ID A0A078GH46_BRANA Unreviewed; 615 AA.
AC A0A078GH46;
DT 29-OCT-2014, integrated into UniProtKB/TrEMBL.
DT 29-OCT-2014, sequence version 1.
DT 27-MAR-2024, entry version 25.
DE RecName: Full=thioglucosidase {ECO:0000256|ARBA:ARBA00012250};
DE EC=3.2.1.147 {ECO:0000256|ARBA:ARBA00012250};
DE AltName: Full=Sinigrinase {ECO:0000256|ARBA:ARBA00032643};
DE AltName: Full=Thioglucosidase {ECO:0000256|ARBA:ARBA00032797};
GN Name=BnaA05g30850D {ECO:0000313|EMBL:CDY24686.1};
GN ORFNames=DARMORV10_A05P41510.1 {ECO:0000313|EMBL:CAF2102879.1},
GN GSBRNA2T00027067001 {ECO:0000313|EMBL:CDY24686.1};
OS Brassica napus (Rape).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Brassiceae; Brassica.
OX NCBI_TaxID=3708 {ECO:0000313|EMBL:CDY24686.1, ECO:0000313|Proteomes:UP000028999};
RN [1] {ECO:0000313|EMBL:CDY24686.1, ECO:0000313|Proteomes:UP000028999}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Darmor-bzh {ECO:0000313|Proteomes:UP000028999};
RX PubMed=25146293; DOI=10.1126/science.1253435;
RA Chalhoub B., Denoeud F., Liu S., Parkin I.A., Tang H., Wang X., Chiquet J.,
RA Belcram H., Tong C., Samans B., Correa M., Da Silva C., Just J.,
RA Falentin C., Koh C.S., Le Clainche I., Bernard M., Bento P., Noel B.,
RA Labadie K., Alberti A., Charles M., Arnaud D., Guo H., Daviaud C.,
RA Alamery S., Jabbari K., Zhao M., Edger P.P., Chelaifa H., Tack D.,
RA Lassalle G., Mestiri I., Schnel N., Le Paslier M.C., Fan G., Renault V.,
RA Bayer P.E., Golicz A.A., Manoli S., Lee T.H., Thi V.H., Chalabi S., Hu Q.,
RA Fan C., Tollenaere R., Lu Y., Battail C., Shen J., Sidebottom C.H.,
RA Wang X., Canaguier A., Chauveau A., Berard A., Deniot G., Guan M., Liu Z.,
RA Sun F., Lim Y.P., Lyons E., Town C.D., Bancroft I., Wang X., Meng J.,
RA Ma J., Pires J.C., King G.J., Brunel D., Delourme R., Renard M., Aury J.M.,
RA Adams K.L., Batley J., Snowdon R.J., Tost J., Edwards D., Zhou Y., Hua W.,
RA Sharpe A.G., Paterson A.H., Guan C., Wincker P.;
RT "Plant genetics. Early allopolyploid evolution in the post-Neolithic
RT Brassica napus oilseed genome.";
RL Science 345:950-953(2014).
RN [2] {ECO:0000313|EMBL:CDY24686.1}
RP NUCLEOTIDE SEQUENCE.
RA Genoscope - CEA;
RL Submitted (JUN-2014) to the EMBL/GenBank/DDBJ databases.
RN [3] {ECO:0000313|EMBL:CAF2102879.1}
RP NUCLEOTIDE SEQUENCE.
RG Genoscope - CEA;
RA William W.;
RL Submitted (JAN-2021) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Degradation of glucosinolates (glucose residue linked by a
CC thioglucoside bound to an amino acid derivative) to glucose, sulfate
CC and any of the products: thiocyanates, isothiocyanates, nitriles,
CC epithionitriles or oxazolidine-2-thiones.
CC {ECO:0000256|ARBA:ARBA00003014}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a thioglucoside + H2O = a sugar + a thiol.; EC=3.2.1.147;
CC Evidence={ECO:0000256|ARBA:ARBA00034026};
CC -!- SUBUNIT: Homodimer. {ECO:0000256|ARBA:ARBA00011738}.
