ID A0A093Y8Z0_9PEZI Unreviewed; 1539 AA.
AC A0A093Y8Z0;
DT 26-NOV-2014, integrated into UniProtKB/TrEMBL.
DT 26-NOV-2014, sequence version 1.
DT 24-JAN-2024, entry version 35.
DE RecName: Full=Glycogen debranching enzyme {ECO:0000256|ARBA:ARBA00020723};
DE EC=2.4.1.25 {ECO:0000256|ARBA:ARBA00012560};
DE EC=3.2.1.33 {ECO:0000256|ARBA:ARBA00012778};
DE AltName: Full=Glycogen debrancher {ECO:0000256|ARBA:ARBA00031477};
GN ORFNames=V490_03879 {ECO:0000313|EMBL:KFX95409.1};
OS Pseudogymnoascus sp. VKM F-3557.
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Leotiomycetes;
OC Leotiomycetes incertae sedis; Pseudeurotiaceae; Pseudogymnoascus.
OX NCBI_TaxID=1437433 {ECO:0000313|EMBL:KFX95409.1, ECO:0000313|Proteomes:UP000029320};
RN [1] {ECO:0000313|EMBL:KFX95409.1, ECO:0000313|Proteomes:UP000029320}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=VKM F-3557 {ECO:0000313|EMBL:KFX95409.1,
RC ECO:0000313|Proteomes:UP000029320};
RA Leushkin E.V., Logacheva M.D., Penin A.A., Sutormin R.A., Gerasimov E.S.,
RA Kochkina G.A., Ivanushkina N.E., Vasilenko O.V., Kondrashov A.S.,
RA Ozerskaya S.M.;
RT "Population genomics of a fungus Geomyces pannorum provides evidence of
RT horizontal gene transfer but not of sexual reproduction.";
RL Submitted (JUL-2014) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Multifunctional enzyme acting as 1,4-alpha-D-glucan:1,4-
CC alpha-D-glucan 4-alpha-D-glycosyltransferase and amylo-1,6-glucosidase
CC in glycogen degradation. {ECO:0000256|ARBA:ARBA00003530}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Hydrolysis of (1->6)-alpha-D-glucosidic branch linkages in
CC glycogen phosphorylase limit dextrin.; EC=3.2.1.33;
CC Evidence={ECO:0000256|ARBA:ARBA00000927};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Transfers a segment of a (1->4)-alpha-D-glucan to a new
CC position in an acceptor, which may be glucose or a (1->4)-alpha-D-
CC glucan.; EC=2.4.1.25; Evidence={ECO:0000256|ARBA:ARBA00000439};
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|ARBA:ARBA00004496}.
CC -!- SIMILARITY: Belongs to the glycogen debranching enzyme family.
CC {ECO:0000256|ARBA:ARBA00025780}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KFX95409.1}.
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DR EMBL; JPJS01001035; KFX95409.1; -; Genomic_DNA.
DR STRING; 1437433.A0A093Y8Z0; -.
DR HOGENOM; CLU_001517_2_1_1; -.
DR Proteomes; UP000029320; Unassembled WGS sequence.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0004134; F:4-alpha-glucanotransferase activity; IEA:UniProtKB-EC.
DR GO; GO:0004135; F:amylo-alpha-1,6-glucosidase activity; IEA:UniProtKB-EC.
DR GO; GO:0102500; F:beta-maltose 4-alpha-glucanotransferase activity; IEA:UniProtKB-EC.
DR GO; GO:0005978; P:glycogen biosynthetic process; IEA:UniProtKB-KW.
DR GO; GO:0005980; P:glycogen catabolic process; IEA:InterPro.
DR CDD; cd11327; AmyAc_Glg_debranch_2; 1.
DR Gene3D; 1.50.10.10; -; 1.
DR Gene3D; 3.20.20.80; Glycosidases; 2.
DR InterPro; IPR008928; 6-hairpin_glycosidase_sf.
DR InterPro; IPR012341; 6hp_glycosidase-like_sf.
DR InterPro; IPR010401; AGL/Gdb1.
DR InterPro; IPR032788; AGL_central.
DR InterPro; IPR029436; AGL_euk_N.
DR InterPro; IPR032792; AGL_glucanoTrfase.
DR InterPro; IPR032790; GDE_C.
DR InterPro; IPR006421; Glycogen_debranch_met.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR NCBIfam; TIGR01531; glyc_debranch; 1.
DR PANTHER; PTHR10569; GLYCOGEN DEBRANCHING ENZYME; 1.
DR PANTHER; PTHR10569:SF2; GLYCOGEN DEBRANCHING ENZYME; 1.
DR Pfam; PF06202; GDE_C; 1.
DR Pfam; PF14701; hDGE_amylase; 1.
DR Pfam; PF14702; hGDE_central; 1.
DR Pfam; PF14699; hGDE_N; 1.
DR SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR SUPFAM; SSF48208; Six-hairpin glycosidases; 1.
