ID A0A075JG44_9MICO Unreviewed; 427 AA.
AC A0A075JG44;
DT 29-OCT-2014, integrated into UniProtKB/TrEMBL.
DT 29-OCT-2014, sequence version 1.
DT 24-JAN-2024, entry version 44.
DE RecName: Full=isocitrate lyase {ECO:0000256|ARBA:ARBA00012909};
DE EC=4.1.3.1 {ECO:0000256|ARBA:ARBA00012909};
DE AltName: Full=Isocitrase {ECO:0000256|ARBA:ARBA00031022};
DE AltName: Full=Isocitratase {ECO:0000256|ARBA:ARBA00031921};
GN Name=aceA {ECO:0000313|EMBL:TJZ98612.1};
GN ORFNames=FA951_01025 {ECO:0000313|EMBL:TJZ98612.1}, HX89_09675
GN {ECO:0000313|EMBL:AIF41171.1};
OS Dermacoccus nishinomiyaensis.
OC Bacteria; Actinomycetota; Actinomycetes; Micrococcales; Dermacoccaceae;
OC Dermacoccus.
OX NCBI_TaxID=1274 {ECO:0000313|EMBL:AIF41171.1, ECO:0000313|Proteomes:UP000027986};
RN [1] {ECO:0000313|EMBL:AIF41171.1, ECO:0000313|Proteomes:UP000027986}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=M25 {ECO:0000313|EMBL:AIF41171.1,
RC ECO:0000313|Proteomes:UP000027986};
RA Hong K.W., Chan K.G.;
RT "Genome Sequencing of Dermacoccus nishinomiyaensis.";
RL Submitted (JUL-2014) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|EMBL:TJZ98612.1, ECO:0000313|Proteomes:UP000307824}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=TSA37 {ECO:0000313|EMBL:TJZ98612.1,
RC ECO:0000313|Proteomes:UP000307824};
RA Williams A.N., Maclea K.S.;
RT "Genome sequence of Dermacoccus nishinomiyaensis strain TSA37.";
RL Submitted (APR-2019) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=D-threo-isocitrate = glyoxylate + succinate;
CC Xref=Rhea:RHEA:13245, ChEBI:CHEBI:15562, ChEBI:CHEBI:30031,
CC ChEBI:CHEBI:36655; EC=4.1.3.1;
CC Evidence={ECO:0000256|ARBA:ARBA00023531};
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000256|PIRSR:PIRSR001362-3};
CC Note=Can also use Mn(2+) ion. {ECO:0000256|PIRSR:PIRSR001362-3};
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DR EMBL; CP008889; AIF41171.1; -; Genomic_DNA.
DR EMBL; SUPQ01000001; TJZ98612.1; -; Genomic_DNA.
DR RefSeq; WP_006944896.1; NZ_VECS01000001.1.
DR AlphaFoldDB; A0A075JG44; -.
DR STRING; 1274.HX89_09675; -.
DR GeneID; 41841407; -.
DR KEGG; dni:HX89_09675; -.
DR eggNOG; COG2224; Bacteria.
DR HOGENOM; CLU_019214_2_0_11; -.
DR OrthoDB; 8629576at2; -.
DR Proteomes; UP000027986; Chromosome.
DR Proteomes; UP000307824; Unassembled WGS sequence.
DR GO; GO:0004451; F:isocitrate lyase activity; IEA:UniProtKB-EC.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0019752; P:carboxylic acid metabolic process; IEA:InterPro.
DR CDD; cd00377; ICL_PEPM; 1.
DR Gene3D; 3.20.20.60; Phosphoenolpyruvate-binding domains; 1.
DR InterPro; IPR039556; ICL/PEPM.
DR InterPro; IPR006254; Isocitrate_lyase.
DR InterPro; IPR018523; Isocitrate_lyase_ph_CS.
DR InterPro; IPR015813; Pyrv/PenolPyrv_Kinase-like_dom.
DR InterPro; IPR040442; Pyrv_Kinase-like_dom_sf.
DR NCBIfam; TIGR01346; isocit_lyase; 2.
DR PANTHER; PTHR21631:SF3; BIFUNCTIONAL GLYOXYLATE CYCLE PROTEIN; 1.
DR PANTHER; PTHR21631; ISOCITRATE LYASE/MALATE SYNTHASE; 1.
DR Pfam; PF00463; ICL; 2.
DR PIRSF; PIRSF001362; Isocit_lyase; 1.
DR SUPFAM; SSF51621; Phosphoenolpyruvate/pyruvate domain; 1.
DR PROSITE; PS00161; ISOCITRATE_LYASE; 1.
PE 4: Predicted;
KW Lyase {ECO:0000256|ARBA:ARBA00023239, ECO:0000313|EMBL:AIF41171.1};
KW Magnesium {ECO:0000256|PIRSR:PIRSR001362-3};
KW Metal-binding {ECO:0000256|PIRSR:PIRSR001362-3};
KW Reference proteome {ECO:0000313|Proteomes:UP000027986}.
FT ACT_SITE 191
FT /note="Proton acceptor"
FT /evidence="ECO:0000256|PIRSR:PIRSR001362-1"
FT BINDING 91..93
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR001362-2"
FT BINDING 153
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000256|PIRSR:PIRSR001362-3"
FT BINDING 192..193
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR001362-2"
FT BINDING 228
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR001362-2"
FT BINDING 313..317
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR001362-2"
FT BINDING 347
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR001362-2"
SQ SEQUENCE 427 AA; 47470 MW; 161D2E66BBA74C0F CRC64;
MTGENIQARA AEIQKDWDTN ERWKNVKRDY SAEDVVRLQG SIQEEFTLAK HGAENLWEKL
HTEDFVNALG ALTGNQAVQQ VKAGLKAIYL SGWQVAGDAN LAAQTYPDQS LYPANSVPQV
VRRINNALMR ADQIEFSEGI KSVDEWLVPI VADAEAGFGG PLNAYELMKS MIAAGAAGVH
WEDQLASEKK CGHLGGKVLI PTKQHVRTLN AARLATDVSN TPTVVIARTD AEAATLITSD
VDERDQQFLT GERTNEGFYK VKNGIEPCIE RAKAYAPYAD LIWMETGTPD LELAKKFAES
VKAEFPDQML AYNCSPSFNW KKHLDDDTIA KFQRELGAMG FKFQFITLAG FHALNYSMFD
LAHGYAREQM KAYVELQERE FASESRGYTA TRHQREVGTG YFDLVATTLN PESSTTALKE
STESAQF
//
