ID A0A0B8NZL8_9VIBR Unreviewed; 686 AA.
AC A0A0B8NZL8;
DT 01-APR-2015, integrated into UniProtKB/TrEMBL.
DT 01-APR-2015, sequence version 1.
DT 24-JAN-2024, entry version 27.
DE SubName: Full=Periplasmic alpha-amylase {ECO:0000313|EMBL:GAM56463.1};
DE EC=3.2.1.1 {ECO:0000313|EMBL:GAM56463.1};
GN ORFNames=JCM19231_290 {ECO:0000313|EMBL:GAM56463.1};
OS Vibrio ishigakensis.
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Vibrionales; Vibrionaceae;
OC Vibrio.
OX NCBI_TaxID=1481914 {ECO:0000313|EMBL:GAM56463.1, ECO:0000313|Proteomes:UP000031671};
RN [1] {ECO:0000313|EMBL:GAM56463.1, ECO:0000313|Proteomes:UP000031671}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=JCM 19231 {ECO:0000313|Proteomes:UP000031671};
RA Sawabe T., Meirelles P., Feng G., Sayaka M., Hattori M., Ohkuma M.;
RT "Vibrio sp. C1 JCM 19231 whole genome shotgun sequence.";
RL Submitted (JAN-2015) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|EMBL:GAM56463.1, ECO:0000313|Proteomes:UP000031671}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=JCM 19231 {ECO:0000313|Proteomes:UP000031671};
RG NBRP consortium;
RA Sawabe T., Meirelles P., Feng G., Sayaka M., Hattori M., Ohkuma M.;
RL Submitted (JAN-2015) to the EMBL/GenBank/DDBJ databases.
CC -!- COFACTOR:
CC Name=Ca(2+); Xref=ChEBI:CHEBI:29108;
CC Evidence={ECO:0000256|PIRSR:PIRSR036917-2};
CC Note=Binds 1 Ca(2+) ion per subunit. {ECO:0000256|PIRSR:PIRSR036917-2};
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:GAM56463.1}.
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DR EMBL; BBRZ01000030; GAM56463.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A0B8NZL8; -.
DR Proteomes; UP000031671; Unassembled WGS sequence.
DR GO; GO:0042597; C:periplasmic space; IEA:InterPro.
DR GO; GO:0004556; F:alpha-amylase activity; IEA:UniProtKB-EC.
DR GO; GO:0005509; F:calcium ion binding; IEA:InterPro.
DR GO; GO:0030980; P:alpha-glucan catabolic process; IEA:InterPro.
DR GO; GO:0009313; P:oligosaccharide catabolic process; IEA:InterPro.
DR Gene3D; 3.20.20.80; Glycosidases; 2.
DR InterPro; IPR014635; A_amylase_MalS.
DR InterPro; IPR006047; Glyco_hydro_13_cat_dom.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR PANTHER; PTHR10357; ALPHA-AMYLASE FAMILY MEMBER; 1.
DR PANTHER; PTHR10357:SF209; PERIPLASMIC ALPHA-AMYLASE; 1.
DR Pfam; PF00128; Alpha-amylase; 2.
DR PIRSF; PIRSF036917; Alph_amls_MalS; 1.
DR SMART; SM00642; Aamy; 1.
DR SUPFAM; SSF51445; (Trans)glycosidases; 1.
PE 4: Predicted;
KW Calcium {ECO:0000256|PIRSR:PIRSR036917-2};
KW Disulfide bond {ECO:0000256|PIRSR:PIRSR036917-4};
KW Glycosidase {ECO:0000313|EMBL:GAM56463.1};
KW Hydrolase {ECO:0000313|EMBL:GAM56463.1};
KW Metal-binding {ECO:0000256|PIRSR:PIRSR036917-2};
KW Reference proteome {ECO:0000313|Proteomes:UP000031671};
KW Signal {ECO:0000256|SAM:SignalP}.
FT SIGNAL 1..21
FT /evidence="ECO:0000256|SAM:SignalP"
FT CHAIN 22..686
FT /evidence="ECO:0000256|SAM:SignalP"
FT /id="PRO_5002139574"
FT DOMAIN 206..648
FT /note="Glycosyl hydrolase family 13 catalytic"
FT /evidence="ECO:0000259|SMART:SM00642"
FT ACT_SITE 466
FT /note="Nucleophile"
FT /evidence="ECO:0000256|PIRSR:PIRSR036917-1"
FT ACT_SITE 506
FT /note="Proton donor"
FT /evidence="ECO:0000256|PIRSR:PIRSR036917-1"
FT BINDING 327
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000256|PIRSR:PIRSR036917-2"
FT BINDING 470
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000256|PIRSR:PIRSR036917-2"
FT SITE 571
FT /note="Transition state stabilizer"
FT /evidence="ECO:0000256|PIRSR:PIRSR036917-3"
FT DISULFID 132..540
FT /evidence="ECO:0000256|PIRSR:PIRSR036917-4"
SQ SEQUENCE 686 AA; 76824 MW; 808719C57BFE4430 CRC64;
MKLTQIGLLV AGICASGSLL AEPNITISTS TTSRDFPISA DQPLVVPLNK EQYIFKVTGI
EGECSAPEEQ KVRFNRPLGI NCGADTELPF NIRFAGDYAI TYDDQAQTIS VKRQPKKAAK
KEFVRPIPQV SCEVFTDKPV SIDVAGTFQD GTELVESFSG QTAEVKDGKV SLNPSRSNGG
VMLLEKAGSE PSKEEALDWR NANIYFVMVD RFNNGDSSND QSYGRQKDGK EEIGTFHGGD
LKGVIEKLDY IQSLGTDAIW LSPIVEQVHG FVGGGNKGSF PFYGYHGYWA RDFTKIDQNF
GNDEDLKTLV TEAHKRGIKV MLDAVVNHPG YATVADIQHD KLNVLKGSAK GQDLTQWKPE
PGKNWHSVND LVDYQNDNWS QWWGKDWVRT GLPGYDSPGS SDKTLTLAGL PDFKTESEKL
VTPPQWLIDN PGTRVVAKEN YRVADYLVEW QTDWVKRFGI DAFRVDTVKH VEGEVWLDLK
AKASERLAEW QKANNRKQEP FWMMGEVWGH SPYRSPYADE GFDALINFDM QKKLDKGAAC
FSSMSDTYAN YSKEIQDNPG YTPVSYMSSH DTELFFSRFK SFEMQRDAAS ALLLSPGAIQ
VYYGDEVARD IGPYADDFHQ GTRSDMVWKL DKDREALLDH WQTLGQFRQR HPSVGGGVHT
DLAQEHGFAF SRTLDDDAVV VYFAGK
//