ID A0A078G8V4_BRANA Unreviewed; 3116 AA.
AC A0A078G8V4;
DT 29-OCT-2014, integrated into UniProtKB/TrEMBL.
DT 29-OCT-2014, sequence version 1.
DT 27-MAR-2024, entry version 49.
DE RecName: Full=non-specific serine/threonine protein kinase {ECO:0000256|ARBA:ARBA00012513};
DE EC=2.7.11.1 {ECO:0000256|ARBA:ARBA00012513};
GN Name=BnaA06g20800D {ECO:0000313|EMBL:CDY21811.1};
GN ORFNames=GSBRNA2T00016363001 {ECO:0000313|EMBL:CDY21811.1};
OS Brassica napus (Rape).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Brassiceae; Brassica.
OX NCBI_TaxID=3708 {ECO:0000313|EMBL:CDY21811.1, ECO:0000313|Proteomes:UP000028999};
RN [1] {ECO:0000313|EMBL:CDY21811.1, ECO:0000313|Proteomes:UP000028999}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Darmor-bzh {ECO:0000313|Proteomes:UP000028999};
RX PubMed=25146293; DOI=10.1126/science.1253435;
RA Chalhoub B., Denoeud F., Liu S., Parkin I.A., Tang H., Wang X., Chiquet J.,
RA Belcram H., Tong C., Samans B., Correa M., Da Silva C., Just J.,
RA Falentin C., Koh C.S., Le Clainche I., Bernard M., Bento P., Noel B.,
RA Labadie K., Alberti A., Charles M., Arnaud D., Guo H., Daviaud C.,
RA Alamery S., Jabbari K., Zhao M., Edger P.P., Chelaifa H., Tack D.,
RA Lassalle G., Mestiri I., Schnel N., Le Paslier M.C., Fan G., Renault V.,
RA Bayer P.E., Golicz A.A., Manoli S., Lee T.H., Thi V.H., Chalabi S., Hu Q.,
RA Fan C., Tollenaere R., Lu Y., Battail C., Shen J., Sidebottom C.H.,
RA Wang X., Canaguier A., Chauveau A., Berard A., Deniot G., Guan M., Liu Z.,
RA Sun F., Lim Y.P., Lyons E., Town C.D., Bancroft I., Wang X., Meng J.,
RA Ma J., Pires J.C., King G.J., Brunel D., Delourme R., Renard M., Aury J.M.,
RA Adams K.L., Batley J., Snowdon R.J., Tost J., Edwards D., Zhou Y., Hua W.,
RA Sharpe A.G., Paterson A.H., Guan C., Wincker P.;
RT "Plant genetics. Early allopolyploid evolution in the post-Neolithic
RT Brassica napus oilseed genome.";
RL Science 345:950-953(2014).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + L-threonyl-[protein] = ADP + H(+) + O-phospho-L-
CC threonyl-[protein]; Xref=Rhea:RHEA:46608, Rhea:RHEA-COMP:11060,
CC Rhea:RHEA-COMP:11605, ChEBI:CHEBI:15378, ChEBI:CHEBI:30013,
CC ChEBI:CHEBI:30616, ChEBI:CHEBI:61977, ChEBI:CHEBI:456216;
CC EC=2.7.11.1; Evidence={ECO:0000256|ARBA:ARBA00000775};
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000256|ARBA:ARBA00004123}.
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DR EMBL; LK032124; CDY21811.1; -; Genomic_DNA.
DR STRING; 3708.A0A078G8V4; -.
DR PaxDb; 3708-A0A078G8V4; -.
DR EnsemblPlants; CDY21811; CDY21811; GSBRNA2T00016363001.
DR Gramene; CDY21811; CDY21811; GSBRNA2T00016363001.
DR OMA; FSHIWEV; -.
DR Proteomes; UP000028999; Unassembled WGS sequence.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0004674; F:protein serine/threonine kinase activity; IEA:UniProtKB-EC.
DR GO; GO:0006281; P:DNA repair; IEA:InterPro.
DR GO; GO:0016310; P:phosphorylation; IEA:UniProtKB-KW.
DR CDD; cd05171; PIKKc_ATM; 1.
DR Gene3D; 1.10.1070.11; Phosphatidylinositol 3-/4-kinase, catalytic domain; 1.
DR InterPro; IPR016024; ARM-type_fold.
DR InterPro; IPR038980; ATM_plant.
DR InterPro; IPR003152; FATC_dom.
DR InterPro; IPR011009; Kinase-like_dom_sf.
DR InterPro; IPR000403; PI3/4_kinase_cat_dom.
DR InterPro; IPR036940; PI3/4_kinase_cat_sf.
DR InterPro; IPR018936; PI3/4_kinase_CS.
DR InterPro; IPR003151; PIK-rel_kinase_FAT.
DR InterPro; IPR014009; PIK_FAT.
