ID A0A094DMG4_9PEZI Unreviewed; 1078 AA.
AC A0A094DMG4;
DT 26-NOV-2014, integrated into UniProtKB/TrEMBL.
DT 26-NOV-2014, sequence version 1.
DT 13-SEP-2023, entry version 37.
DE RecName: Full=isoleucine--tRNA ligase {ECO:0000256|ARBA:ARBA00013165};
DE EC=6.1.1.5 {ECO:0000256|ARBA:ARBA00013165};
DE AltName: Full=Isoleucyl-tRNA synthetase {ECO:0000256|ARBA:ARBA00032665};
GN ORFNames=V496_02614 {ECO:0000313|EMBL:KFY65383.1};
OS Pseudogymnoascus sp. VKM F-4515 (FW-2607).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Leotiomycetes;
OC Leotiomycetes incertae sedis; Pseudeurotiaceae; Pseudogymnoascus.
OX NCBI_TaxID=1420909 {ECO:0000313|EMBL:KFY65383.1, ECO:0000313|Proteomes:UP000029302};
RN [1] {ECO:0000313|EMBL:KFY65383.1, ECO:0000313|Proteomes:UP000029302}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=VKM F-4515 (FW-2607) {ECO:0000313|Proteomes:UP000029302};
RA Leushkin E.V., Logacheva M.D., Penin A.A., Sutormin R.A., Gerasimov E.S.,
RA Kochkina G.A., Ivanushkina N.E., Vasilenko O.V., Kondrashov A.S.,
RA Ozerskaya S.M.;
RT "Population genomics of a fungus Geomyces pannorum provides evidence of
RT horizontal gene transfer but not of sexual reproduction.";
RL Submitted (JUL-2014) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-
CC isoleucyl-tRNA(Ile); Xref=Rhea:RHEA:11060, Rhea:RHEA-COMP:9666,
CC Rhea:RHEA-COMP:9695, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019,
CC ChEBI:CHEBI:58045, ChEBI:CHEBI:78442, ChEBI:CHEBI:78528,
CC ChEBI:CHEBI:456215; EC=6.1.1.5;
CC Evidence={ECO:0000256|ARBA:ARBA00000114};
CC -!- SIMILARITY: Belongs to the class-I aminoacyl-tRNA synthetase family.
CC {ECO:0000256|ARBA:ARBA00005594, ECO:0000256|RuleBase:RU363035}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KFY65383.1}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; JPJY01000405; KFY65383.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A094DMG4; -.
DR STRING; 1420909.A0A094DMG4; -.
DR HOGENOM; CLU_001493_1_1_1; -.
DR OrthoDB; 656at2759; -.
DR Proteomes; UP000029302; Unassembled WGS sequence.
DR GO; GO:0002161; F:aminoacyl-tRNA editing activity; IEA:InterPro.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0004822; F:isoleucine-tRNA ligase activity; IEA:UniProtKB-EC.
DR GO; GO:0000049; F:tRNA binding; IEA:InterPro.
DR GO; GO:0006428; P:isoleucyl-tRNA aminoacylation; IEA:InterPro.
DR CDD; cd07961; Anticodon_Ia_Ile_ABEc; 1.
DR CDD; cd00818; IleRS_core; 1.
DR Gene3D; 3.40.50.620; HUPs; 2.
DR HAMAP; MF_02003; Ile_tRNA_synth_type2; 1.
DR InterPro; IPR001412; aa-tRNA-synth_I_CS.
DR InterPro; IPR002300; aa-tRNA-synth_Ia.
DR InterPro; IPR033709; Anticodon_Ile_ABEc.
DR InterPro; IPR002301; Ile-tRNA-ligase.
DR InterPro; IPR023586; Ile-tRNA-ligase_type2.
DR InterPro; IPR013155; M/V/L/I-tRNA-synth_anticd-bd.
DR InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR InterPro; IPR009080; tRNAsynth_Ia_anticodon-bd.
DR InterPro; IPR009008; Val/Leu/Ile-tRNA-synth_edit.
DR NCBIfam; TIGR00392; ileS; 1.
DR PANTHER; PTHR42780:SF1; ISOLEUCINE--TRNA LIGASE, CYTOPLASMIC; 1.
DR PANTHER; PTHR42780; SOLEUCYL-TRNA SYNTHETASE; 1.
