ID A0A078F3G6_BRANA Unreviewed; 912 AA.
AC A0A078F3G6;
DT 29-OCT-2014, integrated into UniProtKB/TrEMBL.
DT 29-OCT-2014, sequence version 1.
DT 27-MAR-2024, entry version 37.
DE RecName: Full=Non-lysosomal glucosylceramidase {ECO:0000256|PIRNR:PIRNR028944};
DE Short=NLGase {ECO:0000256|PIRNR:PIRNR028944};
DE EC=3.2.1.45 {ECO:0000256|PIRNR:PIRNR028944};
GN Name=BnaC01g30560D {ECO:0000313|EMBL:CDY09035.1};
GN ORFNames=GSBRNA2T00001176001 {ECO:0000313|EMBL:CDY09035.1};
OS Brassica napus (Rape).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Brassiceae; Brassica.
OX NCBI_TaxID=3708 {ECO:0000313|EMBL:CDY09035.1, ECO:0000313|Proteomes:UP000028999};
RN [1] {ECO:0000313|EMBL:CDY09035.1, ECO:0000313|Proteomes:UP000028999}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Darmor-bzh {ECO:0000313|Proteomes:UP000028999};
RX PubMed=25146293; DOI=10.1126/science.1253435;
RA Chalhoub B., Denoeud F., Liu S., Parkin I.A., Tang H., Wang X., Chiquet J.,
RA Belcram H., Tong C., Samans B., Correa M., Da Silva C., Just J.,
RA Falentin C., Koh C.S., Le Clainche I., Bernard M., Bento P., Noel B.,
RA Labadie K., Alberti A., Charles M., Arnaud D., Guo H., Daviaud C.,
RA Alamery S., Jabbari K., Zhao M., Edger P.P., Chelaifa H., Tack D.,
RA Lassalle G., Mestiri I., Schnel N., Le Paslier M.C., Fan G., Renault V.,
RA Bayer P.E., Golicz A.A., Manoli S., Lee T.H., Thi V.H., Chalabi S., Hu Q.,
RA Fan C., Tollenaere R., Lu Y., Battail C., Shen J., Sidebottom C.H.,
RA Wang X., Canaguier A., Chauveau A., Berard A., Deniot G., Guan M., Liu Z.,
RA Sun F., Lim Y.P., Lyons E., Town C.D., Bancroft I., Wang X., Meng J.,
RA Ma J., Pires J.C., King G.J., Brunel D., Delourme R., Renard M., Aury J.M.,
RA Adams K.L., Batley J., Snowdon R.J., Tost J., Edwards D., Zhou Y., Hua W.,
RA Sharpe A.G., Paterson A.H., Guan C., Wincker P.;
RT "Plant genetics. Early allopolyploid evolution in the post-Neolithic
RT Brassica napus oilseed genome.";
RL Science 345:950-953(2014).
CC -!- FUNCTION: Non-lysosomal glucosylceramidase that catalyzes the
CC hydrolysis of glucosylceramide (GlcCer) to free glucose and ceramide.
CC {ECO:0000256|PIRNR:PIRNR028944}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a beta-D-glucosyl-(1<->1')-N-acylsphing-4-enine + H2O = an N-
CC acylsphing-4-enine + D-glucose; Xref=Rhea:RHEA:13269,
CC ChEBI:CHEBI:4167, ChEBI:CHEBI:15377, ChEBI:CHEBI:22801,
CC ChEBI:CHEBI:52639; EC=3.2.1.45;
CC Evidence={ECO:0000256|PIRNR:PIRNR028944};
CC -!- SIMILARITY: Belongs to the non-lysosomal glucosylceramidase family.
CC {ECO:0000256|PIRNR:PIRNR028944}.
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DR EMBL; LK031991; CDY09035.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A078F3G6; -.
DR STRING; 3708.A0A078F3G6; -.
DR PaxDb; 3708-A0A078F3G6; -.
DR EnsemblPlants; CDY09035; CDY09035; GSBRNA2T00001176001.
DR Gramene; CDY09035; CDY09035; GSBRNA2T00001176001.
DR OMA; PTTHETF; -.
DR Proteomes; UP000028999; Unassembled WGS sequence.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR GO; GO:0008422; F:beta-glucosidase activity; IBA:GO_Central.
