ID A0A094PGY1_9ACTN Unreviewed; 728 AA.
AC A0A094PGY1;
DT 26-NOV-2014, integrated into UniProtKB/TrEMBL.
DT 26-NOV-2014, sequence version 1.
DT 27-MAR-2024, entry version 36.
DE RecName: Full=ATP-dependent DNA helicase RecG {ECO:0000256|ARBA:ARBA00017846, ECO:0000256|RuleBase:RU363016};
DE EC=3.6.4.12 {ECO:0000256|ARBA:ARBA00012551, ECO:0000256|RuleBase:RU363016};
GN Name=recG {ECO:0000256|RuleBase:RU363016};
GN ORFNames=GM48_1160 {ECO:0000313|EMBL:KGA10262.1};
OS actinobacterium acIB-AMD-7.
OC Bacteria; Actinomycetota; Actinomycetes.
OX NCBI_TaxID=1504322 {ECO:0000313|EMBL:KGA10262.1, ECO:0000313|Proteomes:UP000029310};
RN [1] {ECO:0000313|EMBL:KGA10262.1, ECO:0000313|Proteomes:UP000029310}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Ghai R., Mizuno C.M., Picazo A., Camacho A., Rodriguez-Valera F.;
RT "Key roles for freshwater Actinobacteria revealed by deep metagenomic
RT sequencing.";
RL Submitted (MAY-2014) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Critical role in recombination and DNA repair. Helps process
CC Holliday junction intermediates to mature products by catalyzing branch
CC migration. Has a DNA unwinding activity characteristic of a DNA
CC helicase with a 3'- to 5'- polarity. Unwinds branched duplex DNA (Y-
CC DNA). {ECO:0000256|RuleBase:RU363016}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.12;
CC Evidence={ECO:0000256|RuleBase:RU363016};
CC -!- SIMILARITY: Belongs to the helicase family. RecG subfamily.
CC {ECO:0000256|ARBA:ARBA00007504, ECO:0000256|RuleBase:RU363016}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KGA10262.1}.
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DR EMBL; JNSI01000010; KGA10262.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A094PGY1; -.
DR PATRIC; fig|1504322.4.peg.237; -.
DR Proteomes; UP000029310; Unassembled WGS sequence.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003678; F:DNA helicase activity; IEA:InterPro.
DR GO; GO:0006310; P:DNA recombination; IEA:UniProtKB-UniRule.
DR GO; GO:0006281; P:DNA repair; IEA:UniProtKB-UniRule.
DR CDD; cd17992; DEXHc_RecG; 1.
DR CDD; cd04488; RecG_wedge_OBF; 1.
DR Gene3D; 2.40.50.140; Nucleic acid-binding proteins; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR InterPro; IPR004609; ATP-dep_DNA_helicase_RecG.
DR InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR InterPro; IPR014001; Helicase_ATP-bd.
DR InterPro; IPR001650; Helicase_C.
DR InterPro; IPR012340; NA-bd_OB-fold.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR047112; RecG/Mfd.
DR InterPro; IPR045562; RecG_dom3_C.
DR InterPro; IPR033454; RecG_wedge.
DR NCBIfam; TIGR00643; recG; 1.
DR PANTHER; PTHR47964; ATP-DEPENDENT DNA HELICASE HOMOLOG RECG, CHLOROPLASTIC; 1.
DR PANTHER; PTHR47964:SF1; ATP-DEPENDENT DNA HELICASE HOMOLOG RECG, CHLOROPLASTIC; 1.
DR Pfam; PF00270; DEAD; 1.
DR Pfam; PF00271; Helicase_C; 1.
DR Pfam; PF19833; RecG_dom3_C; 1.
DR Pfam; PF17191; RecG_wedge; 1.
DR SMART; SM00487; DEXDc; 1.
DR SMART; SM00490; HELICc; 1.
DR SUPFAM; SSF50249; Nucleic acid-binding proteins; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 2.
DR PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR PROSITE; PS51194; HELICASE_CTER; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|RuleBase:RU363016};
KW DNA damage {ECO:0000256|RuleBase:RU363016};
KW DNA recombination {ECO:0000256|RuleBase:RU363016};
KW DNA repair {ECO:0000256|RuleBase:RU363016};
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125};
KW Helicase {ECO:0000256|ARBA:ARBA00022806, ECO:0000256|RuleBase:RU363016};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|RuleBase:RU363016};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741,
KW ECO:0000256|RuleBase:RU363016}.
FT DOMAIN 278..451
FT /note="Helicase ATP-binding"
FT /evidence="ECO:0000259|PROSITE:PS51192"
FT DOMAIN 474..655
FT /note="Helicase C-terminal"
FT /evidence="ECO:0000259|PROSITE:PS51194"
SQ SEQUENCE 728 AA; 79740 MW; E06B38178EA84CF0 CRC64;
MATLSTRLTN IVGDRTAQVL EKSFGITTIN ELLRHYPRRY VVRGELTDIS TLVADDEVTI
LAEIEAVKLR RANGKNILEV VVTDGSANLS LTFFNQAWRE KDLRAGRVGL FAGKVGVFKG
RRQLAHPDYQ LIPDGDDVDA AVAEFAGKYL PVYPATGKLP SWKIMQCMKL ATDSLDELPD
YLPAQITEQF KYPSLKKAFL DIHQPPDLES AENARQRLTF DEALLLQLLL VQRRDEITKL
STKSRTPNTP KLVAAFEKKL PFKYTAGQIE VNSQIEKDLS NKYPMHRLLQ GEVGSGKTVV
ALRAMLSVVD SSGQAALLAP TEVLAQQHYR TITKILGELA QAGTLQAGEI GTQVELLTGS
LSTAEKKEIH TKLATGQTGI VIGTHALISD GVGFDDLGLV VVDEQHRFGV EQRDALRMKA
KQPPHLLVMT ATPIPRTVAM TVFGDLDIST LKELPSGRVA ITTHVIPVLE KPHFLDRAWQ
RVKEEVAKGH QVYIVAPRIA NPNKKLTERE IAAALLLGEE LLDNEEMVAV EELAPQLASG
ALSGLKIAVL HGKQSSEIKD QTMAAFAAGQ IQVLIATTVI EVGVDVPNAS MMVIMDADRF
GVSQLHQLRG RVGRGSAPGL CLLVSSAQED SPAMQRLNSV AATLDGFELA RIDLEQRKEG
DVLGRSQSGG KSHLRLLRVL RDENLIDQAR EVAVKILKTD PDLSNLPELN AEVEKLKSDE
AARFMDKS
//
