ID A0A078H9S8_BRANA Unreviewed; 620 AA.
AC A0A078H9S8;
DT 29-OCT-2014, integrated into UniProtKB/TrEMBL.
DT 29-OCT-2014, sequence version 1.
DT 24-JAN-2024, entry version 36.
DE RecName: Full=Endoglucanase {ECO:0000256|RuleBase:RU361166};
DE EC=3.2.1.4 {ECO:0000256|RuleBase:RU361166};
GN Name=BnaA09g11640D {ECO:0000313|EMBL:CDY34422.1};
GN ORFNames=DARMORV10_A09P14340.1 {ECO:0000313|EMBL:CAF2039315.1},
GN GSBRNA2T00056494001 {ECO:0000313|EMBL:CDY34422.1};
OS Brassica napus (Rape).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Brassiceae; Brassica.
OX NCBI_TaxID=3708 {ECO:0000313|EMBL:CDY34422.1, ECO:0000313|Proteomes:UP000028999};
RN [1] {ECO:0000313|EMBL:CDY34422.1, ECO:0000313|Proteomes:UP000028999}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Darmor-bzh {ECO:0000313|Proteomes:UP000028999};
RX PubMed=25146293; DOI=10.1126/science.1253435;
RA Chalhoub B., Denoeud F., Liu S., Parkin I.A., Tang H., Wang X., Chiquet J.,
RA Belcram H., Tong C., Samans B., Correa M., Da Silva C., Just J.,
RA Falentin C., Koh C.S., Le Clainche I., Bernard M., Bento P., Noel B.,
RA Labadie K., Alberti A., Charles M., Arnaud D., Guo H., Daviaud C.,
RA Alamery S., Jabbari K., Zhao M., Edger P.P., Chelaifa H., Tack D.,
RA Lassalle G., Mestiri I., Schnel N., Le Paslier M.C., Fan G., Renault V.,
RA Bayer P.E., Golicz A.A., Manoli S., Lee T.H., Thi V.H., Chalabi S., Hu Q.,
RA Fan C., Tollenaere R., Lu Y., Battail C., Shen J., Sidebottom C.H.,
RA Wang X., Canaguier A., Chauveau A., Berard A., Deniot G., Guan M., Liu Z.,
RA Sun F., Lim Y.P., Lyons E., Town C.D., Bancroft I., Wang X., Meng J.,
RA Ma J., Pires J.C., King G.J., Brunel D., Delourme R., Renard M., Aury J.M.,
RA Adams K.L., Batley J., Snowdon R.J., Tost J., Edwards D., Zhou Y., Hua W.,
RA Sharpe A.G., Paterson A.H., Guan C., Wincker P.;
RT "Plant genetics. Early allopolyploid evolution in the post-Neolithic
RT Brassica napus oilseed genome.";
RL Science 345:950-953(2014).
RN [2] {ECO:0000313|EMBL:CDY34422.1}
RP NUCLEOTIDE SEQUENCE.
RA Genoscope - CEA;
RL Submitted (JUN-2014) to the EMBL/GenBank/DDBJ databases.
RN [3] {ECO:0000313|EMBL:CAF2039315.1}
RP NUCLEOTIDE SEQUENCE.
RG Genoscope - CEA;
RA William W.;
RL Submitted (JAN-2021) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Endohydrolysis of (1->4)-beta-D-glucosidic linkages in
CC cellulose, lichenin and cereal beta-D-glucans.; EC=3.2.1.4;
CC Evidence={ECO:0000256|ARBA:ARBA00000966,
CC ECO:0000256|RuleBase:RU361166};
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000256|ARBA:ARBA00004613}.
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 9 (cellulase E) family.
CC {ECO:0000256|ARBA:ARBA00007072, ECO:0000256|PROSITE-ProRule:PRU10059,
CC ECO:0000256|RuleBase:RU361166}.
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DR EMBL; HG994363; CAF2039315.1; -; Genomic_DNA.
DR EMBL; LK032333; CDY34422.1; -; Genomic_DNA.
DR STRING; 3708.A0A078H9S8; -.
DR PaxDb; 3708-A0A078H9S8; -.
DR EnsemblPlants; CDY34422; CDY34422; GSBRNA2T00056494001.
DR Gramene; CDY34422; CDY34422; GSBRNA2T00056494001.
DR OMA; WLYQASD; -.
DR Proteomes; UP000028999; Unassembled WGS sequence.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0030246; F:carbohydrate binding; IEA:InterPro.
