ID A0A0B4S0J5_9FIRM Unreviewed; 313 AA.
AC A0A0B4S0J5;
DT 01-APR-2015, integrated into UniProtKB/TrEMBL.
DT 01-APR-2015, sequence version 1.
DT 24-JAN-2024, entry version 42.
DE RecName: Full=DNA-directed RNA polymerase subunit alpha {ECO:0000256|ARBA:ARBA00015972, ECO:0000256|HAMAP-Rule:MF_00059};
DE Short=RNAP subunit alpha {ECO:0000256|HAMAP-Rule:MF_00059};
DE EC=2.7.7.6 {ECO:0000256|ARBA:ARBA00012418, ECO:0000256|HAMAP-Rule:MF_00059};
DE AltName: Full=RNA polymerase subunit alpha {ECO:0000256|ARBA:ARBA00033070, ECO:0000256|HAMAP-Rule:MF_00059};
DE AltName: Full=Transcriptase subunit alpha {ECO:0000256|ARBA:ARBA00032524, ECO:0000256|HAMAP-Rule:MF_00059};
GN Name=rpoA {ECO:0000256|HAMAP-Rule:MF_00059};
GN ORFNames=HXM94_02095 {ECO:0000313|EMBL:MBF1306570.1}, NND69_04945
GN {ECO:0000313|EMBL:MCZ7407715.1}, NW74_03035
GN {ECO:0000313|EMBL:AIZ36382.1};
OS Parvimonas micra.
OC Bacteria; Bacillota; Tissierellia; Tissierellales; Peptoniphilaceae;
OC Parvimonas.
OX NCBI_TaxID=33033 {ECO:0000313|EMBL:AIZ36382.1, ECO:0000313|Proteomes:UP000031386};
RN [1] {ECO:0000313|EMBL:AIZ36382.1, ECO:0000313|Proteomes:UP000031386}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=KCOM 1535 {ECO:0000313|EMBL:AIZ36382.1}, and KCOM 1535 / ChDC
RC B708 {ECO:0000313|Proteomes:UP000031386};
RA Kook J.-K., Park S.-N., Lim Y.K., Roh H.;
RT "Complete genome sequence of Parvimonas micra KCOM 1535 (= ChDC B708).";
RL Submitted (OCT-2014) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|EMBL:MBF1306570.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=JCVI_23_bin.11 {ECO:0000313|EMBL:MBF1306570.1};
RA Baker J.L., Morton J.T., Dinis M., Alvarez R., Tran N.C., Knight R.,
RA Edlund A.;
RT "Deep metagenomics examines the oral microbiome during advanced dental
RT caries in children, revealing novel taxa and co-occurrences with host
RT molecules.";
RL Submitted (APR-2020) to the EMBL/GenBank/DDBJ databases.
RN [3] {ECO:0000313|EMBL:MCZ7407715.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=PM79KC-AC-4 {ECO:0000313|EMBL:MCZ7407715.1};
RA Conde-Perez K., Buetas E., Aja-Macaya P., Martin-De Arribas E.,
RA Iglesias-Corras I., Trigo-Tasende N., Nasser-Ali M., Estevez L.S.,
RA Rumbo-Feal S., Otero-Alen B., Noguera J.F., Concha A., Pardinas-Lopez S.,
RA Carda-Dieguez M., Gomez-Randulfe I., Martinez-Lago N., Ladra S.,
RA Aparicio L.A., Bou G., Mira A., Vallejo J.A., Poza M.;
RT "Parvimonas micra travels from the subgingival sulcus of the human oral
RT cavity to the colorectal adenocarcinoma.";
RL Submitted (JUL-2022) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC {ECO:0000256|HAMAP-Rule:MF_00059}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.6; Evidence={ECO:0000256|ARBA:ARBA00024550,
CC ECO:0000256|HAMAP-Rule:MF_00059};
CC -!- SUBUNIT: Homodimer. The RNAP catalytic core consists of 2 alpha, 1
CC beta, 1 beta' and 1 omega subunit. When a sigma factor is associated
CC with the core the holoenzyme is formed, which can initiate
CC transcription. {ECO:0000256|HAMAP-Rule:MF_00059}.
CC -!- DOMAIN: The N-terminal domain is essential for RNAP assembly and basal
CC transcription, whereas the C-terminal domain is involved in interaction
CC with transcriptional regulators and with upstream promoter elements.
CC {ECO:0000256|HAMAP-Rule:MF_00059}.
