ID A0A0A0KMB2_CUCSA Unreviewed; 750 AA.
AC A0A0A0KMB2;
DT 07-JAN-2015, integrated into UniProtKB/TrEMBL.
DT 07-JAN-2015, sequence version 1.
DT 24-JAN-2024, entry version 44.
DE RecName: Full=beta-galactosidase {ECO:0000256|ARBA:ARBA00012756};
DE EC=3.2.1.23 {ECO:0000256|ARBA:ARBA00012756};
GN ORFNames=Csa_6G504610 {ECO:0000313|EMBL:KGN48881.1};
OS Cucumis sativus (Cucumber).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; fabids; Cucurbitales; Cucurbitaceae; Benincaseae; Cucumis.
OX NCBI_TaxID=3659 {ECO:0000313|EMBL:KGN48881.1, ECO:0000313|Proteomes:UP000029981};
RN [1] {ECO:0000313|EMBL:KGN48881.1, ECO:0000313|Proteomes:UP000029981}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. 9930 {ECO:0000313|Proteomes:UP000029981};
RX PubMed=19881527; DOI=10.1038/ng.475;
RA Huang S., Li R., Zhang Z., Li L., Gu X., Fan W., Lucas W.J., Wang X.,
RA Xie B., Ni P., Ren Y., Zhu H., Li J., Lin K., Jin W., Fei Z., Li G.,
RA Staub J., Kilian A., van der Vossen E.A., Wu Y., Guo J., He J., Jia Z.,
RA Ren Y., Tian G., Lu Y., Ruan J., Qian W., Wang M., Huang Q., Li B.,
RA Xuan Z., Cao J., Asan null, Wu Z., Zhang J., Cai Q., Bai Y., Zhao B.,
RA Han Y., Li Y., Li X., Wang S., Shi Q., Liu S., Cho W.K., Kim J.Y., Xu Y.,
RA Heller-Uszynska K., Miao H., Cheng Z., Zhang S., Wu J., Yang Y., Kang H.,
RA Li M., Liang H., Ren X., Shi Z., Wen M., Jian M., Yang H., Zhang G.,
RA Yang Z., Chen R., Liu S., Li J., Ma L., Liu H., Zhou Y., Zhao J., Fang X.,
RA Li G., Fang L., Li Y., Liu D., Zheng H., Zhang Y., Qin N., Li Z., Yang G.,
RA Yang S., Bolund L., Kristiansen K., Zheng H., Li S., Zhang X., Yang H.,
RA Wang J., Sun R., Zhang B., Jiang S., Wang J., Du Y., Li S.;
RT "The genome of the cucumber, Cucumis sativus L.";
RL Nat. Genet. 41:1275-1281(2009).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Hydrolysis of terminal non-reducing beta-D-galactose residues
CC in beta-D-galactosides.; EC=3.2.1.23;
CC Evidence={ECO:0000256|ARBA:ARBA00001412};
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 35 family.
CC {ECO:0000256|ARBA:ARBA00009809, ECO:0000256|RuleBase:RU003679}.
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DR EMBL; CM002927; KGN48881.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A0A0KMB2; -.
DR EnsemblPlants; KGN48881; KGN48881; Csa_6G504610.
DR Gramene; KGN48881; KGN48881; Csa_6G504610.
DR OMA; ETTGHAM; -.
DR Proteomes; UP000029981; Chromosome 6.
DR GO; GO:0005773; C:vacuole; IBA:GO_Central.
DR GO; GO:0004565; F:beta-galactosidase activity; IBA:GO_Central.
DR GO; GO:0030246; F:carbohydrate binding; IEA:InterPro.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR CDD; cd22842; Gal_Rha_Lectin_BGal; 1.
DR Gene3D; 2.60.120.740; -; 1.
DR Gene3D; 3.20.20.80; Glycosidases; 2.
DR InterPro; IPR048913; BetaGal_gal-bd.
DR InterPro; IPR008979; Galactose-bd-like_sf.
DR InterPro; IPR041392; GHD.
DR InterPro; IPR031330; Gly_Hdrlase_35_cat.
DR InterPro; IPR001944; Glycoside_Hdrlase_35.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR InterPro; IPR000922; Lectin_gal-bd_dom.
DR InterPro; IPR043159; Lectin_gal-bd_sf.
DR PANTHER; PTHR23421:SF79; BETA-GALACTOSIDASE 3; 1.
DR PANTHER; PTHR23421; BETA-GALACTOSIDASE RELATED; 1.
DR Pfam; PF21467; BetaGal_gal-bd; 1.
DR Pfam; PF02140; Gal_Lectin; 1.
DR Pfam; PF17834; GHD; 1.
DR Pfam; PF01301; Glyco_hydro_35; 1.
DR PRINTS; PR00742; GLHYDRLASE35.
DR SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR SUPFAM; SSF49785; Galactose-binding domain-like; 2.
DR PROSITE; PS50228; SUEL_LECTIN; 1.
PE 3: Inferred from homology;
KW Glycosidase {ECO:0000256|ARBA:ARBA00023295};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW Reference proteome {ECO:0000313|Proteomes:UP000029981};
KW Signal {ECO:0000256|SAM:SignalP}.
FT SIGNAL 1..27
FT /evidence="ECO:0000256|SAM:SignalP"
FT CHAIN 28..750
FT /note="beta-galactosidase"
FT /evidence="ECO:0000256|SAM:SignalP"
FT /id="PRO_5001965262"
FT DOMAIN 652..738
FT /note="SUEL-type lectin"
FT /evidence="ECO:0000259|PROSITE:PS50228"
SQ SEQUENCE 750 AA; 83271 MW; E42FF92F26822373 CRC64;
MATNSVSKLS MLVLGLFWLL GVQFVQCSVT YDRKAILING QRRVLFSGSI HYPRSTPEMW
EGLIQKAKEG GLDVVETYVF WNVHEPSPGN YNFEGRYDLV RFIKTIQKAG LYANLRIGPY
VCAEWNFGGF PVWLKYVPGI SFRTDNEPFK RAMQGFTEKI VGLMKSENLF ESQGGPIILS
QINTCNGFYC DAFSPNRPYK PTMWTEAWSG WFNEFGGPIH QRPVQDLAFA VALFIQKGGS
FINYYMYHGG TNFGRTAGGP FITTSYDYDA PIDEYGLIRQ PKYGHLKELH RAVKMCEKAL
VSADPIVTSL GSSQQAYVYT SESGNCAAFL SNYDTDSAAR VMFNNMHYNL PPWSISILPD
CRNVVFNTAK VGVQTSQLEM LPTNSPMLLW ESYNEDVSAE DDSTTMTASG LLEQINVTKD
TSDYLWYITS VDIGSTESFL HGGELPTLIV QSTGHAVHIF INGRLSGSAF GSRENRRFTY
TGKVNFRAGR NTIALLSVAV GLPNVGGHFE TWNTGILGPV ALHGLDQGKL DLSWAKWTYK
VGLKGEAMNL VSPNGISSVE WMEGSLAAQA PQPLTWHKCQ QGCGQPTQRW YHVPRAWLKP
KDNLLVVFEE LGGNPTSISL VKRSVTGVCA DVSEYHPTLK NWHIESYGKS EDLHRPKVHL
KCSAGYSITS IKFASFGTPL GTCGSYQQGT CHAPMSYDIL EKRCIGKQRC AVTISNTNFG
QDPCPNVLKR LSVEVVCAPA TTAAEPNWRG
//