PE 3: Inferred from homology;
KW Cytoplasm {ECO:0000256|ARBA:ARBA00022490};
KW Glycogen biosynthesis {ECO:0000256|ARBA:ARBA00023056};
KW Glycosidase {ECO:0000256|ARBA:ARBA00023295};
KW Glycosyltransferase {ECO:0000256|ARBA:ARBA00022676};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW Multifunctional enzyme {ECO:0000256|ARBA:ARBA00023268};
KW Reference proteome {ECO:0000313|Proteomes:UP000029320};
KW Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT DOMAIN 42..137
FT /note="Eukaryotic glycogen debranching enzyme N-terminal"
FT /evidence="ECO:0000259|Pfam:PF14699"
FT DOMAIN 140..577
FT /note="Glycogen debranching enzyme glucanotransferase"
FT /evidence="ECO:0000259|Pfam:PF14701"
FT DOMAIN 732..976
FT /note="Glycogen debranching enzyme central"
FT /evidence="ECO:0000259|Pfam:PF14702"
FT DOMAIN 1054..1522
FT /note="Glycogen debranching enzyme C-terminal"
FT /evidence="ECO:0000259|Pfam:PF06202"
FT REGION 1410..1437
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1419..1434
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1539 AA; 172702 MW; D884899C9C3CC8FC CRC64;
MAPNTSNAAK EVYLLPLLDD GSPDVPGGYI YLEPTSAEPI IVRFAIEGTS SICRHGSLWV
NINAPGEPFK RDAFREFKLE PDFNRTLEID IPIHSSGAFA FYTTYSPLPD LDDAGSKPSE
PTKTPLYYID VAPRLNLGEK QLPMAALSLI SVLSKFMGKY PTDWQKHLRG ISERGYNMIH
FTPLQVRGHS DSPYSIADQL GWDPECFPGG EKDIAQLVNV MEKEHGLLGL TDVVWNHTAD
NSTWLQEHPE VGYNMSTAPW LESALQLDTE LLKFGRELEK LGLPTDIKTV EDLLLIMDGI
KTKVIGGLKL WEYYAIDVKR DVDAAVDAWV SGNVKAPAEG FGSKDASPKE HAKFIVDKAM
LGTDRLGERY RRKVDPAIAA ALLTEWHGKF NGNASNPADS EEVRSHTSKI FDEVNLPFYK
EYDEDIAEVL EQIFNRVKYV RLDDHGPKLG PINDKNPLIE SYFTRLPQNA TTKKHKQEDL
ALVNNGWIWA ANALVDNAGP NSRAYLRREV IVWGDCVKLK YGDGPQDSPY LWERMANYTR
LMAKYFTGLR IDNAHSTPLH VAEYLLDEAR RVRPDLFVVA ELFTGSEEMD YVFVKRLGIN
GLIREAMQAW NTGELSRLVH RHGGRPIGSF EVDEISGNDA KESTEIVRKV KQTPVRALFM
DCTHDNEVPA QKRDARDTLP NAALVYMCAS ATGSVFGYDE IYPKIIDLVH ETRLYTSHSS
EKPVDIKDGE GGIGGVRKLL NRIHILMGLD GYEETHIHHD DQYVTVHRVH PESRKGYFLI
AHTAFPGYKN GNGPFSPVHL TGTKANHLGS WMLEVDDSEE ARKEASEDKQ YLKGLPSTVT
SVPGIKMESK DDETVITMGD KFPPGSIALF ETWIPAAEHA SGLDTFVTLG AKEAFSKVDL
VDLNFIMYRC EGEELDSSDG KDGVYDIPSH GKLVYAGLEG WWSVLKKVIN GNDLAHPLAQ
HLRSGQWALD YTVGRLQRKS KEEGFERLQG PALWLQERFD AIRKLPSFLL PRYFGLIIKT
AYSAGFDRGV ELMSENVQKG QWFMKSLAMV SVQQTGFVKS ASLYPKRAVP SLAAGLPHFA
VEWARCWGRD VFISARGLLL GTGRYAEARE HIIAFASVVK HGMIPNLLSS GNLPRYNSRD
SVWFFLQTIQ DYTKIVPNGY DLLQEKISRR FLPYDDTYFE SDDPRAYSKT STLEDIIQEI
FQRHASGMSF REANAGPKLD MQMKPEGFQI DIKVDWNTGI IFGGSQDNCG TWMDKMGESE
KAGTRGVPGT PRDGAAVEIT GLSYSALKWV SQLNKDGKFK YSGVNTNDSS AKEISFADWA
SKIRDNFERC YYVPASSNED AKYDVNPAII NRRGIYKDLY KSGKEYEDYQ LRPNFPIAMT
VAPDLFDDKH ALGALFIADK ALRGPTGMAT LDPSDLNYRP DYHNSEDSTD KATSKGRNYH
QGPEWLWPTG FFLRALLAFD LKRRDTPEGR TEAFQQVTRR LADSKKAIVE SEWAGLTELT
NKSGSFCADS SPTQAWSAGC FIDLYHDAAQ YDISQLQEK
//
