ID A0A078IEP9_BRANA Unreviewed; 667 AA.
AC A0A078IEP9;
DT 29-OCT-2014, integrated into UniProtKB/TrEMBL.
DT 29-OCT-2014, sequence version 1.
DT 27-MAR-2024, entry version 30.
DE RecName: Full=Long-chain-fatty-acid--CoA ligase {ECO:0000256|ARBA:ARBA00026121, ECO:0000256|RuleBase:RU369030};
DE EC=6.2.1.3 {ECO:0000256|ARBA:ARBA00026121, ECO:0000256|RuleBase:RU369030};
GN Name=BnaA01g13470D {ECO:0000313|EMBL:CDY47618.1};
GN ORFNames=GSBRNA2T00087831001 {ECO:0000313|EMBL:CDY47618.1};
OS Brassica napus (Rape).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Brassiceae; Brassica.
OX NCBI_TaxID=3708 {ECO:0000313|EMBL:CDY47618.1, ECO:0000313|Proteomes:UP000028999};
RN [1] {ECO:0000313|EMBL:CDY47618.1, ECO:0000313|Proteomes:UP000028999}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Darmor-bzh {ECO:0000313|Proteomes:UP000028999};
RX PubMed=25146293; DOI=10.1126/science.1253435;
RA Chalhoub B., Denoeud F., Liu S., Parkin I.A., Tang H., Wang X., Chiquet J.,
RA Belcram H., Tong C., Samans B., Correa M., Da Silva C., Just J.,
RA Falentin C., Koh C.S., Le Clainche I., Bernard M., Bento P., Noel B.,
RA Labadie K., Alberti A., Charles M., Arnaud D., Guo H., Daviaud C.,
RA Alamery S., Jabbari K., Zhao M., Edger P.P., Chelaifa H., Tack D.,
RA Lassalle G., Mestiri I., Schnel N., Le Paslier M.C., Fan G., Renault V.,
RA Bayer P.E., Golicz A.A., Manoli S., Lee T.H., Thi V.H., Chalabi S., Hu Q.,
RA Fan C., Tollenaere R., Lu Y., Battail C., Shen J., Sidebottom C.H.,
RA Wang X., Canaguier A., Chauveau A., Berard A., Deniot G., Guan M., Liu Z.,
RA Sun F., Lim Y.P., Lyons E., Town C.D., Bancroft I., Wang X., Meng J.,
RA Ma J., Pires J.C., King G.J., Brunel D., Delourme R., Renard M., Aury J.M.,
RA Adams K.L., Batley J., Snowdon R.J., Tost J., Edwards D., Zhou Y., Hua W.,
RA Sharpe A.G., Paterson A.H., Guan C., Wincker P.;
RT "Plant genetics. Early allopolyploid evolution in the post-Neolithic
RT Brassica napus oilseed genome.";
RL Science 345:950-953(2014).
CC -!- FUNCTION: Catalyzes the conversion of long-chain fatty acids to their
CC active form acyl-CoAs for both synthesis of cellular lipids, and
CC degradation via beta-oxidation. {ECO:0000256|RuleBase:RU369030}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a long-chain fatty acid + ATP + CoA = a long-chain fatty acyl-
CC CoA + AMP + diphosphate; Xref=Rhea:RHEA:15421, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:33019, ChEBI:CHEBI:57287, ChEBI:CHEBI:57560,
CC ChEBI:CHEBI:83139, ChEBI:CHEBI:456215; EC=6.2.1.3;
CC Evidence={ECO:0000256|RuleBase:RU369030};
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000256|ARBA:ARBA00001946};
CC -!- PATHWAY: Lipid metabolism; fatty acid metabolism.
CC {ECO:0000256|ARBA:ARBA00004872, ECO:0000256|RuleBase:RU369030}.
CC -!- SIMILARITY: Belongs to the ATP-dependent AMP-binding enzyme family.
CC {ECO:0000256|ARBA:ARBA00006432, ECO:0000256|RuleBase:RU369030}.