DR InterPro; IPR044107; PIKKc_ATM.
DR PANTHER; PTHR37079; SERINE/THREONINE-PROTEIN KINASE ATM; 1.
DR PANTHER; PTHR37079:SF4; SERINE_THREONINE-PROTEIN KINASE ATM; 1.
DR Pfam; PF02259; FAT; 1.
DR Pfam; PF02260; FATC; 1.
DR Pfam; PF00454; PI3_PI4_kinase; 1.
DR SMART; SM01343; FATC; 1.
DR SMART; SM00146; PI3Kc; 1.
DR SUPFAM; SSF48371; ARM repeat; 1.
DR SUPFAM; SSF56112; Protein kinase-like (PK-like); 1.
DR PROSITE; PS51189; FAT; 1.
DR PROSITE; PS51190; FATC; 1.
DR PROSITE; PS00915; PI3_4_KINASE_1; 1.
DR PROSITE; PS50290; PI3_4_KINASE_3; 1.
PE 4: Predicted;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840};
KW DNA damage {ECO:0000256|ARBA:ARBA00022763};
KW Kinase {ECO:0000256|ARBA:ARBA00022777}; Membrane {ECO:0000256|SAM:Phobius};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW Nucleus {ECO:0000256|ARBA:ARBA00023242};
KW Reference proteome {ECO:0000313|Proteomes:UP000028999};
KW Transferase {ECO:0000256|ARBA:ARBA00022679};
KW Transmembrane {ECO:0000256|SAM:Phobius};
KW Transmembrane helix {ECO:0000256|SAM:Phobius}.
FT TRANSMEM 1073..1098
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 1183..1202
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT DOMAIN 1966..2644
FT /note="FAT"
FT /evidence="ECO:0000259|PROSITE:PS51189"
FT DOMAIN 2750..3071
FT /note="PI3K/PI4K catalytic"
FT /evidence="ECO:0000259|PROSITE:PS50290"
FT DOMAIN 3084..3116
FT /note="FATC"
FT /evidence="ECO:0000259|PROSITE:PS51190"
SQ SEQUENCE 3116 AA; 352602 MW; FC7EC2498483D340 CRC64;
MITSRDVHEV VWKLSSDKAK TREDGVKLLN TWLEGDMSIN FCSFLSHNTA KLKLDQIPNA
ETWPFLVKNL LQCVSMEVSG SKRRIPKPTF AKALRVVIQR AEETKFPGVL SPLLSMAKTI
FTHVHDILSN TPSFHSEYGI ILRHLLEIKE YRFQMRKRTY SNLVMLYIER AEAGFWEKNS
GQHSQKEEAF RCILTLQSLL ENPPGDFSDD IRKEIVNGLI HIFSSARDEE KLSRKLIECV
NSFLLKDGPN IGSLSLEIHN AVQQFVFRCW LTTHDKNLKE ILAFYGRLQL NLTRGSSESS
SLVEQLLNVV TRELDLGSSS SSASWGDSTK DGTLSSYQNS LVELAAHVLY RAVVNTTRSS
LSEKRARRQH ITLRLVEALT EGKWLWCAAF GCLIRTHCTR INKDLLIYWF EAICTNFQRL
VEDASMRRSY DGLLWTLRSL QELSSGLLLP TATIDISKST VSSSELDRGW QLIWSSLIHG
LATFSSMTVI VDAVLVLLGS IISNNHINVG ILPQEVWDHQ LFRHIPSEPA LYFIACYFSR
MGCQGNLQDD LHLRRNLLRA VCGPLSLKGP LALNERMVRL LPAAALALCA GFTTTLPLPK
EHLPTPPSWD ACEVVNDVKM DDAEQERKFG LFECSVEVLT RVYSNPIKIS SYRVPDGVQL
PLVLRDPLLN DMEIYFLSII PEDSEKGPLS DIFMGCSLLC HFMHGSYTTR KGKGSTSFFL
KACQYLLESL DYAVEAVLKS LYDFQRLGPL GFGSDFNEKS SIIVSLRSLT SSPVFSNRRD
QNLLATSYDT VFHSLEDLLR SFAKVYGEYT EHSWNTKSDT VTSKSLALDP PEVGRIVDMD
LDLDVDTKEI DLITAGGKAV AGGPVSTGNW KLGMISLISC FSPVLQLPTW DVLYSIMEKE
