ID A0A093FC91_GAVST Unreviewed; 595 AA.
AC A0A093FC91;
DT 26-NOV-2014, integrated into UniProtKB/TrEMBL.
DT 26-NOV-2014, sequence version 1.
DT 27-MAR-2024, entry version 38.
DE RecName: Full=Calpain-2 catalytic subunit {ECO:0000256|ARBA:ARBA00014052};
DE EC=3.4.22.53 {ECO:0000256|ARBA:ARBA00012481};
DE AltName: Full=Calcium-activated neutral proteinase 2 {ECO:0000256|ARBA:ARBA00031308};
DE AltName: Full=Calpain M-type {ECO:0000256|ARBA:ARBA00032449};
DE AltName: Full=Calpain-2 large subunit {ECO:0000256|ARBA:ARBA00032544};
DE AltName: Full=Millimolar-calpain {ECO:0000256|ARBA:ARBA00030109};
DE Flags: Fragment;
GN ORFNames=N328_02485 {ECO:0000313|EMBL:KFV51854.1};
OS Gavia stellata (Red-throated diver) (Colymbus stellatus).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Archelosauria; Archosauria; Dinosauria; Saurischia; Theropoda;
OC Coelurosauria; Aves; Neognathae; Gaviiformes; Gaviidae; Gavia.
OX NCBI_TaxID=37040 {ECO:0000313|EMBL:KFV51854.1, ECO:0000313|Proteomes:UP000054313};
RN [1] {ECO:0000313|EMBL:KFV51854.1, ECO:0000313|Proteomes:UP000054313}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=BGI_N328 {ECO:0000313|EMBL:KFV51854.1};
RA Zhang G., Li C.;
RT "Genome evolution of avian class.";
RL Submitted (APR-2014) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Broad endopeptidase specificity.; EC=3.4.22.53;
CC Evidence={ECO:0000256|ARBA:ARBA00001223};
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000256|ARBA:ARBA00004236}.
CC Membrane {ECO:0000256|ARBA:ARBA00004370}.
CC -!- SIMILARITY: Belongs to the peptidase C2 family.
CC {ECO:0000256|ARBA:ARBA00007623}.
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DR EMBL; KK622293; KFV51854.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A093FC91; -.
DR Proteomes; UP000054313; Unassembled WGS sequence.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005509; F:calcium ion binding; IEA:InterPro.
DR GO; GO:0004198; F:calcium-dependent cysteine-type endopeptidase activity; IEA:InterPro.
DR GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR CDD; cd00214; Calpain_III; 1.
DR CDD; cd00044; CysPc; 1.
DR Gene3D; 2.60.120.380; -; 1.
DR Gene3D; 3.90.70.10; Cysteine proteinases; 1.
DR Gene3D; 1.10.238.10; EF-hand; 1.
DR InterPro; IPR033883; C2_III.
DR InterPro; IPR022684; Calpain_cysteine_protease.
DR InterPro; IPR022682; Calpain_domain_III.
DR InterPro; IPR022683; Calpain_III.
DR InterPro; IPR036213; Calpain_III_sf.
DR InterPro; IPR011992; EF-hand-dom_pair.
DR InterPro; IPR018247; EF_Hand_1_Ca_BS.
DR InterPro; IPR002048; EF_hand_dom.
DR InterPro; IPR038765; Papain-like_cys_pep_sf.
DR InterPro; IPR001300; Peptidase_C2_calpain_cat.
DR PANTHER; PTHR10183; CALPAIN; 1.
DR PANTHER; PTHR10183:SF268; CALPAIN-2 CATALYTIC SUBUNIT; 1.
DR Pfam; PF01067; Calpain_III; 1.
DR Pfam; PF00648; Peptidase_C2; 1.
DR PRINTS; PR00704; CALPAIN.
DR SMART; SM00720; calpain_III; 1.
DR SMART; SM00230; CysPc; 1.