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DR EMBL; LK032726; CDY47618.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A078IEP9; -.
DR SMR; A0A078IEP9; -.
DR STRING; 3708.A0A078IEP9; -.
DR PaxDb; 3708-A0A078IEP9; -.
DR EnsemblPlants; CDY47618; CDY47618; GSBRNA2T00087831001.
DR Gramene; CDY47618; CDY47618; GSBRNA2T00087831001.
DR OMA; SAKEAKM; -.
DR OrthoDB; 22305at2759; -.
DR UniPathway; UPA00199; -.
DR Proteomes; UP000028999; Unassembled WGS sequence.
DR GO; GO:0005783; C:endoplasmic reticulum; IBA:GO_Central.
DR GO; GO:0016020; C:membrane; IBA:GO_Central.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0004467; F:long-chain fatty acid-CoA ligase activity; IBA:GO_Central.
DR CDD; cd05927; LC-FACS_euk; 1.
DR Gene3D; 3.40.50.12780; N-terminal domain of ligase-like; 1.
DR InterPro; IPR020845; AMP-binding_CS.
DR InterPro; IPR000873; AMP-dep_Synth/Lig_com.
DR InterPro; IPR042099; ANL_N_sf.
DR InterPro; IPR045311; LC-FACS_euk.
DR PANTHER; PTHR43272:SF105; LONG CHAIN ACYL-COA SYNTHETASE 4; 1.
DR PANTHER; PTHR43272; LONG-CHAIN-FATTY-ACID--COA LIGASE; 1.
DR Pfam; PF00501; AMP-binding; 1.
DR SUPFAM; SSF56801; Acetyl-CoA synthetase-like; 1.
DR PROSITE; PS00455; AMP_BINDING; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|RuleBase:RU369030};
KW Fatty acid metabolism {ECO:0000256|RuleBase:RU369030};
KW Ligase {ECO:0000256|RuleBase:RU369030};
KW Lipid metabolism {ECO:0000256|RuleBase:RU369030};
KW Nucleotide-binding {ECO:0000256|RuleBase:RU369030};
KW Reference proteome {ECO:0000313|Proteomes:UP000028999}.
FT DOMAIN 55..484
FT /note="AMP-dependent synthetase/ligase"
FT /evidence="ECO:0000259|Pfam:PF00501"
SQ SEQUENCE 667 AA; 74463 MW; CB0AE817BD41EE33 CRC64;
MTQQLEKFIF QVEEGREGSD GRPSVGPVYR SVFAKDGFPE PIEGMDSCWD VFRMSVEKYP
NNPMLGRREI VDGKPGKYVW QTYQEVYDIV IKLGNSLRSC GVEEQAKCGI YGANSPEWII
SMEACNAHGL YCVPLYDTLG AGAVEFIITH SEVSIVFVEE KKISELFKTC PKATEYMKTV
VSFGGVTREQ KEAAETFGLV IYAWDEFLKL GEGKQFDLPI KKKSDICTIM YTSGTTGDPK
GVMISNESIV TLIAGVIRLL KSANEALTVK DVYLSYLPLA HIFDRVIEEC FIQHGAAIGF
WRGDVKLLIE DLGELKPTIF CAVPRVLDRV YSGLQQKLAA GGFFKKFMFD SAFSFKYGHM
KKGQSHVEAS PLCDKLVFSK VKQGLGGNVR IILSGAAPLA SHVESFLRVV ACCHVLQGYG
LTESCAGTFV SLPDRLDMLG TVGPPVPNVD IRLESVPEME YDALASTPRG EICIRGKTLF
SGYYKREDLT KEVLIDGWLH TGDVGEWQPN GSMKIIDRKK NIFKLSQGEY VAVENLENIY
GEVQAVDSVW VYGNSFESFL IAVANPNQHI LEKWAAENGV SGDYNALCQN AKAKEFILGE
LVKMGKEKKM KGFEIIKAVH LDPVPFDMER DLLTPTYKKK RPQLLKHYQS VIDEMYKTTN
ESLASRK
//
