ID   A0A099S4I3_9CLOT        Unreviewed;       747 AA.
AC   A0A099S4I3;
DT   07-JAN-2015, integrated into UniProtKB/TrEMBL.
DT   07-JAN-2015, sequence version 1.
DT   27-MAR-2024, entry version 42.
DE   RecName: Full=DNA 3'-5' helicase {ECO:0000256|ARBA:ARBA00034808};
DE            EC=5.6.2.4 {ECO:0000256|ARBA:ARBA00034808};
GN   ORFNames=DP68_09140 {ECO:0000313|EMBL:KGK87459.1};
OS   Clostridium sp. HMP27.
OC   Bacteria; Bacillota; Clostridia; Eubacteriales; Clostridiaceae;
OC   Clostridium.
OX   NCBI_TaxID=1487921 {ECO:0000313|EMBL:KGK87459.1, ECO:0000313|Proteomes:UP000030009};
RN   [1] {ECO:0000313|EMBL:KGK87459.1, ECO:0000313|Proteomes:UP000030009}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=HMP27 {ECO:0000313|EMBL:KGK87459.1,
RC   ECO:0000313|Proteomes:UP000030009};
RA   Tan B.F., Foght J., Budwill K.;
RT   "Draft genome sequence of Clostridium sp. HMP27 isolated from a coal
RT   degrading methanogenic culture.";
RL   Submitted (APR-2014) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=5.6.2.4;
CC         Evidence={ECO:0000256|ARBA:ARBA00034618};
CC   -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC       whole genome shotgun (WGS) entry which is preliminary data.
CC       {ECO:0000313|EMBL:KGK87459.1}.
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DR   EMBL; JMFY01000018; KGK87459.1; -; Genomic_DNA.
DR   RefSeq; WP_035308719.1; NZ_JMFY01000018.1.
DR   AlphaFoldDB; A0A099S4I3; -.
DR   STRING; 1487921.DP68_09140; -.
DR   eggNOG; COG3973; Bacteria.
DR   Proteomes; UP000030009; Unassembled WGS sequence.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR   GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR   GO; GO:0003678; F:DNA helicase activity; IEA:InterPro.
DR   Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR   InterPro; IPR014017; DNA_helicase_UvrD-like_C.
DR   InterPro; IPR000212; DNA_helicase_UvrD/REP.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR014016; UvrD-like_ATP-bd.
DR   PANTHER; PTHR11070:SF17; DNA HELICASE IV; 1.
DR   PANTHER; PTHR11070; UVRD / RECB / PCRA DNA HELICASE FAMILY MEMBER; 1.
DR   Pfam; PF13361; UvrD_C; 1.
DR   SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR   PROSITE; PS51198; UVRD_HELICASE_ATP_BIND; 1.
PE   4: Predicted;
KW   ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|PROSITE-
KW   ProRule:PRU00560}; Coiled coil {ECO:0000256|SAM:Coils};
KW   Helicase {ECO:0000256|ARBA:ARBA00022806, ECO:0000256|PROSITE-
KW   ProRule:PRU00560};
KW   Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|PROSITE-
KW   ProRule:PRU00560};
KW   Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741, ECO:0000256|PROSITE-
KW   ProRule:PRU00560}; Reference proteome {ECO:0000313|Proteomes:UP000030009}.
FT   DOMAIN          208..593
FT                   /note="UvrD-like helicase ATP-binding"
FT                   /evidence="ECO:0000259|PROSITE:PS51198"
FT   COILED          12..43
FT                   /evidence="ECO:0000256|SAM:Coils"
FT   BINDING         229..236
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000256|PROSITE-ProRule:PRU00560"
SQ   SEQUENCE   747 AA;  86743 MW;  AF691D2021B6FE3C CRC64;
     MNTFNENQHE ELEYLEKTLS LIKKNLQEEK ELLNSKLSSV IAAGREMWEE VSHSSENFDE
     VPERNHFLNE SRIKARDYIS TENQIKKLNK LLNSPYFGRF DFIEDDFDDT EKIYVGLSSL
     VDNETSDILV YDWRAPISSI YYRSELGKCF YKSPAGIVSG NILLKRQYKI KDSKLKYFFD
     SSIKINDEML QEVLSQNASD KMKTIVQTIQ KEQDIIIRDT ENELLIVQGA AGSGKTSIAL
     HRIAFLLYEG LNSKLNTNNV IIVSPNSFFS SYISDVLPEL GEDNVEQITF DNIIVNSFHK
     NYVLETRENQ MELLLTLQSC SSDDFKTKLD SIKFKGSPEF IKILDNLLIY LERKLIPFKD
     VYFNGKIIKT GQQIKSIFLN NKINMPAAKR LKRIENMILD KIHPLRKERL EMVEEVVERV
     TEDHILEIKS FSRLLCIKEA AKLMEYIHTF TEIDHLNLYN LLFKDKNLFL RLSKGITLPE
     NIDEIMKYTK GNLDNEAISY EDAAAILYLK LSIQGNEEFG EIKQVVIDEA QDYYPMHYYL
     FNLLFKNARY TVLGDYNQTL EKYGNKTIYD CIAQILKKKK TVKLSLNKSY RSSFEINTFN
     QRLLSNTQEA VPFQRHEAAP EIKFVKDTNE LDTAICKDVT KYFDLNYKSI AVICKSSKEA
     LDVKNRLSKY IDATLLEDKD MKKNDSVLVV PSYLAKGLEF DVVLVYNVSD KNYKSDFDKR
     LLYIACTRAL HGLTLYHTGE KSNFIKD
//