ID A0A0B0N5L8_GOSAR Unreviewed; 483 AA.
AC A0A0B0N5L8;
DT 04-MAR-2015, integrated into UniProtKB/TrEMBL.
DT 04-MAR-2015, sequence version 1.
DT 27-MAR-2024, entry version 29.
DE SubName: Full=tRNA (Guanine(10)-N2)-methyltransferase {ECO:0000313|EMBL:KHG07972.1};
GN ORFNames=F383_16053 {ECO:0000313|EMBL:KHG07972.1};
OS Gossypium arboreum (Tree cotton) (Gossypium nanking).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Malvales; Malvaceae; Malvoideae; Gossypium.
OX NCBI_TaxID=29729 {ECO:0000313|EMBL:KHG07972.1, ECO:0000313|Proteomes:UP000032142};
RN [1] {ECO:0000313|Proteomes:UP000032142}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=cv. AKA8401 {ECO:0000313|Proteomes:UP000032142};
RA Mudge J., Ramaraj T., Lindquist I.E., Bharti A.K., Sundararajan A.,
RA Cameron C.T., Woodward J.E., May G.D., Brubaker C., Broadhvest J.,
RA Wilkins T.A.;
RL Submitted (SEP-2014) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Catalytic subunit of an S-adenosyl-L-methionine-dependent
CC tRNA methyltransferase complex that mediates the methylation of the
CC guanosine nucleotide at position 10 (m2G10) in tRNAs.
CC {ECO:0000256|ARBA:ARBA00002425}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KHG07972.1}.
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DR EMBL; JRRC01486362; KHG07972.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A0B0N5L8; -.
DR Proteomes; UP000032142; Unassembled WGS sequence.
DR GO; GO:0008168; F:methyltransferase activity; IEA:UniProtKB-KW.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR GO; GO:0032259; P:methylation; IEA:UniProtKB-KW.
DR GO; GO:0009451; P:RNA modification; IEA:UniProt.
DR GO; GO:0008033; P:tRNA processing; IEA:UniProtKB-KW.
DR Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 1.
DR InterPro; IPR002052; DNA_methylase_N6_adenine_CS.
DR InterPro; IPR000241; RlmKL-like_Mtase.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR InterPro; IPR016691; tRNA_mtfrase_TRM11.
DR PANTHER; PTHR13370; RNA METHYLASE-RELATED; 1.
DR PANTHER; PTHR13370:SF3; TRNA (GUANINE(10)-N2)-METHYLTRANSFERASE HOMOLOG; 1.
DR Pfam; PF01170; UPF0020; 1.
DR PIRSF; PIRSF017259; tRNA_mtfrase_TRM11; 1.
DR SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 1.
DR PROSITE; PS00092; N6_MTASE; 1.
DR PROSITE; PS51627; SAM_MT_TRM11; 1.
PE 4: Predicted;
KW Methyltransferase {ECO:0000256|ARBA:ARBA00022603,
KW ECO:0000313|EMBL:KHG07972.1};
KW Reference proteome {ECO:0000313|Proteomes:UP000032142};
KW RNA-binding {ECO:0000256|ARBA:ARBA00022555};
KW S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000313|EMBL:KHG07972.1};
KW tRNA-binding {ECO:0000256|ARBA:ARBA00022555}.
FT DOMAIN 201..323
FT /note="UPF0020"
FT /evidence="ECO:0000259|Pfam:PF01170"
FT REGION 462..483
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 466..483
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 483 AA; 54788 MW; 2FCFD7C0B1A9E7F9 CRC64;
MWYLCVFYHR LLDYRKPEFE SLADLFGAFG EKRSSDFNTQ DKVLQWRLPK HHHPDSPFHF
VHLPSEDVAR NIANRSILVK GIYELWGEGN DFEELEEAVK SYPDERKLPY LESESTFKVT
VDSFGKVLSL QEQNERIQGL SYIPFKGRVN LKNPDHNFWL METDENETNN GLPPVVKRRI
FFGREIGGAD RKLIPLYQLK TRNYLGPTAM DAEMAFLMAN QAQAAPGKLV YDPFVGTGSI
LVSAAHFGAM TMGADIDIRV VRDGRGPDCN VWSNFKQYGL PMPIGLLRAD NNLPPWRPGL
KEVFDAIICD PPYGVRAGGR KSGGRKLLKG IVGPYTVPDD KRADHIPSTA AYTLVECVHD
LLDLAARMLV MGGRLVFFYP VLREDGSVET HFPEHPCFKL VATSEQMLSS RYSRVLLTMV
KTSLYTEELA LAARIKHLEF RENHLKWLEN GNLHSLVFSP ADAESGDSKV SKESKPKYRG
KYV
//
