:0000313|EMBL:KEH15674.1}, and cv. Jemalong A17
RC {ECO:0000313|EnsemblPlants:KEH15674,
RC ECO:0000313|Proteomes:UP000002051};
RX PubMed=22089132; DOI=10.1038/nature10625;
RA Young N.D., Debelle F., Oldroyd G.E., Geurts R., Cannon S.B., Udvardi M.K.,
RA Benedito V.A., Mayer K.F., Gouzy J., Schoof H., Van de Peer Y., Proost S.,
RA Cook D.R., Meyers B.C., Spannagl M., Cheung F., De Mita S.,
RA Krishnakumar V., Gundlach H., Zhou S., Mudge J., Bharti A.K., Murray J.D.,
RA Naoumkina M.A., Rosen B., Silverstein K.A., Tang H., Rombauts S.,
RA Zhao P.X., Zhou P., Barbe V., Bardou P., Bechner M., Bellec A., Berger A.,
RA Berges H., Bidwell S., Bisseling T., Choisne N., Couloux A., Denny R.,
RA Deshpande S., Dai X., Doyle J.J., Dudez A.M., Farmer A.D., Fouteau S.,
RA Franken C., Gibelin C., Gish J., Goldstein S., Gonzalez A.J., Green P.J.,
RA Hallab A., Hartog M., Hua A., Humphray S.J., Jeong D.H., Jing Y.,
RA Jocker A., Kenton S.M., Kim D.J., Klee K., Lai H., Lang C., Lin S.,
RA Macmil S.L., Magdelenat G., Matthews L., McCorrison J., Monaghan E.L.,
RA Mun J.H., Najar F.Z., Nicholson C., Noirot C., O'Bleness M., Paule C.R.,
RA Poulain J., Prion F., Qin B., Qu C., Retzel E.F., Riddle C., Sallet E.,
RA Samain S., Samson N., Sanders I., Saurat O., Scarpelli C., Schiex T.,
RA Segurens B., Severin A.J., Sherrier D.J., Shi R., Sims S., Singer S.R.,
RA Sinharoy S., Sterck L., Viollet A., Wang B.B., Wang K., Wang M., Wang X.,
RA Warfsmann J., Weissenbach J., White D.D., White J.D., Wiley G.B.,
RA Wincker P., Xing Y., Yang L., Yao Z., Ying F., Zhai J., Zhou L., Zuber A.,
RA Denarie J., Dixon R.A., May G.D., Schwartz D.C., Rogers J., Quetier F.,
RA Town C.D., Roe B.A.;
RT "The Medicago genome provides insight into the evolution of rhizobial
RT symbioses.";
RL Nature 480:520-524(2011).
RN [2] {ECO:0000313|EMBL:KEH15674.1, ECO:0000313|Proteomes:UP000002051}
RP GENOME REANNOTATION.
RC STRAIN=A17 {ECO:0000313|EMBL:KEH15674.1}, and cv. Jemalong A17
RC {ECO:0000313|EnsemblPlants:KEH15674,
RC ECO:0000313|Proteomes:UP000002051};
RX PubMed=24767513; DOI=10.1186/1471-2164-15-312;
RA Tang H., Krishnakumar V., Bidwell S., Rosen B., Chan A., Zhou S.,
RA Gentzbittel L., Childs K.L., Yandell M., Gundlach H., Mayer K.F.,
RA Schwartz D.C., Town C.D.;
RT "An improved genome release (version Mt4.0) for the model legume Medicago
RT truncatula.";
RL BMC Genomics 15:312-312(2014).
RN [3] {ECO:0000313|EnsemblPlants:KEH15674}
RP IDENTIFICATION.
RC STRAIN=cv. Jemalong A17 {ECO:0000313|EnsemblPlants:KEH15674};
RG EnsemblPlants;
RL Submitted (JUN-2015) to UniProtKB.
CC -!- SIMILARITY: Belongs to the TrbG/VirB9 family.
CC {ECO:0000256|ARBA:ARBA00006135}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; KL403390; KEH15674.1; -; Genomic_DNA.
DR RefSeq; XP_013441649.1; XM_013586195.1.
