ID A0A078FG03_BRANA Unreviewed; 619 AA.
AC A0A078FG03;
DT 29-OCT-2014, integrated into UniProtKB/TrEMBL.
DT 29-OCT-2014, sequence version 1.
DT 27-MAR-2024, entry version 37.
DE RecName: Full=Endoglucanase {ECO:0000256|RuleBase:RU361166};
DE EC=3.2.1.4 {ECO:0000256|RuleBase:RU361166};
GN Name=BnaA03g46570D {ECO:0000313|EMBL:CDY10998.1};
GN ORFNames=DARMORV10_A03P55160.1 {ECO:0000313|EMBL:CAF2131123.1},
GN GSBRNA2T00048945001 {ECO:0000313|EMBL:CDY10998.1};
OS Brassica napus (Rape).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Brassiceae; Brassica.
OX NCBI_TaxID=3708 {ECO:0000313|EMBL:CDY10998.1, ECO:0000313|Proteomes:UP000028999};
RN [1] {ECO:0000313|EMBL:CDY10998.1, ECO:0000313|Proteomes:UP000028999}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Darmor-bzh {ECO:0000313|Proteomes:UP000028999};
RX PubMed=25146293; DOI=10.1126/science.1253435;
RA Chalhoub B., Denoeud F., Liu S., Parkin I.A., Tang H., Wang X., Chiquet J.,
RA Belcram H., Tong C., Samans B., Correa M., Da Silva C., Just J.,
RA Falentin C., Koh C.S., Le Clainche I., Bernard M., Bento P., Noel B.,
RA Labadie K., Alberti A., Charles M., Arnaud D., Guo H., Daviaud C.,
RA Alamery S., Jabbari K., Zhao M., Edger P.P., Chelaifa H., Tack D.,
RA Lassalle G., Mestiri I., Schnel N., Le Paslier M.C., Fan G., Renault V.,
RA Bayer P.E., Golicz A.A., Manoli S., Lee T.H., Thi V.H., Chalabi S., Hu Q.,
RA Fan C., Tollenaere R., Lu Y., Battail C., Shen J., Sidebottom C.H.,
RA Wang X., Canaguier A., Chauveau A., Berard A., Deniot G., Guan M., Liu Z.,
RA Sun F., Lim Y.P., Lyons E., Town C.D., Bancroft I., Wang X., Meng J.,
RA Ma J., Pires J.C., King G.J., Brunel D., Delourme R., Renard M., Aury J.M.,
RA Adams K.L., Batley J., Snowdon R.J., Tost J., Edwards D., Zhou Y., Hua W.,
RA Sharpe A.G., Paterson A.H., Guan C., Wincker P.;
RT "Plant genetics. Early allopolyploid evolution in the post-Neolithic
RT Brassica napus oilseed genome.";
RL Science 345:950-953(2014).
RN [2] {ECO:0000313|EMBL:CDY10998.1}
RP NUCLEOTIDE SEQUENCE.
RA Genoscope - CEA;
RL Submitted (JUN-2014) to the EMBL/GenBank/DDBJ databases.
RN [3] {ECO:0000313|EMBL:CAF2131123.1}
RP NUCLEOTIDE SEQUENCE.
RG Genoscope - CEA;
RA William W.;
RL Submitted (JAN-2021) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Endohydrolysis of (1->4)-beta-D-glucosidic linkages in
CC cellulose, lichenin and cereal beta-D-glucans.; EC=3.2.1.4;
CC Evidence={ECO:0000256|ARBA:ARBA00000966,
CC ECO:0000256|RuleBase:RU361166};
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 9 (cellulase E) family.
CC {ECO:0000256|ARBA:ARBA00007072, ECO:0000256|PROSITE-ProRule:PRU10059,
CC ECO:0000256|RuleBase:RU361166}.
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DR EMBL; HG994357; CAF2131123.1; -; Genomic_DNA.
DR EMBL; LK032008; CDY10998.1; -; Genomic_DNA.
DR RefSeq; XP_013741518.1; XM_013886064.1.
DR STRING; 3708.A0A078FG03; -.
DR PaxDb; 3708-A0A078FG03; -.
DR EnsemblPlants; CDY10998; CDY10998; GSBRNA2T00048945001.
DR GeneID; 106444607; -.
