ID A0A078H4S9_BRANA Unreviewed; 575 AA.
AC A0A078H4S9;
DT 29-OCT-2014, integrated into UniProtKB/TrEMBL.
DT 29-OCT-2014, sequence version 1.
DT 27-MAR-2024, entry version 45.
DE RecName: Full=asparagine--tRNA ligase {ECO:0000256|ARBA:ARBA00012816};
DE EC=6.1.1.22 {ECO:0000256|ARBA:ARBA00012816};
GN Name=BnaC09g33230D {ECO:0000313|EMBL:CDY32876.1};
GN ORFNames=DARMORV10_C09P48270.1 {ECO:0000313|EMBL:CAF1764945.1},
GN GSBRNA2T00053005001 {ECO:0000313|EMBL:CDY32876.1};
OS Brassica napus (Rape).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Brassiceae; Brassica.
OX NCBI_TaxID=3708 {ECO:0000313|EMBL:CDY32876.1, ECO:0000313|Proteomes:UP000028999};
RN [1] {ECO:0000313|EMBL:CDY32876.1, ECO:0000313|Proteomes:UP000028999}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Darmor-bzh {ECO:0000313|Proteomes:UP000028999};
RX PubMed=25146293; DOI=10.1126/science.1253435;
RA Chalhoub B., Denoeud F., Liu S., Parkin I.A., Tang H., Wang X., Chiquet J.,
RA Belcram H., Tong C., Samans B., Correa M., Da Silva C., Just J.,
RA Falentin C., Koh C.S., Le Clainche I., Bernard M., Bento P., Noel B.,
RA Labadie K., Alberti A., Charles M., Arnaud D., Guo H., Daviaud C.,
RA Alamery S., Jabbari K., Zhao M., Edger P.P., Chelaifa H., Tack D.,
RA Lassalle G., Mestiri I., Schnel N., Le Paslier M.C., Fan G., Renault V.,
RA Bayer P.E., Golicz A.A., Manoli S., Lee T.H., Thi V.H., Chalabi S., Hu Q.,
RA Fan C., Tollenaere R., Lu Y., Battail C., Shen J., Sidebottom C.H.,
RA Wang X., Canaguier A., Chauveau A., Berard A., Deniot G., Guan M., Liu Z.,
RA Sun F., Lim Y.P., Lyons E., Town C.D., Bancroft I., Wang X., Meng J.,
RA Ma J., Pires J.C., King G.J., Brunel D., Delourme R., Renard M., Aury J.M.,
RA Adams K.L., Batley J., Snowdon R.J., Tost J., Edwards D., Zhou Y., Hua W.,
RA Sharpe A.G., Paterson A.H., Guan C., Wincker P.;
RT "Plant genetics. Early allopolyploid evolution in the post-Neolithic
RT Brassica napus oilseed genome.";
RL Science 345:950-953(2014).
RN [2] {ECO:0000313|EMBL:CDY32876.1}
RP NUCLEOTIDE SEQUENCE.
RA Genoscope - CEA;
RL Submitted (JUN-2014) to the EMBL/GenBank/DDBJ databases.
RN [3] {ECO:0000313|EMBL:CAF1764945.1}
RP NUCLEOTIDE SEQUENCE.
RG Genoscope - CEA;
RA William W.;
RL Submitted (JAN-2021) to the EMBL/GenBank/DDBJ databases.
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DR EMBL; HG994373; CAF1764945.1; -; Genomic_DNA.
DR EMBL; LK032301; CDY32876.1; -; Genomic_DNA.
DR RefSeq; XP_013729126.1; XM_013873672.1.
DR STRING; 3708.A0A078H4S9; -.
DR PaxDb; 3708-A0A078H4S9; -.
DR EnsemblPlants; CDY32876; CDY32876; GSBRNA2T00053005001.
DR GeneID; 106432830; -.
DR Gramene; CDY32876; CDY32876; GSBRNA2T00053005001.
DR KEGG; bna:106432830; -.
DR OMA; IRTCLAN; -.
DR OrthoDB; 347413at2759; -.
DR Proteomes; UP000028999; Unassembled WGS sequence.
DR GO; GO:0005739; C:mitochondrion; IBA:GO_Central.
