ID A0A0A1HTQ3_9PSED Unreviewed; 389 AA.
AC A0A0A1HTQ3;
DT 04-FEB-2015, integrated into UniProtKB/TrEMBL.
DT 04-FEB-2015, sequence version 1.
DT 24-JAN-2024, entry version 41.
DE SubName: Full=Mandelate racemase/muconate lactonizing enzyme family protein {ECO:0000313|EMBL:CDF93630.1};
GN ORFNames=BN844_0210 {ECO:0000313|EMBL:CDF93630.1};
OS Pseudomonas sp. SHC52.
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
OC Pseudomonadaceae; Pseudomonas.
OX NCBI_TaxID=984195 {ECO:0000313|EMBL:CDF93630.1, ECO:0000313|Proteomes:UP000031550};
RN [1] {ECO:0000313|EMBL:CDF93630.1, ECO:0000313|Proteomes:UP000031550}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=SHC52 {ECO:0000313|EMBL:CDF93630.1,
RC ECO:0000313|Proteomes:UP000031550};
RA Van der Voort M., Mendes R., Raaijmakers J.M.;
RT "Genome mining of the rhizosphere bacterium Pseudomonas sp. SH-C52.";
RL Submitted (MAY-2013) to the EMBL/GenBank/DDBJ databases.
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000256|PIRSR:PIRSR634611-3};
CC Note=Binds 1 Mg(2+) ion per subunit. {ECO:0000256|PIRSR:PIRSR634611-3};
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:CDF93630.1}.
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DR EMBL; CBLV010000130; CDF93630.1; -; Genomic_DNA.
DR RefSeq; WP_041021420.1; NZ_CBLV010000130.1.
DR AlphaFoldDB; A0A0A1HTQ3; -.
DR OrthoDB; 9802699at2; -.
DR Proteomes; UP000031550; Unassembled WGS sequence.
DR GO; GO:0047808; F:D(-)-tartrate dehydratase activity; IEA:InterPro.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR CDD; cd03326; MR_like_1; 1.
DR Gene3D; 3.20.20.120; Enolase-like C-terminal domain; 1.
DR Gene3D; 3.30.390.10; Enolase-like, N-terminal domain; 1.
DR InterPro; IPR034611; D-tartrate_dehydratase.
DR InterPro; IPR034593; DgoD-like.
DR InterPro; IPR036849; Enolase-like_C_sf.
DR InterPro; IPR029017; Enolase-like_N.
DR InterPro; IPR029065; Enolase_C-like.
DR InterPro; IPR013342; Mandelate_racemase_C.
DR PANTHER; PTHR48080:SF5; D(-)-TARTRATE DEHYDRATASE; 1.
DR PANTHER; PTHR48080; D-GALACTONATE DEHYDRATASE-RELATED; 1.
DR Pfam; PF13378; MR_MLE_C; 1.
DR SFLD; SFLDF00118; D-tartrate_dehydratase; 1.
DR SFLD; SFLDG00179; mandelate_racemase; 1.
DR SMART; SM00922; MR_MLE; 1.
DR SUPFAM; SSF51604; Enolase C-terminal domain-like; 1.
DR SUPFAM; SSF54826; Enolase N-terminal domain-like; 1.
PE 4: Predicted;
KW Magnesium {ECO:0000256|PIRSR:PIRSR634611-3};
KW Metal-binding {ECO:0000256|PIRSR:PIRSR634611-3}.
FT DOMAIN 162..259
FT /note="Mandelate racemase/muconate lactonizing enzyme C-
FT terminal"
FT /evidence="ECO:0000259|SMART:SM00922"
FT ACT_SITE 183
FT /note="acceptor"
FT /evidence="ECO:0000256|PIRSR:PIRSR634611-1"
FT ACT_SITE 321
FT /note="Proton donor/acceptor"
FT /evidence="ECO:0000256|PIRSR:PIRSR634611-1"
FT BINDING 19
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR634611-2"
FT BINDING 53
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR634611-2"
FT BINDING 100
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR634611-2"
FT BINDING 155
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR634611-2"
FT BINDING 181
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR634611-2"
FT BINDING 212
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000256|PIRSR:PIRSR634611-3"
FT BINDING 238
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000256|PIRSR:PIRSR634611-3"
FT BINDING 238
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR634611-2"
FT BINDING 264
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000256|PIRSR:PIRSR634611-3"
FT BINDING 264
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR634611-2"
FT BINDING 321
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR634611-2"
FT SITE 53
FT /note="Transition state stabilizer"
FT /evidence="ECO:0000256|PIRSR:PIRSR634611-4"
FT SITE 181
FT /note="Transition state stabilizer"
FT /evidence="ECO:0000256|PIRSR:PIRSR634611-4"
FT SITE 291
FT /note="Increases basicity of active site His"
FT /evidence="ECO:0000256|PIRSR:PIRSR634611-4"
FT SITE 340
FT /note="Transition state stabilizer"
FT /evidence="ECO:0000256|PIRSR:PIRSR634611-4"
SQ SEQUENCE 389 AA; 43128 MW; A38343C31150B976 CRC64;
MRIVDIREQT VSIASPIANA YIDFSKMTCS VVAVITDVIR DGKPVIGYGF NSNGRYGQGA
LMRDRFLARV TEADPDSLVD HENNNLDPFA IWKTLMTNEK PGGHGERSVA VGTLDMAVWD
AVAKIEGKPL YRLLADRYRG GVADDKVWVY AAGGYYYPGK DQTKLKAEMQ SYLDRGYDVV
KMKIGAVPLD EDIRRIEAVL EVVGDGRRLA VDANGRFDLQ TGIAYAEAIK KYNLFWYEEV
GDPLDYALQA ELANHYELPM ATGENLFSHQ DARNLLRHGG MRPDRDYLQF DCALSYGLVE
YMRTLKVMEE MGWSSRRVVP HGGHQMSLNI AAGLHLGGNE SYPDVFQPFG GFADGIRVEN
GYVGLPDIPG VGFEAKSALY AVMRALGEG
//
