ID A0A087SCW7_AUXPR Unreviewed; 2211 AA.
AC A0A087SCW7;
DT 29-OCT-2014, integrated into UniProtKB/TrEMBL.
DT 29-OCT-2014, sequence version 1.
DT 27-MAR-2024, entry version 40.
DE RecName: Full=Beta-amylase {ECO:0000256|ARBA:ARBA00012594, ECO:0000256|RuleBase:RU000509};
DE EC=3.2.1.2 {ECO:0000256|ARBA:ARBA00012594, ECO:0000256|RuleBase:RU000509};
GN ORFNames=F751_2830 {ECO:0000313|EMBL:KFM23571.1};
OS Auxenochlorella protothecoides (Green microalga) (Chlorella
OS protothecoides).
OC Eukaryota; Viridiplantae; Chlorophyta; core chlorophytes; Trebouxiophyceae;
OC Chlorellales; Chlorellaceae; Auxenochlorella.
OX NCBI_TaxID=3075 {ECO:0000313|EMBL:KFM23571.1, ECO:0000313|Proteomes:UP000028924};
RN [1] {ECO:0000313|EMBL:KFM23571.1, ECO:0000313|Proteomes:UP000028924}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=0710 {ECO:0000313|EMBL:KFM23571.1,
RC ECO:0000313|Proteomes:UP000028924};
RX PubMed=25012212; DOI=10.1186/1471-2164-15-582;
RA Gao C., Wang Y., Shen Y., Yan D., He X., Dai J., Wu Q.;
RT "Oil accumulation mechanisms of the oleaginous microalga Chlorella
RT protothecoides revealed through its genome, transcriptomes, and
RT proteomes.";
RL BMC Genomics 15:582-582(2014).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Hydrolysis of (1->4)-alpha-D-glucosidic linkages in
CC polysaccharides so as to remove successive maltose units from the
CC non-reducing ends of the chains.; EC=3.2.1.2;
CC Evidence={ECO:0000256|ARBA:ARBA00000546,
CC ECO:0000256|RuleBase:RU000509};
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 14 family.
CC {ECO:0000256|ARBA:ARBA00005652, ECO:0000256|RuleBase:RU000509}.
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DR EMBL; KL662095; KFM23571.1; -; Genomic_DNA.
DR RefSeq; XP_011396445.1; XM_011398143.1.
DR GeneID; 23614221; -.
DR KEGG; apro:F751_2830; -.
DR eggNOG; KOG1064; Eukaryota.
DR OrthoDB; 5480252at2759; -.
DR Proteomes; UP000028924; Unassembled WGS sequence.
DR GO; GO:0102229; F:amylopectin maltohydrolase activity; IEA:UniProtKB-EC.
DR GO; GO:0016161; F:beta-amylase activity; IEA:UniProtKB-EC.
DR GO; GO:0000272; P:polysaccharide catabolic process; IEA:UniProtKB-KW.
DR Gene3D; 3.20.20.80; Glycosidases; 2.
DR Gene3D; 2.130.10.10; YVTN repeat-like/Quinoprotein amine dehydrogenase; 1.
DR InterPro; IPR001554; Glyco_hydro_14.
DR InterPro; IPR018238; Glyco_hydro_14_CS.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR InterPro; IPR022033; Rav1p_C.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR InterPro; IPR036322; WD40_repeat_dom_sf.
DR PANTHER; PTHR31352:SF61; BETA-AMYLASE; 1.
DR PANTHER; PTHR31352; BETA-AMYLASE 1, CHLOROPLASTIC; 1.
DR Pfam; PF01373; Glyco_hydro_14; 2.
DR Pfam; PF12234; Rav1p_C; 1.
DR PRINTS; PR00750; BETAAMYLASE.
DR SUPFAM; SSF51445; (Trans)glycosidases; 2.
DR SUPFAM; SSF50978; WD40 repeat-like; 1.
DR PROSITE; PS00506; BETA_AMYLASE_1; 1.
PE 3: Inferred from homology;
KW Carbohydrate metabolism {ECO:0000256|ARBA:ARBA00023277,
KW ECO:0000256|RuleBase:RU000509};
KW Glycosidase {ECO:0000256|ARBA:ARBA00023295, ECO:0000256|RuleBase:RU000509};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|RuleBase:RU000509};
KW Polysaccharide degradation {ECO:0000256|ARBA:ARBA00023326,
KW ECO:0000256|RuleBase:RU000509};
KW Reference proteome {ECO:0000313|Proteomes:UP000028924}.
