ID A0A0A7LGY5_9BACT Unreviewed; 669 AA.
AC A0A0A7LGY5;
DT 04-MAR-2015, integrated into UniProtKB/TrEMBL.
DT 04-MAR-2015, sequence version 1.
DT 27-MAR-2024, entry version 29.
DE RecName: Full=pullulanase {ECO:0000256|ARBA:ARBA00024062};
DE EC=3.2.1.41 {ECO:0000256|ARBA:ARBA00024062};
DE AltName: Full=Alpha-dextrin endo-1,6-alpha-glucosidase {ECO:0000256|ARBA:ARBA00029618};
DE AltName: Full=Pullulan 6-glucanohydrolase {ECO:0000256|ARBA:ARBA00031076};
GN ORFNames=PK28_00830 {ECO:0000313|EMBL:AIZ62591.1};
OS Hymenobacter sp. DG25B.
OC Bacteria; Bacteroidota; Cytophagia; Cytophagales; Hymenobacteraceae;
OC Hymenobacter.
OX NCBI_TaxID=1385664 {ECO:0000313|EMBL:AIZ62591.1, ECO:0000313|Proteomes:UP000030789};
RN [1] {ECO:0000313|EMBL:AIZ62591.1, ECO:0000313|Proteomes:UP000030789}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DG25B {ECO:0000313|EMBL:AIZ62591.1,
RC ECO:0000313|Proteomes:UP000030789};
RA Jung H.-Y., Kim M.K., Srinivasan S., Lim S.;
RT "Hymenobacter radioresistens genome sequence.";
RL Submitted (NOV-2014) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Hydrolysis of (1->6)-alpha-D-glucosidic linkages in pullulan,
CC amylopectin and glycogen, and in the alpha- and beta-limit dextrins
CC of amylopectin and glycogen.; EC=3.2.1.41;
CC Evidence={ECO:0000256|ARBA:ARBA00023965};
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DR EMBL; CP010054; AIZ62591.1; -; Genomic_DNA.
DR RefSeq; WP_044510465.1; NZ_CP010054.1.
DR AlphaFoldDB; A0A0A7LGY5; -.
DR STRING; 1385664.PK28_00830; -.
DR KEGG; hyd:PK28_00830; -.
DR HOGENOM; CLU_004744_4_1_10; -.
DR OrthoDB; 9761875at2; -.
DR Proteomes; UP000030789; Chromosome.
DR GO; GO:0004553; F:hydrolase activity, hydrolyzing O-glycosyl compounds; IEA:InterPro.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR CDD; cd11341; AmyAc_Pullulanase_LD-like; 1.
DR CDD; cd02860; E_set_Pullulanase; 1.
DR Gene3D; 3.20.20.80; Glycosidases; 1.
DR Gene3D; 2.60.40.1180; Golgi alpha-mannosidase II; 1.
DR Gene3D; 2.60.40.10; Immunoglobulins; 1.
DR InterPro; IPR006047; Glyco_hydro_13_cat_dom.
DR InterPro; IPR004193; Glyco_hydro_13_N.
DR InterPro; IPR013780; Glyco_hydro_b.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR InterPro; IPR013783; Ig-like_fold.
DR InterPro; IPR014756; Ig_E-set.
DR InterPro; IPR049117; pulA_all-beta.
DR InterPro; IPR011840; PulA_typeI.
DR NCBIfam; TIGR02104; pulA_typeI; 1.
DR PANTHER; PTHR43002; GLYCOGEN DEBRANCHING ENZYME; 1.
DR PANTHER; PTHR43002:SF11; PULLULANASE 1, CHLOROPLASTIC; 1.
DR Pfam; PF00128; Alpha-amylase; 1.
DR Pfam; PF02922; CBM_48; 1.
DR Pfam; PF21653; pulA_all-beta; 1.
DR SMART; SM00642; Aamy; 1.
DR SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR SUPFAM; SSF81296; E set domains; 1.
PE 4: Predicted;
KW Reference proteome {ECO:0000313|Proteomes:UP000030789}.
FT DOMAIN 185..565
FT /note="Glycosyl hydrolase family 13 catalytic"
FT /evidence="ECO:0000259|SMART:SM00642"
SQ SEQUENCE 669 AA; 74018 MW; 13B95549670335EB CRC64;
MKSWLLVTLF ASLTTLTTCR TALPTDTYEA YPTYSGPDLG LTFVQGHARL RVWAPTAEKL
QLKLYAEGTG GTPVAEYAMQ KSMGGTWVYA LPAQPAGRFY VVQATIKGRQ MAETPDPYVH
AVGLNGQRGA LLDPATVRPL EWEDDVRPKL RQATDIIIGE LHVRDLSMQP QSGIQHKGKF
LGVAEIGTRG PEGVKTGLDH LSELGITHVH LLPTNDYASI DESQLQENRY NWGYDPLNYS
VPEGSYATDP ADPAVRILEM KQMVQRLHRR GLRLVLDVVY NHTANAARSS FEQLVPGYYY
RHNADGSLSD AAACGNEVAS ERPMVRKLIV ESVAYWAREY HVDGFRFDLM GILDIRTMRA
VRTALDETDH SIFVYGEGWT AGASPLPEAQ RAVKANVLKM DRIAAFGDEL RDGVKGHYAR
QTEPGFASGQ PGLEESVKFG IVAATQHPQL DYSKVNYSKT PWATTPAQAI NYVACHDDRV
LWDKLTVANP GASEAELIRM DLLSNTIVFT SQGVPFLPVG DEFLRTKGGS HNSYNLPDNV
NQLDWARKAK YQEVFRFYQQ LIALRKAHPA FRLPTQELIQ QHLEFLPGLP ATTIGYRLLH
HAGGDEWNTI VVLFNGSRQP ANLPLPGGRY SVVLAGDQIN PQGISKLEST AAEMKVEVPA
SSAMILVQP
//