DR SUPFAM; SSF49758; Calpain large subunit, middle domain (domain III); 1.
DR SUPFAM; SSF54001; Cysteine proteinases; 1.
DR SUPFAM; SSF47473; EF-hand; 1.
DR PROSITE; PS50203; CALPAIN_CAT; 1.
DR PROSITE; PS00018; EF_HAND_1; 1.
DR PROSITE; PS50222; EF_HAND_2; 1.
PE 3: Inferred from homology;
KW Calcium {ECO:0000256|ARBA:ARBA00022837};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|PROSITE-
KW ProRule:PRU00239}; Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Protease {ECO:0000256|ARBA:ARBA00022670, ECO:0000256|PROSITE-
KW ProRule:PRU00239}; Reference proteome {ECO:0000313|Proteomes:UP000054313};
KW Signal {ECO:0000256|SAM:SignalP};
KW Thiol protease {ECO:0000256|ARBA:ARBA00022807, ECO:0000256|PROSITE-
KW ProRule:PRU00239}.
FT SIGNAL 1..20
FT /evidence="ECO:0000256|SAM:SignalP"
FT CHAIN 21..595
FT /note="Calpain-2 catalytic subunit"
FT /evidence="ECO:0000256|SAM:SignalP"
FT /id="PRO_5001885851"
FT DOMAIN 1..242
FT /note="Calpain catalytic"
FT /evidence="ECO:0000259|PROSITE:PS50203"
FT DOMAIN 506..541
FT /note="EF-hand"
FT /evidence="ECO:0000259|PROSITE:PS50222"
FT ACT_SITE 3
FT /evidence="ECO:0000256|PIRSR:PIRSR622684-1,
FT ECO:0000256|PROSITE-ProRule:PRU00239"
FT ACT_SITE 160
FT /evidence="ECO:0000256|PIRSR:PIRSR622684-1,
FT ECO:0000256|PROSITE-ProRule:PRU00239"
FT ACT_SITE 184
FT /evidence="ECO:0000256|PIRSR:PIRSR622684-1,
FT ECO:0000256|PROSITE-ProRule:PRU00239"
FT NON_TER 1
FT /evidence="ECO:0000313|EMBL:KFV51854.1"
FT NON_TER 595
FT /evidence="ECO:0000313|EMBL:KFV51854.1"
SQ SEQUENCE 595 AA; 68470 MW; FF9C1B73DA8323D6 CRC64;
GDCWLLAAIA SLTLNEEILA RVVPKDQSFQ DKYAGIFHFQ FWQYGEWVDV VVDDRLPTKN
GELLFVHSAE GSEFWSALLE KAYAKLNGSY ESLSGGTTTE GFEDFTGGIA EWYELQKAPP
NLFKIIQKAL QKGSLLGCSI DITSAAETEA VTSQKLVKGH AYSVTGAEEV NFRGTVQKLI
RIRNPWGEVE WTGKWNDNCP NWSGVDPEVR EQLTRRHEDG EFWMAFNDFL RHYSRLEICN
LTPDTLASDR YKKWSLLKLD GNWRRGATAG GCRNYPNTFW TNPQYLIKLE EEDEDPDDPE
RGCTFLIGLI QKHRRKQRKM GEDMHTIGFA IYEASKAVQF SGQTNIHLSK NFFLTNKARE
KSNTFINLRE CLRSIRRQDA PTAAGGWRET LLFPLWHVRM TLKQQKVRAL KGKKEKEKSD
WITDITEDDI EPSFKKLFGQ LAGSDAEISA FELRNILNKI MAKRQDIKSD GFSIETCKIM
VDLSLNSNKN DGSGKLGLKE FHTLWTKIQK YQKIYREIDV DRSGTMNSYE MRRALETAGF
KLNCQLHQII VARFADEDLI IDFDNFVRCL IRLETLFKMF RKLDTEKTGT IELNL
//