CC -!- SIMILARITY: Belongs to the RNA polymerase alpha chain family.
CC {ECO:0000256|ARBA:ARBA00007123, ECO:0000256|HAMAP-Rule:MF_00059}.
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DR EMBL; CP009761; AIZ36382.1; -; Genomic_DNA.
DR EMBL; JABZRE010000004; MBF1306570.1; -; Genomic_DNA.
DR EMBL; JANDZV010000003; MCZ7407715.1; -; Genomic_DNA.
DR RefSeq; WP_004833588.1; NZ_RKIS01000001.1.
DR STRING; 33033.NW74_03035; -.
DR KEGG; pmic:NW74_03035; -.
DR OrthoDB; 9805706at2; -.
DR Proteomes; UP000031386; Chromosome.
DR Proteomes; UP000758611; Unassembled WGS sequence.
DR Proteomes; UP001141458; Unassembled WGS sequence.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProt.
DR GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0046983; F:protein dimerization activity; IEA:InterPro.
DR GO; GO:0006351; P:DNA-templated transcription; IEA:UniProtKB-UniRule.
DR CDD; cd06928; RNAP_alpha_NTD; 1.
DR Gene3D; 1.10.150.20; 5' to 3' exonuclease, C-terminal subdomain; 1.
DR Gene3D; 2.170.120.12; DNA-directed RNA polymerase, insert domain; 1.
DR Gene3D; 3.30.1360.10; RNA polymerase, RBP11-like subunit; 1.
DR HAMAP; MF_00059; RNApol_bact_RpoA; 1.
DR InterPro; IPR011262; DNA-dir_RNA_pol_insert.
DR InterPro; IPR011263; DNA-dir_RNA_pol_RpoA/D/Rpb3.
DR InterPro; IPR011773; DNA-dir_RpoA.
DR InterPro; IPR036603; RBP11-like.
DR InterPro; IPR011260; RNAP_asu_C.
DR InterPro; IPR036643; RNApol_insert_sf.
DR NCBIfam; TIGR02027; rpoA; 1.
DR Pfam; PF01000; RNA_pol_A_bac; 1.
DR Pfam; PF03118; RNA_pol_A_CTD; 1.
DR Pfam; PF01193; RNA_pol_L; 1.
DR SMART; SM00662; RPOLD; 1.
DR SUPFAM; SSF47789; C-terminal domain of RNA polymerase alpha subunit; 1.
DR SUPFAM; SSF56553; Insert subdomain of RNA polymerase alpha subunit; 1.
DR SUPFAM; SSF55257; RBP11-like subunits of RNA polymerase; 1.
PE 3: Inferred from homology;
KW DNA-directed RNA polymerase {ECO:0000256|ARBA:ARBA00022478,
KW ECO:0000256|HAMAP-Rule:MF_00059};
KW Nucleotidyltransferase {ECO:0000256|ARBA:ARBA00022695, ECO:0000256|HAMAP-
KW Rule:MF_00059}; Reference proteome {ECO:0000313|Proteomes:UP000031386};
KW Transcription {ECO:0000256|ARBA:ARBA00023163, ECO:0000256|HAMAP-
KW Rule:MF_00059};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|HAMAP-
KW Rule:MF_00059}.
FT DOMAIN 20..226
FT /note="DNA-directed RNA polymerase RpoA/D/Rpb3-type"
FT /evidence="ECO:0000259|SMART:SM00662"
FT REGION 1..232
FT /note="Alpha N-terminal domain (alpha-NTD)"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_00059"
FT REGION 242..313
FT /note="Alpha C-terminal domain (alpha-CTD)"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_00059"
SQ SEQUENCE 313 AA; 34546 MW; 7F7D32B9DA81BC0D CRC64;
MTEDLNTNIE VLDIDLEKNY GKFAIMPLDR GYGTTLGNSM RRVLLGSLPG SAIATVKIDG
VGHEFTTIKG SIADVPEILI NIKGIALKKF NDEPVILELN VKGPKVVTAG DISENQNVEI
ANKDHYITTL NEEGEIRMDL LVLNGKGYRV SDLNKEYCDI NSIAVDSSFS PVKKVNFIVG
NTRVGQSIDF DKLEIEVWTN GTITPQEALS TGAGIFIDYL SLFRSLPDIN LDNSSIKDGK
ILDANGVLSK SVEELDLSLR SFNCLKRANL DTVKDIISQD IEQLKKIKNF GKKSVEEVVD
KIHELGLKFL NEE
//