DR Gramene; CDY10998; CDY10998; GSBRNA2T00048945001.
DR KEGG; bna:106444607; -.
DR OMA; WDEYMWG; -.
DR OrthoDB; 655559at2759; -.
DR Proteomes; UP000028999; Unassembled WGS sequence.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR GO; GO:0008810; F:cellulase activity; IEA:UniProtKB-EC.
DR GO; GO:0030245; P:cellulose catabolic process; IEA:UniProtKB-KW.
DR Gene3D; 1.50.10.10; -; 1.
DR InterPro; IPR008928; 6-hairpin_glycosidase_sf.
DR InterPro; IPR012341; 6hp_glycosidase-like_sf.
DR InterPro; IPR001701; Glyco_hydro_9.
DR InterPro; IPR033126; Glyco_hydro_9_Asp/Glu_AS.
DR InterPro; IPR018221; Glyco_hydro_9_His_AS.
DR PANTHER; PTHR22298; ENDO-1,4-BETA-GLUCANASE; 1.
DR PANTHER; PTHR22298:SF116; ENDOGLUCANASE 21; 1.
DR Pfam; PF00759; Glyco_hydro_9; 1.
DR SUPFAM; SSF48208; Six-hairpin glycosidases; 1.
DR PROSITE; PS00592; GH9_2; 1.
DR PROSITE; PS00698; GH9_3; 1.
PE 3: Inferred from homology;
KW Carbohydrate metabolism {ECO:0000256|ARBA:ARBA00023277,
KW ECO:0000256|PROSITE-ProRule:PRU10059};
KW Cellulose degradation {ECO:0000256|ARBA:ARBA00023001,
KW ECO:0000256|RuleBase:RU361166};
KW Glycosidase {ECO:0000256|ARBA:ARBA00023295, ECO:0000256|PROSITE-
KW ProRule:PRU10059};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|PROSITE-
KW ProRule:PRU10059}; Membrane {ECO:0000256|SAM:Phobius};
KW Polysaccharide degradation {ECO:0000256|ARBA:ARBA00023326,
KW ECO:0000256|PROSITE-ProRule:PRU10059};
KW Reference proteome {ECO:0000313|Proteomes:UP000028999};
KW Transmembrane {ECO:0000256|SAM:Phobius};
KW Transmembrane helix {ECO:0000256|SAM:Phobius}.
FT TRANSMEM 71..93
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT DOMAIN 109..582
FT /note="Glycoside hydrolase family 9"
FT /evidence="ECO:0000259|Pfam:PF00759"
FT ACT_SITE 512
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU10059"
FT ACT_SITE 560
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU10060"
FT ACT_SITE 569
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU10060"
SQ SEQUENCE 619 AA; 69811 MW; BB5557276D644A01 CRC64;
MYGRDPWGGP LEINAADSIT DDDRSRNLQE LDRSTLRRPL DATQRSWLLG PPVRKKKKYV
DIGCMQVSRK IFLWTLGTLL VTTFLAGSIT MIVRHVPHHK HEKPQQDNYT LALNKTLLFF
NAQKSGKLPK KNNVSWRGNS CMKDVDEGEY PPRDVVGGYY DAGDTIKFSF PMSYAMTMLS
WSVIEYSAKY EAAGELDHVK EIIKWGTDYF LKTFNSSADT VDRMVSQVGF GDTRGGSVVH
NDHYCWMRPE DIDYKRPVHT CYSGCSDLAA EMAAALASAS IVFKDDRDYS KKLVHGAKTL
YNFANFNKKS YSKPYDESSE FYKSSLFWDE LLWGGAWLYY ATGNITYLDQ VTNDDMARHA
GAFWDGPYYG VPSWDNKLPG AQLLLTRLRL FLGPGYPYED ILRTFHNQTG VIMCSYLPYY
KKFKRTKGGL IKLNHGDPQP LQYAANAAFL AALFSDYLDA ADTPGWYCGP KFYSTDVLRD
FARSQIDYIL GKNPRKMSYV VGFGQRYPKH VHHRGASIPK SVKKESCKGG WKWRDTKKNN
PNTIVGAMVA GPDKHDGFHD LRTNYNYTEP TLAGNAGLVA ALVALSGERT FGAIDKNTMF
SAVPPLTPAT PPPPAPWTP
//
