ID A0A087ECU9_9BIFI Unreviewed; 503 AA.
AC A0A087ECU9;
DT 29-OCT-2014, integrated into UniProtKB/TrEMBL.
DT 29-OCT-2014, sequence version 1.
DT 24-JAN-2024, entry version 35.
DE SubName: Full=Sucrose phosphorylase {ECO:0000313|EMBL:MSS12523.1};
DE EC=2.4.1.7 {ECO:0000313|EMBL:KFJ05600.1, ECO:0000313|EMBL:MSS12523.1};
DE SubName: Full=Trey {ECO:0000313|EMBL:KFJ05600.1};
GN ORFNames=BITS_0287 {ECO:0000313|EMBL:KFJ05600.1}, FYJ32_03535
GN {ECO:0000313|EMBL:MSS12523.1};
OS Bifidobacterium tsurumiense.
OC Bacteria; Actinomycetota; Actinomycetes; Bifidobacteriales;
OC Bifidobacteriaceae; Bifidobacterium.
OX NCBI_TaxID=356829 {ECO:0000313|EMBL:KFJ05600.1, ECO:0000313|Proteomes:UP000029080};
RN [1] {ECO:0000313|EMBL:KFJ05600.1, ECO:0000313|Proteomes:UP000029080}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=JCM 13495 {ECO:0000313|EMBL:KFJ05600.1,
RC ECO:0000313|Proteomes:UP000029080};
RA Ventura M., Milani C., Lugli G.A.;
RT "Genomics of Bifidobacteria.";
RL Submitted (MAR-2014) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|EMBL:MSS12523.1, ECO:0000313|Proteomes:UP000482439}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=BSM-380-WT-2B {ECO:0000313|EMBL:MSS12523.1,
RC ECO:0000313|Proteomes:UP000482439};
RA Wylensek D., Hitch T.C.A., Clavel T.;
RT "In-depth cultivation of the pig gut microbiome towards novel bacterial
RT diversity and tailored functional studies.";
RL Submitted (AUG-2019) to the EMBL/GenBank/DDBJ databases.
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 13 family. Sucrose
CC phosphorylase subfamily. {ECO:0000256|ARBA:ARBA00008452}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KFJ05600.1}.
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DR EMBL; JGZU01000015; KFJ05600.1; -; Genomic_DNA.
DR EMBL; VULW01000001; MSS12523.1; -; Genomic_DNA.
DR RefSeq; WP_026641718.1; NZ_VULW01000001.1.
DR AlphaFoldDB; A0A087ECU9; -.
DR STRING; 356829.BITS_0287; -.
DR eggNOG; COG0366; Bacteria.
DR OrthoDB; 9805159at2; -.
DR Proteomes; UP000029080; Unassembled WGS sequence.
DR Proteomes; UP000482439; Unassembled WGS sequence.
DR GO; GO:0009018; F:sucrose phosphorylase activity; IEA:UniProtKB-EC.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR CDD; cd11355; AmyAc_Sucrose_phosphorylase; 1.
DR Gene3D; 2.20.220.10; alpha-Amylases; 1.
DR Gene3D; 3.20.20.80; Glycosidases; 1.
DR InterPro; IPR006047; Glyco_hydro_13_cat_dom.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR InterPro; IPR045857; O16G_dom_2.
DR InterPro; IPR015325; Suc_Porlyase_C.
DR InterPro; IPR016377; Sucrose_GGa_phosphorylase-rel.
DR InterPro; IPR022527; Sucrose_phospho.
DR NCBIfam; TIGR03852; sucrose_gtfA; 1.
DR PANTHER; PTHR38784; SUCROSE PHOSPHORYLASE; 1.
DR PANTHER; PTHR38784:SF1; SUCROSE PHOSPHORYLASE; 1.
DR Pfam; PF00128; Alpha-amylase; 1.
DR Pfam; PF09244; Suc_Porlyase_C; 1.
DR PIRSF; PIRSF003059; Sucrose_phosphorylase; 1.
DR SMART; SM00642; Aamy; 1.
DR SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR SUPFAM; SSF51011; Glycosyl hydrolase domain; 1.
PE 3: Inferred from homology;
KW Glycosyltransferase {ECO:0000256|ARBA:ARBA00022676,
KW ECO:0000313|EMBL:KFJ05600.1};
KW Reference proteome {ECO:0000313|Proteomes:UP000029080};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000313|EMBL:KFJ05600.1}.
FT DOMAIN 6..430
FT /note="Glycosyl hydrolase family 13 catalytic"
FT /evidence="ECO:0000259|SMART:SM00642"
FT ACT_SITE 192
FT /note="Nucleophile"
FT /evidence="ECO:0000256|PIRSR:PIRSR003059-1"
FT ACT_SITE 232
FT /note="Proton donor"
FT /evidence="ECO:0000256|PIRSR:PIRSR003059-1"
FT BINDING 50
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR003059-2"
FT BINDING 88
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR003059-2"
FT BINDING 190..192
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR003059-2"
FT BINDING 232
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR003059-2"
FT BINDING 289..290
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR003059-2"
FT BINDING 342..345
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR003059-2"
FT BINDING 399
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR003059-2"
SQ SEQUENCE 503 AA; 55909 MW; FF6CDC7919364D72 CRC64;
MKNKVQLITY ADRLGDGTIK SLTDVLRTRF DGVYEGVHIL PFFTPFDGAD AGFDPVDHAQ
VDPRLGGWDD VTELSKTHDI MVDTIVNHMS WESKQFQDVM KNGEASPYYK MFLTMSTIFP
DGATEEDLAG IYRPRPGLPF THYTWAGKTR LVWTTFTPQQ VDIDTDSAEG WGYLTSILDR
LAESHVKFIR LDAVGYGAKE AKTSCFMTPK TFDLIERIKE EGTKRGLETL IEVHSYYKKQ
IEIASKVDRV YDFALPPLLL HSLFTGDVDA VAHWVDVRPN NAVTVLDTHD GIGVIDIGSD
QLDRGLKGLV DDESVDSLVN TIHKNTDGES QAATGAAASN LDLYQVNSTY YSALGCNDQH
YIAARAVQFF VPGVPQVYYV GALAGANDMD LLKRTNVGRD INRHYYSTAE VDENLERPVV
KALNALCKLR NTLDAFDGEF SYRKEGESLV FEWQGDNSSA SLEFEPGRGL GRDNESSVIA
LRWTDAAGEH YTDDVIANPP VAA
//