DR EnsemblPlants; KEH15674; KEH15674; MTR_0666s0020.
DR Gramene; KEH15674; KEH15674; MTR_0666s0020.
DR HOGENOM; CLU_235104_0_0_1; -.
DR Proteomes; UP000002051; Unassembled WGS sequence.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR GO; GO:0009306; P:protein secretion; IEA:InterPro.
DR CDD; cd16425; TrbF; 1.
DR CDD; cd16429; VirB10; 1.
DR CDD; cd06911; VirB9_CagX_TrbG; 1.
DR Gene3D; 2.60.40.2500; -; 1.
DR Gene3D; 2.40.128.260; Type IV secretion system, VirB10/TraB/TrbI; 1.
DR Gene3D; 3.10.450.230; VirB8 protein; 1.
DR InterPro; IPR010258; Conjugal_tfr_TrbG/VirB9/CagX.
DR InterPro; IPR032710; NTF2-like_dom_sf.
DR InterPro; IPR018927; Pilus_synth_Q_C.
DR InterPro; IPR004846; T2SS/T3SS_dom.
DR InterPro; IPR005498; T4SS_VirB10/TraB/TrbI.
DR InterPro; IPR042217; T4SS_VirB10/TrbI.
DR InterPro; IPR035658; TrbF.
DR InterPro; IPR007430; VirB8.
DR InterPro; IPR033645; VirB9/CagX/TrbG_C.
DR InterPro; IPR038161; VirB9/CagX/TrbG_C_sf.
DR PANTHER; PTHR30332; PROBABLE GENERAL SECRETION PATHWAY PROTEIN D; 1.
DR PANTHER; PTHR30332:SF28; TYPE IV PILIATION SYSTEM PROTEIN DR_0774-RELATED; 1.
DR Pfam; PF03524; CagX; 1.
DR Pfam; PF00263; Secretin; 1.
DR Pfam; PF10671; TcpQ; 1.
DR Pfam; PF03743; TrbI; 1.
DR Pfam; PF04335; VirB8; 1.
DR SUPFAM; SSF54427; NTF2-like; 1.
PE 3: Inferred from homology;
KW Membrane {ECO:0000256|ARBA:ARBA00023136, ECO:0000256|SAM:Phobius};
KW Reference proteome {ECO:0000313|Proteomes:UP000002051};
KW Signal {ECO:0000256|ARBA:ARBA00022729};
KW Transmembrane {ECO:0000256|ARBA:ARBA00022692, ECO:0000256|SAM:Phobius};
KW Transmembrane helix {ECO:0000256|ARBA:ARBA00022989,
KW ECO:0000256|SAM:Phobius}.
FT TRANSMEM 53..77
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT DOMAIN 36..232
FT /note="Bacterial virulence protein VirB8"
FT /evidence="ECO:0000259|Pfam:PF04335"
FT DOMAIN 1349..1423
FT /note="Toxin co-regulated pilus biosynthesis protein Q C-
FT terminal"
FT /evidence="ECO:0000259|Pfam:PF10671"
FT DOMAIN 1801..1934
FT /note="Type II/III secretion system secretin-like"
FT /evidence="ECO:0000259|Pfam:PF00263"
FT REGION 1..31
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 467..503
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 633..661
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1047..1079
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1297..1345
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1629..1664
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..16