CC -!- SUBCELLULAR LOCATION: Vacuole {ECO:0000256|ARBA:ARBA00004116}.
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 1 family.
CC {ECO:0000256|ARBA:ARBA00010838, ECO:0000256|RuleBase:RU003690}.
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DR EMBL; HG994359; CAF2102879.1; -; Genomic_DNA.
DR EMBL; LK032162; CDY24686.1; -; Genomic_DNA.
DR RefSeq; XP_013750665.1; XM_013895211.1.
DR STRING; 3708.A0A078GH46; -.
DR PaxDb; 3708-A0A078GH46; -.
DR EnsemblPlants; CDY24686; CDY24686; GSBRNA2T00027067001.
DR GeneID; 106452997; -.
DR Gramene; CDY24686; CDY24686; GSBRNA2T00027067001.
DR KEGG; bna:106452997; -.
DR OMA; ETSCDDN; -.
DR OrthoDB; 359146at2759; -.
DR Proteomes; UP000028999; Unassembled WGS sequence.
DR GO; GO:0005773; C:vacuole; IEA:UniProtKB-SubCell.
DR GO; GO:0008422; F:beta-glucosidase activity; IBA:GO_Central.
DR GO; GO:0102799; F:glucosinolate glucohydrolase activity; IEA:UniProtKB-EC.
DR GO; GO:0019137; F:thioglucosidase activity; IEA:UniProtKB-EC.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR Gene3D; 3.20.20.80; Glycosidases; 1.
DR InterPro; IPR001360; Glyco_hydro_1.
DR InterPro; IPR018120; Glyco_hydro_1_AS.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR PANTHER; PTHR10353:SF209; GALACTOLIPID GALACTOSYLTRANSFERASE SFR2, CHLOROPLASTIC; 1.
DR PANTHER; PTHR10353; GLYCOSYL HYDROLASE; 1.
DR Pfam; PF00232; Glyco_hydro_1; 2.
DR PRINTS; PR00131; GLHYDRLASE1.
DR SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR PROSITE; PS00572; GLYCOSYL_HYDROL_F1_1; 1.
PE 3: Inferred from homology;
KW Reference proteome {ECO:0000313|Proteomes:UP000028999};
KW Vacuole {ECO:0000256|ARBA:ARBA00022554}.
FT ACT_SITE 428
FT /note="Nucleophile"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU10055"
SQ SEQUENCE 615 AA; 69932 MW; BFE97E519A539328 CRC64;
MSILTLLVKI AGLLGTITVG ANAVSYSRFR RRNLRNFRSP IDESKEVLAD FTSQEHNEGK
FFFGLATAPA HAEDDLDDAW IRFAKETPCS AEDEEDKKAK RKKKVKLAVG AITKGLAKNT
QGKEDNTVAD STPTKNVAAW HNTPHAEVRL KFWSDPDQEV KLAKDTGVTV FRMGVDWCRI
MPKEPTKGIE EAVDYEALEH YKWILNRVRS NGMKVMLTLF HHSLPPWAAD YGGWKMEKTV
DYFMDFTRLV VDSMFDLVDS WITFNEPHVF AMLTYMAGTW PGNTPDFLEM ATSTLPMGVF
HRVMHWMAVA HSKAYDYIHA KTSLEKPLVG VAHHVSFMRP YGLFDVGSVT FSNSLTMFSY
IDSICEKLDF IGINYYGQEA VCGVGLKLVE TDEYSESGRG VYPDGLYRVL LMFHQRYKHL
NVPFIVTENG VSDETDVIRR PYMIEHLLAL YAAMLKGVPV LGYIFWTISD NWEWADGYGP
KFGLVAVDRA NNLARTVRPS YHLFTKIVKS GKITRNDRSL AWEELQKAAK AGKVRPFYRA
VDNHGLMYAD GLDKPQGRQF VDRDWRFGHY QVDGLQDPLS RLARALLIWP AITKKKIRKV
KVKHTDESGL ALQPA
//