DR GO; GO:0004816; F:asparagine-tRNA ligase activity; IBA:GO_Central.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0006421; P:asparaginyl-tRNA aminoacylation; IBA:GO_Central.
DR CDD; cd00776; AsxRS_core; 1.
DR CDD; cd04318; EcAsnRS_like_N; 1.
DR Gene3D; 2.40.50.140; Nucleic acid-binding proteins; 1.
DR Gene3D; 1.10.287.10; S15/NS1, RNA-binding; 1.
DR HAMAP; MF_00534; Asn_tRNA_synth; 1.
DR InterPro; IPR004364; Aa-tRNA-synt_II.
DR InterPro; IPR006195; aa-tRNA-synth_II.
DR InterPro; IPR045864; aa-tRNA-synth_II/BPL/LPL.
DR InterPro; IPR004522; Asn-tRNA-ligase.
DR InterPro; IPR002312; Asp/Asn-tRNA-synth_IIb.
DR InterPro; IPR012340; NA-bd_OB-fold.
DR InterPro; IPR004365; NA-bd_OB_tRNA.
DR InterPro; IPR000738; WHEP-TRS_dom.
DR NCBIfam; TIGR00457; asnS; 1.
DR PANTHER; PTHR22594:SF53; ASPARAGINE--TRNA LIGASE; 1.
DR PANTHER; PTHR22594; ASPARTYL/LYSYL-TRNA SYNTHETASE; 1.
DR Pfam; PF00152; tRNA-synt_2; 2.
DR Pfam; PF01336; tRNA_anti-codon; 1.
DR PRINTS; PR01042; TRNASYNTHASP.
DR SMART; SM00991; WHEP-TRS; 1.
DR SUPFAM; SSF55681; Class II aaRS and biotin synthetases; 1.
DR SUPFAM; SSF50249; Nucleic acid-binding proteins; 1.
DR PROSITE; PS50862; AA_TRNA_LIGASE_II; 1.
DR PROSITE; PS51185; WHEP_TRS_2; 1.
PE 3: Inferred from homology;
KW Aminoacyl-tRNA synthetase {ECO:0000256|ARBA:ARBA00023146};
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840};
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125};
KW Ligase {ECO:0000256|ARBA:ARBA00022598};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW Protein biosynthesis {ECO:0000256|ARBA:ARBA00022917};
KW Reference proteome {ECO:0000313|Proteomes:UP000028999}.
FT DOMAIN 239..295
FT /note="WHEP-TRS"
FT /evidence="ECO:0000259|PROSITE:PS51185"
FT DOMAIN 332..565
FT /note="Aminoacyl-transfer RNA synthetases class-II family
FT profile"
FT /evidence="ECO:0000259|PROSITE:PS50862"
SQ SEQUENCE 575 AA; 63627 MW; CE9B989C70B30942 CRC64;
MGGDIPPPVD ELAAVSLTND EASSSTVQKA RFSDRVRIQS ILGRPDGGAG LAGQKVRISG
WVKTGREQGK GAFAFLEVND GSCPANLQVM VDASVSDLSK LIATGTCVTV DGYLKLPPEG
KGTKQKIELS VVEVIDLGTV DPGTYPIPKT KLTLERLREF LHLRARTNSI SAIARIRHAL
AIATHTFFDE EGFLYVQTPI ITTSDCEGAG EMFQVTTLIS TTEKLERELI ENPPPTEADV
EAARVVVKER GEAVAELKAA KASKEAILAS VAELNDAKAN FAATEARSRL KPGLPKVDGK
IDYSQDFFGR QAFLTVSGQL QVETYACGLS DVYTFGPTFR AENSHTSRHL AEFWMVEPEL
AFADLEDDMN CAEAYVKYMC KWLLEKRYDD MELMAKNFDK GCIDRLKLVA STPFGRLTYT
EAIKILEEAV AKGKNFDNAV EWGIDLASEH ERYLTEVVFQ KPLIVYNYPK GIKAFYMRLN
DDGKTVAAMD VLVPKVGELI GGSQREERID VIMERLEEIG LPVEPYEWYL DLRRYGTAKH
SGFGLGFERM VLFATGMDNI RDVIPFPRYP GRADL
//