FT DOMAIN 1395..1527
FT /note="RAVE complex protein Rav1 C-terminal"
FT /evidence="ECO:0000259|Pfam:PF12234"
FT REGION 1..22
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 256..276
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 321..361
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 906..926
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1369..1390
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1802..1822
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 321..345
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 218
FT /note="Proton donor"
FT /evidence="ECO:0000256|PIRSR:PIRSR601554-1"
FT ACT_SITE 473
FT /note="Proton acceptor"
FT /evidence="ECO:0000256|PIRSR:PIRSR601554-1"
SQ SEQUENCE 2211 AA; 228750 MW; 65EB182742E2F45F CRC64;
MVPQAAWEAG QPGSHSNFQS ARVSMGEEEP ASCATTSVPV MVMLPLDTVN ADGVFRYASS
KWFLPAIRQL AATGIHGVAV DVWWGAVERS PRRYDWSGYR QLFSAIKPLG LKFQVVMSFH
ACGGNVGDNA LVPLPPWVHK VGDSDPDIFF TDRPRESSPG QRNREYISWF AETEPGLLRG
RSVMECYIDF MRAFRDEFMG ELGSLIEEVV VGSGPCGELR YPAYPETNGW RFPGIGEFQC
YDRRALASLA RAAGAAGHPE WGNSGPSNAG SYNSHPEETG FFQTWNGGWQ SPQGEFFLGW
YSDALLEHGE RMLTAVTSVF NSPGGSSSGD PSPRSSDTMV GSPHTSVGVG GTPPPANGTP
DGMMAAVSFA GMASPGGAGG FNGPFGSASL LPPAARPDQD HAAELTAGYY NTPERDGYAA
LCALCARHGA AATLTCVEMC DAQHPPEALC GPEGVLRQVR EAAAAHGVPL AGENALPCFM
PASVDEAALA RILPPLRGCT FLRLTRELMA PSYQEHWVRF MAQMKVNESA RPSGDSVPLH
TLLVTPVAAS PSDPVVLVRW GGGPTALVAA ATASELVVVS LAGTLGIGPW ESHAGTRTGK
VVARRPLPSP LSGCSWTARV DGLFVCLADG TLSMLRLDWP ARRLTEHWTI RPPEAQGTLA
AGQSPAAPAA SAAAPACRDP AVSVWWPPAE SGAQGSGARR EKLRHPCPVL GLAWSRRLDS
FFCMAMVIQG PAQGFYPGSL RAAWAEEEPE GPPGMEPAGT APHISTLTRV HVVRALQAVV
VSSWRAEEGP SSAGPGAVTS AQAVLWGQLE QRRPRGAHHM DSPPSLTCRL EAREGSSTIL
VRIDCSRFAT VPAAVQEPGT FFRPRPTPRT AGLPPALTLL SAWRGAATGH ATPVIDIAGS
PYGGQLGSLD SGGPASDGDT RSRPLRRRWR TQDAALALAT TGDLVVVGGT GDALVEALPP
GTRAVALHAI AVAGGDRGAA LLLLETAAQD RAGPRLAWTL RWGKERSLTG CAEGAWGGAE
HVRVLGAGLD LLLSAERGAL RAWRAVERDG ESGAELVPAG LYALPDEATV LVAAAGACGL
VAALTGGPAL ADSTPLADAS AGKKAADPPT LLLHVWRGRH AAVQTLPPLA RDGVTTAASL
AWVPHAVAPA LAVVLGADAL LYCQQPGGRT IAARHGPLPA HGATTLAALL ACGAPGVAVH
LLIARAAALE GGAPGGEAEA ALQRALAALW AAWSVALRLP ALLAGLREAT ATHVHGWERE
RNDPDLPDPE RVTAAICRLG SNGGGALARV LAASDSAGHA RLDPAGRAFL DLARAAGHHG
MPQGGLAASL QLHSGLGLEA VVWALMSGTQ EVLLEEALPS LAAAVDGSTA ASESGPSAAP
SFLGTRADAG HAQPPTWEAL RAAGAGFWLR DPGLVLDVGE KLAKAQFAAA RKPDACALLY
VALGKKATLQ GLYRTTNARK QADFLGRDFG LPANAQSAAK NAFVLLGQHR FSLAAAFFLL
GGHVSDALDV CVRELSDLQL ALFIRRIVVL VMGGKGGGVD HASGPNAPAA PHHLEDWGWG
AAARAALLGD DAGLLCALCW RVRWERGGAL AAAQLPALAA ALLPHIVGVE AAVYDVKRER
DGDAIDDADH ATLLAQQLRG ALTAAATSLD AARLPLLALG SAAAAIAIGP AAKEGEETGL
SKTLDPLTPF LARLAASALE MAFAPEALPE RLGSERDSSC STLGLGALSR VSSSSRGGGE
GTAAAAWELK RQPIVCVDGD RCGALALCSL TSPDELGGRA LVVSTHRHGL VASDVIPLES
AAHRGAAEAG SRPETPVSTP DVGGFQAPAA QGGIFSRIIS HIFDQASWEE SEKWGSSTMA
ESDLGFGGFV GGPTSPHTPP ARGHIDGLPE LGSMHASALC AHPTRQFFLS ADGHGFVHLW
QFGEREALAS FTPLPPPDLA SWGSGSLLSM GSRLFSGSLT SQLGGWGCCQ DLAWSPAADA
FAAVGEGGVV ATWRLGRPKG VDADGHACAE WWHQCLARKG RAVTYIDEAV LAVGGEDASG
NLVLWDSRAC SAVARLHPTT PGDGVTRLLG PSSGLWSLAA GTVGGAVVAL DLRRLSGAAA
QPAAVGAGAP PALLWQAAHK KAGAVTALTR LAGGWVAAGY ADGDLRMFDP GLPSAPLVLR
EERAHGARSR RRAAVSGLAG CPEGLVSAGA DGAVFLWTLE RSPEGQSSDY N
//