CDPKVLENIL YHLCQLSCLT SMPKVYDLVI FLDDMLNKQV KNKRNCLNIV TALHVLLQNF
LSSGMDSSGL KPNSELSLLK EGESCQIFVQ LGAMVNKVSE CGLLGWFGRV RLISCICNFV
LLNPQIGQTM IERLLLMLND SDYRVRFVLA RQIGLLFQTW DGHEALFQDI CSVYHFSLLI
ASLMNFSILS SFGIILVTSS KEKLVTARDV LAAGPQPRPK METVIITLMH LAYHSENIEL
QAVFMMCAVS AIDPCQRELI IAALDNLSAQ LHYPSRFKYL EELLGPILFF WIACGVSLAA
LVETSQLFIL NAEPKYFIRF CSHWLLPALL LHEDNTNLDW VAKMASKPVS VLVKENFVPI
FSIGMGLHCS KTSECEKGAL VLQNSILYVG QITETERDKL IKRNMVSIVS FVLSRASSSP
EPPVPAFSRD TISRAIQTIV DGFLETADYP KNAAVIDNIN VFRPDRVFMF ITEIHYKMSA
ACHHRHTRHH LAALEELTII LGHRALVPSS LNYIFNLVGQ FIASPSLQDQ CCSIASCLLD
SFRSNPAKEI VSVLGDQLQF LVSKLVTCCI DAEANSKVSG SKSSQLVNLL HKLIANSESS
LHEDIRDLEP LPDMEIFRVI RESHIRRCEG YSPRNHLLKC ARRSCYLPPR FLSWSLQALH
HKLIATEASQ GESNLETGDS FWHSDDEIVN AVWTLVRVSA SDEADSMRLL ASDFLSRVGI
GDPHTVVFHL PGELGSMNDL QFVSHNKGSK VSSFTENGVS DETLISLLKI LKKYLLDDSV
KIIDVTSQTL RGILSTERGQ QALSSIDSCE RSLIEASFSS ALAFWSFHRL IFSQIIHKVE
NYLVNLILRV HGRGVNLDTV EKILLDSEKQ YKEKNLYVFY ELFLLLDAII CFHSENFSLE
KAEVWSTDNK NFDGWICQLV YCMISLCEDV PIRLCQNIAM LKAEISELLF PSVIVSLAGR
VGTDINLHEL ITSQVKEHIF VDSNKLTKSK QVMLNTLNEL RMCYILERST FSGQTKREKN
TKHSNYSSRS SSSAAKIRDV ETANGMAASI TANWDKVYWL SIDYLVAARS AVVCGAYLTA
AMYVEYWCEE KFGSLSLGDP DFSYHDMLPD HVEILVSAIT RINEPDSLYG VIHSNKLSAQ
ITTFEHEGNW SRALEYYDLQ ARSQKMVVPG SLPENQGVEH FQPTTSAQHS VFGEGEVQRQ
PFKGLIRSLQ QTGCMHVLDL YCRGLTSREG SFQYDPEFIE LQYEAAWRAG KWDFSLLYPQ
THSPPMQHVK NNNYHENLHG CLRALHEGDC NGFHGKLKDA KKELVLSISR ASEESTEFIY
SAVVKLQILY HLGLVWDLRW TTSSHESMHG YPVKQMACAD PVTPTMEQFS WLNKDWISIT
TQTQFHMNLL EPLVAFRRVL LQILGCEQCT MQHLLQSASL LRKGSKFSHA AASLHEFKFL
CAKSDGGQSV PDWLGRIEEA KILHAQGRHE VAISLANYIL QNYQLKEEAS DIYRVIGKWL
AETRSSNSRT ILDKYLKPAV SLAKNPSSEI SKRLVDRQSQ TWFHLAHYAD ALFKNYEERL
SSSEWQAAMR LRKHKVDRDN FLKLALEGYQ RCLQIGDKYD VRVLDIRLIY DRVTFETIYT
LYTFVFGIHV NTLPNKDIGV LSRLKDYLRD LRTRSVFHYV FRQVSMWFNL ASQKDVIDNM
LSTISEVQSY KFVPLVYQIA SRLGSSRDES GSNSFQSALV SLLRKMAIDH PYHTILQLLA
LANGDRIKDN QRSRNSFVVD IDKKVAAEHL LQDVSQHHGP MIRQMKQLVD IYIKLAELET
RREDTNKRVP LPREIRSVKQ LELVPVVTAT IPVDRSCQYN EGSFPSFRGL SDSVTVMNGI
NAPKVVECFG SDGRKYKQLA KSGNDDLRQD AVMEQFFGLV NTFLHNNRDT WKRRLAVRTY
KAYVSVVPFT PSAGVLEWVD GTIPLGDYLI GSSRAGGAHG RYGIGNWNYS KCREHMSNAK
DKRKAFMDVC TNFRPVMHYF FLEKFLHPAD WFLSEKVEYI SEAASLIGVL TFVGYIVGLG
DRHAMNILID QATAEVIHID LGVAFEQGLM LKTPERVPFR LTRDIIDGMG ITGVEGVFRR
CCEETLSVMR TNKEALLTIV EVFIHDPLYK WALSPLKALQ RQKETEDYDG VNLEGLQEEF
EGNKDAARAL MRVKQKLDGY EGGEMRSIHG QAQQLIQDAI DTDRLSHMFP GWGAWM
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