ID A0A0B1P0U7_UNCNE Unreviewed; 1137 AA.
AC A0A0B1P0U7;
DT 04-MAR-2015, integrated into UniProtKB/TrEMBL.
DT 04-MAR-2015, sequence version 1.
DT 24-JAN-2024, entry version 38.
DE RecName: Full=leucine--tRNA ligase {ECO:0000256|ARBA:ARBA00013164};
DE EC=6.1.1.4 {ECO:0000256|ARBA:ARBA00013164};
DE AltName: Full=Leucyl-tRNA synthetase {ECO:0000256|ARBA:ARBA00030520};
GN ORFNames=EV44_g0878 {ECO:0000313|EMBL:KHJ30546.1};
OS Uncinula necator (Grape powdery mildew).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Leotiomycetes;
OC Erysiphales; Erysiphaceae; Erysiphe.
OX NCBI_TaxID=52586 {ECO:0000313|EMBL:KHJ30546.1, ECO:0000313|Proteomes:UP000030854};
RN [1] {ECO:0000313|EMBL:KHJ30546.1, ECO:0000313|Proteomes:UP000030854}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=c {ECO:0000313|Proteomes:UP000030854};
RX PubMed=25487071;
RA Jones L., Riaz S., Morales-Cruz A., Amrine K.C., McGuire B., Gubler W.D.,
RA Walker M.A., Cantu D.;
RT "Adaptive genomic structural variation in the grape powdery mildew
RT pathogen, Erysiphe necator.";
RL BMC Genomics 15:1081-1081(2014).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-
CC tRNA(Leu); Xref=Rhea:RHEA:11688, Rhea:RHEA-COMP:9613, Rhea:RHEA-
CC COMP:9622, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57427,
CC ChEBI:CHEBI:78442, ChEBI:CHEBI:78494, ChEBI:CHEBI:456215; EC=6.1.1.4;
CC Evidence={ECO:0000256|ARBA:ARBA00001372};
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KHJ30546.1}.
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DR EMBL; JNVN01004022; KHJ30546.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A0B1P0U7; -.
DR STRING; 52586.A0A0B1P0U7; -.
DR HOGENOM; CLU_004174_1_1_1; -.
DR OMA; KFIEWQF; -.
DR OrthoDB; 5472610at2759; -.
DR Proteomes; UP000030854; Unassembled WGS sequence.
DR GO; GO:0002161; F:aminoacyl-tRNA editing activity; IEA:InterPro.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0004823; F:leucine-tRNA ligase activity; IEA:UniProtKB-EC.
DR GO; GO:0006429; P:leucyl-tRNA aminoacylation; IEA:InterPro.
DR CDD; cd07959; Anticodon_Ia_Leu_AEc; 1.
DR CDD; cd00812; LeuRS_core; 1.
DR Gene3D; 3.40.50.620; HUPs; 1.
DR Gene3D; 3.90.740.10; Valyl/Leucyl/Isoleucyl-tRNA synthetase, editing domain; 1.
DR InterPro; IPR002300; aa-tRNA-synth_Ia.
DR InterPro; IPR004493; Leu-tRNA-synth_Ia_arc/euk.
DR InterPro; IPR013155; M/V/L/I-tRNA-synth_anticd-bd.
DR InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR InterPro; IPR009080; tRNAsynth_Ia_anticodon-bd.
DR InterPro; IPR009008; Val/Leu/Ile-tRNA-synth_edit.
DR NCBIfam; TIGR00395; leuS_arch; 1.
DR PANTHER; PTHR45794:SF1; LEUCINE--TRNA LIGASE, CYTOPLASMIC; 1.
DR PANTHER; PTHR45794; LEUCYL-TRNA SYNTHETASE; 1.
DR Pfam; PF08264; Anticodon_1; 1.
DR Pfam; PF00133; tRNA-synt_1; 2.
DR SUPFAM; SSF47323; Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases; 1.
DR SUPFAM; SSF52374; Nucleotidylyl transferase; 1.
