ID A0A087ASQ3_9BIFI Unreviewed; 695 AA.
AC A0A087ASQ3;
DT 29-OCT-2014, integrated into UniProtKB/TrEMBL.
DT 29-OCT-2014, sequence version 1.
DT 24-JAN-2024, entry version 28.
DE RecName: Full=Beta-galactosidase {ECO:0000256|ARBA:ARBA00012756, ECO:0000256|PIRNR:PIRNR001084};
DE Short=Beta-gal {ECO:0000256|PIRNR:PIRNR001084};
DE EC=3.2.1.23 {ECO:0000256|ARBA:ARBA00012756, ECO:0000256|PIRNR:PIRNR001084};
GN ORFNames=BIGA_0327 {ECO:0000313|EMBL:KFI61803.1};
OS Bifidobacterium pullorum subsp. gallinarum.
OC Bacteria; Actinomycetota; Actinomycetes; Bifidobacteriales;
OC Bifidobacteriaceae; Bifidobacterium.
OX NCBI_TaxID=78344 {ECO:0000313|EMBL:KFI61803.1, ECO:0000313|Proteomes:UP000029046};
RN [1] {ECO:0000313|EMBL:KFI61803.1, ECO:0000313|Proteomes:UP000029046}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=LMG 11586 {ECO:0000313|EMBL:KFI61803.1,
RC ECO:0000313|Proteomes:UP000029046};
RA Ventura M., Milani C., Lugli G.A.;
RT "Genomics of Bifidobacteria.";
RL Submitted (MAR-2014) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Hydrolysis of terminal non-reducing beta-D-galactose residues
CC in beta-D-galactosides.; EC=3.2.1.23;
CC Evidence={ECO:0000256|ARBA:ARBA00001412,
CC ECO:0000256|PIRNR:PIRNR001084};
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 42 family.
CC {ECO:0000256|ARBA:ARBA00005940, ECO:0000256|PIRNR:PIRNR001084}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KFI61803.1}.
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DR EMBL; JGYX01000001; KFI61803.1; -; Genomic_DNA.
DR RefSeq; WP_033507957.1; NZ_JGYX01000001.1.
DR AlphaFoldDB; A0A087ASQ3; -.
DR eggNOG; COG1874; Bacteria.
DR OrthoDB; 9800974at2; -.
DR Proteomes; UP000029046; Unassembled WGS sequence.
DR GO; GO:0009341; C:beta-galactosidase complex; IEA:InterPro.
DR GO; GO:0004565; F:beta-galactosidase activity; IEA:UniProtKB-EC.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR CDD; cd03143; A4_beta-galactosidase_middle_domain; 1.
DR Gene3D; 3.40.50.880; -; 1.
DR Gene3D; 3.20.20.80; Glycosidases; 1.
DR Gene3D; 2.60.40.1180; Golgi alpha-mannosidase II; 1.
DR InterPro; IPR013738; Beta_galactosidase_Trimer.
DR InterPro; IPR029062; Class_I_gatase-like.
DR InterPro; IPR003476; Glyco_hydro_42.
DR InterPro; IPR013529; Glyco_hydro_42_N.
DR InterPro; IPR013780; Glyco_hydro_b.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR PANTHER; PTHR36447; BETA-GALACTOSIDASE GANA; 1.
DR PANTHER; PTHR36447:SF1; BETA-GALACTOSIDASE GANA; 1.
DR Pfam; PF02449; Glyco_hydro_42; 1.
DR Pfam; PF08532; Glyco_hydro_42M; 1.
DR PIRSF; PIRSF001084; B-galactosidase; 1.
DR SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR SUPFAM; SSF52317; Class I glutamine amidotransferase-like; 1.
PE 3: Inferred from homology;
KW Glycosidase {ECO:0000256|PIRNR:PIRNR001084, ECO:0000313|EMBL:KFI61803.1};
KW Hydrolase {ECO:0000256|PIRNR:PIRNR001084, ECO:0000313|EMBL:KFI61803.1};
KW Reference proteome {ECO:0000313|Proteomes:UP000029046}.
FT DOMAIN 25..397
FT /note="Glycoside hydrolase family 42 N-terminal"
FT /evidence="ECO:0000259|Pfam:PF02449"
FT DOMAIN 411..620
FT /note="Beta-galactosidase trimerisation"
FT /evidence="ECO:0000259|Pfam:PF08532"
FT ACT_SITE 161
FT /note="Proton donor"
FT /evidence="ECO:0000256|PIRSR:PIRSR001084-1"
FT ACT_SITE 320
FT /note="Nucleophile"
FT /evidence="ECO:0000256|PIRSR:PIRSR001084-1"
FT BINDING 122
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR001084-2"
FT BINDING 160
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR001084-2"
FT BINDING 328
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR001084-2"
SQ SEQUENCE 695 AA; 77475 MW; F26B790038AEED97 CRC64;
MNIRRPFRWP NLLTETGRGI AYGGDYNPDQ WPEEVWDEDI RLMTKAGVNT VALAIFSWDR
IQPAEDTWDF DWLDRIIGKL GKAGIAVDLA SATAAAPLWL YRAHPEVLPV EANGNVVHAG
SRQSWRPTSP VFREYALTLC RKLAERYKDN PYVTAWHMGN EYGWNNAVDY SDDAVRAFQR
WCEERYGTPE AVNEAWGAAF WSQEVRSFDE IDVPRHMGAD SMVNPAKQLD YTRFCSDALK
EFYRTERDAI EEICPDKPFT TNFMVSTDQC TLDYADWSAE VDFVSNDHYF VPGESHLDEL
ACSDSLVSGF AGHNPWYLME HSTSAVQWKP VNTRKRNGEM VRDALAHVAM GADAVNFFQW
RQSRSGAEAF HSAMVPHAGP DTTVFREVCD LGVILKRLSD SGVQGTCPVD APTAILFSAG
AEWATRSRTL PSGQLSHWHD IRDWYRAFLD TGSRADVVPL SADWSGYGTV ILPTVLVLSD
EDTARLAAFA EDGGRVVVDY ATGIVDDNFR TGLGGYPGAG DGLLRRMLGV RGEEFNILGP
IDGEPDAVRL SNGAVSRLWQ TVVTSVAADT EIVASYHGAD ASEWQIDGVP AVTRHPYGKG
EAWYVGCDLD RADIAHLLRF ELARSHACTD PTHRHDPRIL HVARVGEGGR FDWYFNRAKE
PVEVRVGGEV VVLFRGDDVP GGYVLQRNGV VLVRS
//