DR GO; GO:0008810; F:cellulase activity; IEA:UniProtKB-EC.
DR GO; GO:0030245; P:cellulose catabolic process; IEA:UniProtKB-KW.
DR Gene3D; 1.50.10.10; -; 1.
DR InterPro; IPR008928; 6-hairpin_glycosidase_sf.
DR InterPro; IPR012341; 6hp_glycosidase-like_sf.
DR InterPro; IPR019028; CBM_49.
DR InterPro; IPR001701; Glyco_hydro_9.
DR InterPro; IPR033126; Glyco_hydro_9_Asp/Glu_AS.
DR InterPro; IPR018221; Glyco_hydro_9_His_AS.
DR PANTHER; PTHR22298; ENDO-1,4-BETA-GLUCANASE; 1.
DR PANTHER; PTHR22298:SF19; ENDOGLUCANASE 19-RELATED; 1.
DR Pfam; PF09478; CBM49; 1.
DR Pfam; PF00759; Glyco_hydro_9; 1.
DR SMART; SM01063; CBM49; 1.
DR SUPFAM; SSF48208; Six-hairpin glycosidases; 1.
DR PROSITE; PS00592; GH9_2; 1.
DR PROSITE; PS00698; GH9_3; 1.
PE 3: Inferred from homology;
KW Carbohydrate metabolism {ECO:0000256|ARBA:ARBA00023277,
KW ECO:0000256|PROSITE-ProRule:PRU10059};
KW Cellulose degradation {ECO:0000256|ARBA:ARBA00023001,
KW ECO:0000256|RuleBase:RU361166};
KW Glycoprotein {ECO:0000256|ARBA:ARBA00023180};
KW Glycosidase {ECO:0000256|ARBA:ARBA00023295, ECO:0000256|PROSITE-
KW ProRule:PRU10059};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|PROSITE-
KW ProRule:PRU10059};
KW Polysaccharide degradation {ECO:0000256|ARBA:ARBA00023326,
KW ECO:0000256|PROSITE-ProRule:PRU10059};
KW Reference proteome {ECO:0000313|Proteomes:UP000028999};
KW Secreted {ECO:0000256|ARBA:ARBA00022525};
KW Signal {ECO:0000256|RuleBase:RU361166}.
FT SIGNAL 1..21
FT /evidence="ECO:0000256|RuleBase:RU361166"
FT CHAIN 22..620
FT /note="Endoglucanase"
FT /evidence="ECO:0000256|RuleBase:RU361166"
FT /id="PRO_5041001405"
FT DOMAIN 528..608
FT /note="Carbohydrate binding"
FT /evidence="ECO:0000259|SMART:SM01063"
FT ACT_SITE 410
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU10059"
FT ACT_SITE 462
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU10060"
FT ACT_SITE 471
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU10060"
SQ SEQUENCE 620 AA; 68402 MW; 82C99A958D641B76 CRC64;
MEKFASVAAL LLLLSFPVAF SGHDYGQALS KSLLFFEAQR SGVLPRNQRV TWRSHSGLTD
GKSSGVNLVG GYYDAGDNVK FGLPMAFTVT MMAWSVIEYG NQLAANGELG HSIDAIKWGT
DYFIKAHPEP NVLYGEVGDG NTDHYCWQRP EEMTTDRKAY RIDPSNPGSD LAGETAAAMA
AASIVFRRSN PAYSRLLLTH AYQLFDFADK YRGKYDSSIT VARKYYGSVS GYNDELLWAA
AWLYQASNNQ FYLDYLGRNG DSMGGTGWSM TEFGWDVKYA GVQTLVAKFL MQGKAGRHTA
VFQKFQQKAD FFMCSLLGKG SRNIQKTPGG LIFRQRWNNM QFVTSASFLT TVYSDYLTSS
RSNLRCSAGN VAPSQLLSFA KSQVDYILGD NPRATSYMVG YGNNFPQRVH HRGSSIVSYK
VDRSFVTCRG GYATWFSRKG SDPNLLTGAI VGGPDAYDNF ADRRDNYEQT EPATYNNAPL
LGVLARLSSG HSGYSQLLPA VPAPVVVRRP MPIRKPRVTS PVRASGPVAI VQKMTGSWVS
KGRTYYRYST TVINKSPRAL KSLNLSIKNL YGPIWGLSRS GNSFGLPSWM HSLQSGKSLE
FVYIHSTSPA NVAVSSYTLA
//