DR SUPFAM; SSF50677; ValRS/IleRS/LeuRS editing domain; 1.
PE 4: Predicted;
KW Aminoacyl-tRNA synthetase {ECO:0000256|ARBA:ARBA00023146,
KW ECO:0000313|EMBL:KHJ30546.1}; ATP-binding {ECO:0000256|ARBA:ARBA00022840};
KW Ligase {ECO:0000256|ARBA:ARBA00022598};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW Protein biosynthesis {ECO:0000256|ARBA:ARBA00022917};
KW Reference proteome {ECO:0000313|Proteomes:UP000030854}.
FT DOMAIN 72..135
FT /note="Aminoacyl-tRNA synthetase class Ia"
FT /evidence="ECO:0000259|Pfam:PF00133"
FT DOMAIN 214..795
FT /note="Aminoacyl-tRNA synthetase class Ia"
FT /evidence="ECO:0000259|Pfam:PF00133"
FT DOMAIN 836..949
FT /note="Methionyl/Valyl/Leucyl/Isoleucyl-tRNA synthetase
FT anticodon-binding"
FT /evidence="ECO:0000259|Pfam:PF08264"
SQ SEQUENCE 1137 AA; 128929 MW; AEAA0D0E4B1E967F CRC64;
MAPIGPAPII METTVMSKTK ELKGTEKRDS LISFEKKYQV EWQTKGIFQA NAPSISQTPL
HSISPAELRV QYPKFYGTMA YPYMNGTLHA GHSFTVSKIE FAVGFARMQG KRSLFPMGFH
CTGMPIKASA DKLAAEVAIF GKKFENYKDE EVEIVLKDQT KSDSRREDVT KFTAKKGKAA
TKAVRMKYQF QIMQAIGIPT EEIHQFAEAD HWLDYFPPLC QRDLTNFGSR IDWRRSMVTT
DANPFYDAFV RWQMNRLKEL KKIKFGKRYT IYSPKDGQPC MDHDRSEGEA VGPQEYTALK
LKVLDWAPEA KKIIEGKIPE DADTYFVPAT LRPETMYGQT CCFVGPNIIY GIFRLSSKEF
FIVTERAARN MAYQGIFPEN GVYEKVGELI GTTCVGTLVH APLSLHKDGV RILPMESVLS
TKGTGVVTSV PSDSPDDFAT VTDLCKKAEY YGIKKEWVEL EILPIIRTPT YGDRTAEYLV
KKFKILSPKD TKKLEEAKEL AYKEGYYQGI MLRGDYAGEK VEVAKPKVKQ QLIASGEGFA
YSEPERKVVS RSGDECCVAL MDQWYIDYGE ESWKQIALRH VGNTDGAGMN TFAQETKNGL
EGTLNWLNQW ACARSYGLGS KLPWDPQFLV ESLSDSTIYM AYYTIAHYLH SDIFGKSPGL
ANIKPDQMTD EVFDYIFARR DLTESILLES KIKEEVLASM RREFEYWYPL DVRISGKDLI
PNHLTFFLYI HLAIFPKEYW PRGIRANGHL LLNGEKMAKS TGNFMTLDDM LKKYGADATR
IALADAGDGV TDANFEEDVA DNNILRLFTL REWIQEQVKS ASSLRDGAKN DFLDALFENE
MNTIVCQARQ HYEDTNYKLA LKSALYDFTS ARDFYREYSA AAGVKMHRDL IFKYIELQAL
LLCVIAPHWA EYIWLEVLHK PCTIQNALFP TVPAPNAALN AEREYVRATG GNVTSAEAAQ
QKKKSKGRDI GYDPSKPKKL TIYCADSWPA WQEKYIKLVK SHWDLKKKCM LIDDKELNRQ
VTLMNEIKTN KKVMPFVQSL KKRLITGVED EIQIFERKLP FDEVQTLQNM VSGLKKAASL
AVIEIIRVSS SDGGKSGLVV SVGDSLMNHY DVRKIENLPI LAQSAVPGIP TFGFENV
//
