ID A0A0C9PJU6_9BACT Unreviewed; 493 AA.
AC A0A0C9PJU6;
DT 29-APR-2015, integrated into UniProtKB/TrEMBL.
DT 29-APR-2015, sequence version 1.
DT 27-MAR-2024, entry version 32.
DE SubName: Full=Radical SAM domain protein {ECO:0000313|EMBL:GAN32079.1};
GN ORFNames=BROSI_A0583 {ECO:0000313|EMBL:GAN32079.1};
OS Candidatus Brocadia sinica JPN1.
OC Bacteria; Planctomycetota; Candidatus Brocadiia; Candidatus Brocadiales;
OC Candidatus Brocadiaceae; Brocadia.
OX NCBI_TaxID=1197129 {ECO:0000313|EMBL:GAN32079.1, ECO:0000313|Proteomes:UP000032309};
RN [1] {ECO:0000313|EMBL:GAN32079.1, ECO:0000313|Proteomes:UP000032309}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=JPN1 {ECO:0000313|EMBL:GAN32079.1,
RC ECO:0000313|Proteomes:UP000032309};
RA Oshiki M., Shinyako-Hata K., Satoh H., Okabe S.;
RT "Draft Genome Sequence of an Anaerobic Ammonium-Oxidizing Bacterium,
RT "Candidatus Brocadia sinica".";
RL Genome Announc. 3:e00267-15(2015).
CC -!- COFACTOR:
CC Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC Evidence={ECO:0000256|ARBA:ARBA00001966};
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:GAN32079.1}.
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DR EMBL; BAFN01000001; GAN32079.1; -; Genomic_DNA.
DR RefSeq; WP_052562208.1; NZ_BAFN01000001.1.
DR AlphaFoldDB; A0A0C9PJU6; -.
DR OrthoDB; 226469at2; -.
DR Proteomes; UP000032309; Unassembled WGS sequence.
DR GO; GO:0003824; F:catalytic activity; IEA:InterPro.
DR GO; GO:0031419; F:cobalamin binding; IEA:InterPro.
DR GO; GO:0051536; F:iron-sulfur cluster binding; IEA:InterPro.
DR GO; GO:0046872; F:metal ion binding; IEA:InterPro.
DR CDD; cd01335; Radical_SAM; 1.
DR CDD; cd02068; radical_SAM_B12_BD; 1.
DR Gene3D; 3.40.50.280; Cobalamin-binding domain; 1.
DR Gene3D; 3.80.30.20; tm_1862 like domain; 1.
DR InterPro; IPR006158; Cobalamin-bd.
DR InterPro; IPR025274; DUF4070.
DR InterPro; IPR006638; Elp3/MiaA/NifB-like_rSAM.
DR InterPro; IPR034466; Methyltransferase_Class_B.
DR InterPro; IPR007197; rSAM.
DR InterPro; IPR023404; rSAM_horseshoe.
DR PANTHER; PTHR43409; ANAEROBIC MAGNESIUM-PROTOPORPHYRIN IX MONOMETHYL ESTER CYCLASE-RELATED; 1.
DR PANTHER; PTHR43409:SF12; BLL1977 PROTEIN; 1.
DR Pfam; PF02310; B12-binding; 1.
DR Pfam; PF13282; DUF4070; 1.
DR Pfam; PF04055; Radical_SAM; 1.
DR SFLD; SFLDG01082; B12-binding_domain_containing; 1.
DR SFLD; SFLDF00415; ladderane_biosynthesis; 1.
DR SFLD; SFLDG01123; methyltransferase_(Class_B); 1.
DR SMART; SM00729; Elp3; 1.
DR SUPFAM; SSF102114; Radical SAM enzymes; 1.
DR PROSITE; PS51332; B12_BINDING; 1.
DR PROSITE; PS51918; RADICAL_SAM; 1.
PE 4: Predicted;
KW 4Fe-4S {ECO:0000256|ARBA:ARBA00022485};
KW Iron {ECO:0000256|ARBA:ARBA00022485};
KW Iron-sulfur {ECO:0000256|ARBA:ARBA00022485};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022485};
KW Reference proteome {ECO:0000313|Proteomes:UP000032309}.
FT DOMAIN 8..139
FT /note="B12-binding"
FT /evidence="ECO:0000259|PROSITE:PS51332"
FT DOMAIN 163..395
FT /note="Radical SAM core"
FT /evidence="ECO:0000259|PROSITE:PS51918"
SQ SEQUENCE 493 AA; 56758 MW; 4B59D800936EB95F CRC64;
MKRLVLINPH PIGNVGEENV SVLNQMPVNL GYLKVLTPKH WQVDIIDETQ ELAIDENTGE
ITFGGADLVG ITSVSYQAHR AYQIATACKK RGVPVIMGGI HATSYPEEAA KYVDCVVTRE
AVTTWEKIIG DFEKGCLQKR YDGVLTPMEL YQGLRPDRKY LKDKYKYRYS GIITTAGCPF
SCEFCSVPQF QGRKYRERPI EDVLDEFESI KGQYRGLILT DENFYGHSKK SNERVRDLFK
GMVDRGIYQN WFGFTSLNIY KDDETLEYMR KSGCVGVLIG IESINEEALK TMNKNVNLRI
SIENYFEAIA NIRKHGLAVW GTMVFGNDAD TPETFKQVAD FVLDSHVDIM TCGILCPFIN
TPLYHRLNGE GRLFRTDFPE DWKYYTSHHL TYVLKSMSLQ ELIDGFQYLY DRIYATEVLR
QRFQNAKEVL KDNMNAAMFA FRVNLDWQNV YQHLIQNLKE LQASGFYDEA LKRYNAQKKQ
GKKVELTPIE VSS
//