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 467..484
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 637..661
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1629..1663
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT NON_TER 1936
FT /evidence="ECO:0000313|EMBL:KEH15674.1"
SQ SEQUENCE 1936 AA; 202900 MW; FA6C6B1A74FAE8D5 CRC64;
MCAMKTSAQK SAPRSDSSKG PPVPPYTPGP IRESATRKYF ARGEMERITA NRWFLATVFL
AVLTLVNAIG WVVFLPLKTI QTIVINQEKS GRLIADLNGT SSYVPDRDAV AYFLSEWINN
VYDINASTID NNLALAGKMT VGEATDQLNS LLHKENPYAR LHDVPTLRRT FNRLTANFIT
DDTVIIRFNL TEREGPGSSP KVSAWAMTIT YTRIPPTTIE QVKINPGFVA VSTAEAGGNR
NAADTAISSP LDNRIVTFPY TADAVFTLLT QSGQGTHIQL ERGEGVVEKP VLGDTIQWRI
SGGPTNLYVK PVRPGISTSL TLVTNKRTYE FYLVASEPGG KFYQQVSFYY PDELKDVQLQ
AQVQAESFIA EKQRLSSQLL TPPLDPTSFH YGYKIKGDAP FRPIDVFDDG TKTFFRIPNV
QDMPAVFVPD DSKRLTLIVT RLQGNFVIAD RVSDRFVLKL DDQEDDESAF EHGNPARRRV
DRNDIPGSVQ APDREPVEDL TRGKRKRGKT LGFLVILLAL GGGLYYLYGQ PDKPSQRRKT
NATYTPSEQA GALVVGQAKD AAAQTAEDRR RAALMQGASG VKAVDPNAAA GAAIGGQALV
GNGAAATPAS DAAAAAKAAK DAAEARAERE AQIAASPLQA NDVQTLKEES GRNSQPQSPA
EELAATLKNQ LKENQSVAEK QMDKVMQLSA AYGGAGQGGA AVPMATRSGQ QDQWLASQKA
DGQGPMQMNA APAMPIVSEG TPVRAVLLTG LDTDNPGQVS AMVTSDVYDT FTGRALMIPK
GSRLNGSYNH EIHVGQDRVL IAMTRLVRPD GSWIDLSGAS GGEMDGSSGL EGNVNNHFWK
IFGSSLVIGA ATLLLDRTQQ NVTVSQGLGT TQMGGTIFAQ TLQQVLTDLL SRNKDIPPTI
TRSGGTQFTF LVRRDLALSP YFRSLVMGPL LASAAPGLDF GYRIDGPASV RPTLVFNDGL
DTYVQPSGSG RTYVAGAKAD GPYLRITGIP DSFAVRVGSI SLRVDHVGLP SGPSSLASSP
STSHSYGERV AGLRSALAVR AAASPAGAPL SRTSSSAVSR SGVDGAAAGD GSGSEQTAVG
GPIATDAVAA TSIPGVAPGN ESPNVGKKSE STDVSYLAKY FGADGIREGF GGAIQIHFRT
RPSAELQFAS ADGKHLDASW DDTANVMTVG AAQKFVVRDG RSSVAVSRQT TDTFNYPADN
AAGLEQVFSE SGAIYFRVAE GTKKVSVRAD GKALRGQQKG RYYRVAGTAD SFVVNADGFE
ESSMRIRVAV LALGALFLAG LSGSAVAAGG PPTPVGAGWQ PLRGGGQPAA ERGSYAGAPA
AANPESASQA VAEPGPAKGP EEATPAAEVW TIREGFPISE ELKSWGSRAN WSVVWQMPRD
VIAAATTTFS GDFPTALTDV VKTLAANGAL IRARIFDGNR TVVGLRCAAL TAVVAALLAG
CAQIAAGDRT VKAAADKVIE EQAKASIEGP VVSRTTGAWL LGDSVQVTPE LSPILKRQIA
WQPPQRVTLS ELAAYVTKQT GLSVDISEVQ RPAGAGKTGM ASTSAAGQLP LPPGMLAAPA
IAKPGGDAAA SEALPTMQVS YVGNVKGLLD FAANEVGLWW RMENGGVEFY RTVTKTIYIA
AVNRKSSGQN SIVTQSGPGS LSNSSSSGSG GGSSGSPTGN SMSKDEYDVD VWKTIASTAR
TVAGASGGGL AEISAEPAQG SITVTGSPTQ VKNVEAWAKG LTDKLSQQVL ISVSVYSVNL
SNEDNYGWSP TVLYSKLSTM YGLSLTPPGV PQVTGSLAPM LLTGTAGKSS SLNGSQLAVN
ALSTLGHVSE TVTQSVVTLN GQPAPMQVAN QVTYLAQSGT TNTANVGSST TLTPGVVTTG
FTATFLPQIV NGRILLTMDM TNSVLNSINQ ASSNGSTIQT PNVSSTTFQQ SVSLTPGAAL
MLAGLQQDNS AVNRSG
//
