ID A0A090AFH1_9GAMM Unreviewed; 510 AA.
AC A0A090AFH1;
DT 26-NOV-2014, integrated into UniProtKB/TrEMBL.
DT 26-NOV-2014, sequence version 1.
DT 27-MAR-2024, entry version 43.
DE RecName: Full=Nif-specific regulatory protein {ECO:0000256|ARBA:ARBA00015308, ECO:0000256|RuleBase:RU368029};
GN ORFNames=THII_2556 {ECO:0000313|EMBL:BAP56853.1};
OS Thioploca ingrica.
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Thiotrichales;
OC Thiotrichaceae; Thioploca.
OX NCBI_TaxID=40754 {ECO:0000313|EMBL:BAP56853.1, ECO:0000313|Proteomes:UP000031623};
RN [1] {ECO:0000313|EMBL:BAP56853.1, ECO:0000313|Proteomes:UP000031623}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Kojima H., Ogura Y., Yamamoto N., Togashi T., Mori H., Watanabe T.,
RA Nemoto F., Kurokawa K., Hayashi T., Fukui M.;
RT "Ecophysiology of Thioploca ingrica as revealed by the complete genome
RT sequence supplemented with proteomic evidence.";
RL ISME J. 0:0-0(2014).
CC -!- FUNCTION: Required for activation of most nif operons, which are
CC directly involved in nitrogen fixation. {ECO:0000256|ARBA:ARBA00002167,
CC ECO:0000256|RuleBase:RU368029}.
CC -!- SUBUNIT: Interacts with sigma-54. {ECO:0000256|ARBA:ARBA00011135,
CC ECO:0000256|RuleBase:RU368029}.
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DR EMBL; AP014633; BAP56853.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A090AFH1; -.
DR STRING; 40754.THII_2556; -.
DR KEGG; tig:THII_2556; -.
DR HOGENOM; CLU_000445_95_2_6; -.
DR OrthoDB; 5297379at2; -.
DR Proteomes; UP000031623; Chromosome.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:InterPro.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IEA:UniProtKB-UniRule.
DR GO; GO:0043565; F:sequence-specific DNA binding; IEA:InterPro.
DR GO; GO:0009399; P:nitrogen fixation; IEA:UniProtKB-UniRule.
DR GO; GO:0000160; P:phosphorelay signal transduction system; IEA:UniProtKB-UniRule.
DR CDD; cd00009; AAA; 1.
DR Gene3D; 1.10.8.60; -; 1.
DR Gene3D; 3.30.450.40; -; 1.
DR Gene3D; 1.10.10.60; Homeodomain-like; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 1.
DR InterPro; IPR003593; AAA+_ATPase.
DR InterPro; IPR003018; GAF.
DR InterPro; IPR029016; GAF-like_dom_sf.
DR InterPro; IPR002197; HTH_Fis.
DR InterPro; IPR010113; Nif-specific_regulatory_prot.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR002078; Sigma_54_int.
DR InterPro; IPR025662; Sigma_54_int_dom_ATP-bd_1.
DR InterPro; IPR025943; Sigma_54_int_dom_ATP-bd_2.
DR InterPro; IPR025944; Sigma_54_int_dom_CS.
DR NCBIfam; TIGR01817; nifA; 1.
DR PANTHER; PTHR32071; TRANSCRIPTIONAL REGULATORY PROTEIN; 1.
DR PANTHER; PTHR32071:SF95; TRANSCRIPTIONAL REGULATORY PROTEIN ZRAR; 1.
DR Pfam; PF01590; GAF; 1.
DR Pfam; PF02954; HTH_8; 1.
DR Pfam; PF00158; Sigma54_activat; 1.
DR PRINTS; PR01590; HTHFIS.
DR SMART; SM00382; AAA; 1.
DR SMART; SM00065; GAF; 1.
DR SUPFAM; SSF55781; GAF domain-like; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR PROSITE; PS00675; SIGMA54_INTERACT_1; 1.
DR PROSITE; PS00676; SIGMA54_INTERACT_2; 1.
DR PROSITE; PS00688; SIGMA54_INTERACT_3; 1.
DR PROSITE; PS50045; SIGMA54_INTERACT_4; 1.
PE 4: Predicted;
KW Activator {ECO:0000256|RuleBase:RU368029};
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840};
KW Coiled coil {ECO:0000256|SAM:Coils};
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125, ECO:0000256|RuleBase:RU368029};
KW Nitrogen fixation {ECO:0000256|ARBA:ARBA00023231,
KW ECO:0000256|RuleBase:RU368029};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW Reference proteome {ECO:0000313|Proteomes:UP000031623};
KW Transcription {ECO:0000256|RuleBase:RU368029};
KW Transcription regulation {ECO:0000256|RuleBase:RU368029};
KW Two-component regulatory system {ECO:0000256|RuleBase:RU368029}.
FT DOMAIN 199..427
FT /note="Sigma-54 factor interaction"
FT /evidence="ECO:0000259|PROSITE:PS50045"
FT COILED 158..189
FT /evidence="ECO:0000256|SAM:Coils"
SQ SEQUENCE 510 AA; 57768 MW; AC7F564C7F50BC15 CRC64;
MTVTNRSLLI ETQLAALFRV GQILNHSLNL QETLQGVLQV LHDHTCMQHG MVTLRDEAGT
FSISAIYAGN KSIPLTVRYR PGEGIIGKIL QEGQSLVIER IADEPRFLDR LGLYDPQLPF
IGVPIHVIEE DKPIGVLIAQ PNTNDGLLTE RIRFMEMVAQ LIAQSVRLAK EIERERRDLT
DERDRLRRAV RGNYGFDNII GHTSAMQRVF ELVRQVAKWN TTVLIRGESG TGKELIANAI
HYNSPRHHNE FIKVNCAAIP ESLLESELFG HEKGAFSGAI NQHKGRFERA NKGTLFLDEI
GDISPTFQAK LLRVLQEGEF ERVGGSRTLK VDVRIITATH VDLESRVEQG EFREDLYYRL
NVMPISLPAL RERPEDIPEL AQFLLDKVAK KQGRHLELTD SAIRLLMQHS WPGNVRELEN
CLERAAILGE GERLDRAVIN LAGLKEEIPL PRNPPAKVDL QDPKLDERER VIAALEQAGW
VQAKAARLLD MTPRQIAYRI QTLNIKMRNI
//