DR GO; GO:0004348; F:glucosylceramidase activity; IEA:UniProtKB-EC.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR GO; GO:0006680; P:glucosylceramide catabolic process; IEA:InterPro.
DR Gene3D; 1.50.10.10; -; 1.
DR InterPro; IPR008928; 6-hairpin_glycosidase_sf.
DR InterPro; IPR012341; 6hp_glycosidase-like_sf.
DR InterPro; IPR014551; B_Glucosidase_GBA2-typ.
DR InterPro; IPR006775; GH116_catalytic.
DR InterPro; IPR024462; GH116_N.
DR PANTHER; PTHR12654; BILE ACID BETA-GLUCOSIDASE-RELATED; 1.
DR PANTHER; PTHR12654:SF0; NON-LYSOSOMAL GLUCOSYLCERAMIDASE; 1.
DR Pfam; PF04685; DUF608; 1.
DR Pfam; PF12215; Glyco_hydr_116N; 1.
DR PIRSF; PIRSF028944; Beta_gluc_GBA2; 1.
DR SUPFAM; SSF48208; Six-hairpin glycosidases; 1.
PE 3: Inferred from homology;
KW Glycosidase {ECO:0000256|PIRNR:PIRNR028944};
KW Hydrolase {ECO:0000256|PIRNR:PIRNR028944};
KW Lipid metabolism {ECO:0000256|PIRNR:PIRNR028944};
KW Membrane {ECO:0000256|PIRNR:PIRNR028944};
KW Reference proteome {ECO:0000313|Proteomes:UP000028999}.
FT DOMAIN 102..421
FT /note="Glycosyl-hydrolase family 116 N-terminal"
FT /evidence="ECO:0000259|Pfam:PF12215"
FT DOMAIN 497..858
FT /note="Glycosyl-hydrolase family 116 catalytic region"
FT /evidence="ECO:0000259|Pfam:PF04685"
SQ SEQUENCE 912 AA; 102627 MW; 5F2C5FEC2A5CD0B1 CRC64;
MVGPTLFHRR KHSWPPEEFI SKTTLQLLDF DSAAPPSHAW RRRLNCHANI LKEFTITFRE
AIKMVRLGIR LWSYVREEAS HGRKAPIDPF TKDNCRPSAS QGVPLGGMGS GSISRGFRGE
FKQWQITPGT CDPSPMMSNQ FSIFISRENV HKKYASVLAP GQHGSLGKLR DKGISSWGWN
LSGQHSTYHA LFPRAWTVYD GEPDPELKIS CRQISPFIPK NYRDSSLPAA VFVYTLVNTG
KQRAKVSLVF TWANSIGGTS HMSGGHVNEP FIGEDGVSGV LLHHKTGMGN PPVTFAISAC
ETQNVNVTVL PCFGLSEDSF FTAKDMWETM EKDGNFDQEN FNSGPSMPSS PGDTICAAVS
ASAWVEAHGK CTVSFALSWP SPKVKFSKGS TYDRRYTKFY GTSRRAAVDL VHDALTHYKR
WEEDIEAWQN PILRDDRLPE WYKFTLFNEL YFLVAGGTVW IDGKEQQNNL NNGDRNGIES
NGLFVETSQG DDEDDVGRFL YLEGVEYVMW NTYDVHFYAS YALLMLFPKI ELNIQRDFAK
AVLSEDTRKV KFLAEGNWGI RKVRGAVPHD LGMHDPWNEM NAYNIHDTSK WKDLNPKFVL
QVYRDFAATG DHQFGIDVWP AVRAAMEYME QFDRDNDDLI ENDGFPDQTY DTWTVHGVSA
YCGCLWLAAL QAAAAMALQV GDKFFAELCK NKFLNAKAAL EAKLWNGTYL NYDSGASSNS
KSIQTDQLAG QWYTASSGLP PVFEDFKIRS TLQKIFDFNV MKTKGGRMGA VNGMHPDGKV
DETCMQSREI WTGVTYAAAA TMILSGMEEQ GFTTAEGIFA AGWSEEGFGY WFQTPEGWTM
DGHYRSLIYM RPLAIWGMQW ALTVPKAILD GPKINMIDRV HMSPRSRRFS HNVKVVKHKA
KCFGNCKLSC SC
//