DR Pfam; PF08264; Anticodon_1; 1.
DR Pfam; PF19302; DUF5915; 1.
DR Pfam; PF00133; tRNA-synt_1; 1.
DR PRINTS; PR00984; TRNASYNTHILE.
DR SUPFAM; SSF47323; Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases; 1.
DR SUPFAM; SSF52374; Nucleotidylyl transferase; 1.
DR SUPFAM; SSF50677; ValRS/IleRS/LeuRS editing domain; 1.
DR PROSITE; PS00178; AA_TRNA_LIGASE_I; 1.
PE 3: Inferred from homology;
KW Aminoacyl-tRNA synthetase {ECO:0000256|ARBA:ARBA00023146,
KW ECO:0000256|RuleBase:RU363035};
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|RuleBase:RU363035};
KW Ligase {ECO:0000256|ARBA:ARBA00022598, ECO:0000256|RuleBase:RU363035};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741,
KW ECO:0000256|RuleBase:RU363035};
KW Protein biosynthesis {ECO:0000256|ARBA:ARBA00022917,
KW ECO:0000256|RuleBase:RU363035};
KW Reference proteome {ECO:0000313|Proteomes:UP000029302}.
FT DOMAIN 12..634
FT /note="Aminoacyl-tRNA synthetase class Ia"
FT /evidence="ECO:0000259|Pfam:PF00133"
FT DOMAIN 691..845
FT /note="Methionyl/Valyl/Leucyl/Isoleucyl-tRNA synthetase
FT anticodon-binding"
FT /evidence="ECO:0000259|Pfam:PF08264"
SQ SEQUENCE 1078 AA; 123828 MW; 5F7999C3262EDBBC CRC64;
MSVDFPKEEE AVLQRWKEIQ AFERQVELSK GRKPYTFYDG PPFATGLPHY GHLLASTIKD
IIPRYWSMKG YHVERRFGWD THGLPIEHEI DKKLGISGRE AVMKLGLDKY NAECRAIVMR
YSAEWRTTID RLGRWIDFDN DYKTMDPTFM ESIWWVFKQI FDKDAVYQGY RVMPYSTVLT
TALSNFEANQ NYQDIQDPAV VVSFPLLDDP ETSLLAWTTT PWTLPSHTGL CAHPDFEYIK
IRDELSGKNY ILLEKLLGTL YKDPKKAKFK IIEKIKGTQM LGWKYQPLFD YFYEEFKDYG
FRVFNATYVT DDSGVGIVHQ APAFGEEDYN LALEFGVIDK SRGPPNPVDE SGHFTSKVRD
FEGQHVKAAD KAIIKYLKGT GRLIVDSQIK HSYPMCPRSD TPLIYRAVPS WFVKIPEIVP
QMLENIEDSH WVPSFVKEKR FASWIANARD WNVSRNRYWG TPIPLWVSDD LEERVCIGSI
AELKELSGYE GEITDLHRDK IDHITIPSKM GKGTLHRIEE VFDCWFESGS MPYASQHYPF
ENQDKFKASF PGDFIAEGLD QTRGWFYTLL VIGTHLFGKS PFKNCVVNGI VLAEDGKKMS
KRLKNYPDPT IVMSKYGSDA LRLYLINSPV VRAEPLRFKE AGVKEVVAKV LLPLWNSYKF
FEGQVALLKK VEGVDFIFDP ASESTNTNVM DQWILASCQS LLKFVNEEMA AYRLYTVVPR
LLELIDTTTN WYIRFNRKRL KGEFGLDDTI HALNSLFEVL FTLTKGLAPF TPFITENIYG
RLLAHIPKEL RGEDDRSVHF LPFPDVRSEL FNGEVERRVG RMQRVIELAR VSRERRAVGL
KTPLKSLVVI HPDPVYLEDI RSLEGYITEE LNVVSLVLSS DEEKYNVQYS VSADWPVLGK
KLKKDVQRVK KALPALTSAE VQQFVKTKTI TVDGIVLGDG DLHVKRSLKE DDSSKNLETN
TDEDVLVILD AALYPELAEE GLSREIINRV QRLRKKAGLQ PTDDVKMEYN VIADPDNLGL
ADIFRTQAKS IEKALRRPVD QHVVTEVDGV PSTNVDQLIM EEKQEIQKAT FMLRLVKI
//
