ID A0A0D0Q6F3_9RHOB Unreviewed; 586 AA.
AC A0A0D0Q6F3;
DT 29-APR-2015, integrated into UniProtKB/TrEMBL.
DT 29-APR-2015, sequence version 1.
DT 20-DEC-2017, entry version 21.
DE RecName: Full=Malto-oligosyltrehalose trehalohydrolase {ECO:0000256|PIRNR:PIRNR006337};
DE Short=MTHase {ECO:0000256|PIRNR:PIRNR006337};
DE EC=3.2.1.141 {ECO:0000256|PIRNR:PIRNR006337};
DE AltName: Full=4-alpha-D-((1->4)-alpha-D-glucano)trehalose trehalohydrolase {ECO:0000256|PIRNR:PIRNR006337};
DE AltName: Full=Maltooligosyl trehalose trehalohydrolase {ECO:0000256|PIRNR:PIRNR006337};
GN ORFNames=Wenmar_01609 {ECO:0000313|EMBL:KIQ70039.1};
OS Wenxinia marina DSM 24838.
OC Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales;
OC Rhodobacteraceae; Wenxinia.
OX NCBI_TaxID=1123501 {ECO:0000313|EMBL:KIQ70039.1, ECO:0000313|Proteomes:UP000035100};
RN [1] {ECO:0000313|EMBL:KIQ70039.1, ECO:0000313|Proteomes:UP000035100}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 24838 {ECO:0000313|EMBL:KIQ70039.1,
RC ECO:0000313|Proteomes:UP000035100};
RA Fiebig A., Goeker M., Klenk H.-P.P.;
RL Submitted (JAN-2013) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY: Hydrolysis of (1->4)-alpha-D-glucosidic
CC linkage in 4-alpha-D-((1->4)-alpha-D-glucanosyl)(n) trehalose to
CC yield trehalose and (1->4)-alpha-D-glucan.
CC {ECO:0000256|PIRNR:PIRNR006337}.
CC -!- PATHWAY: Glycan biosynthesis; trehalose biosynthesis.
CC {ECO:0000256|PIRNR:PIRNR006337}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|PIRSR:PIRSR006337-1}.
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 13 family.
CC {ECO:0000256|PIRNR:PIRNR006337, ECO:0000256|SAAS:SAAS00964676}.
CC -!- CAUTION: The sequence shown here is derived from an
CC EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is
CC preliminary data. {ECO:0000313|EMBL:KIQ70039.1}.
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DR EMBL; AONG01000008; KIQ70039.1; -; Genomic_DNA.
DR RefSeq; WP_018301029.1; NZ_KN848372.1.
DR EnsemblBacteria; KIQ70039; KIQ70039; Wenmar_01609.
DR PATRIC; fig|1123501.6.peg.1702; -.
DR UniPathway; UPA00299; -.
DR Proteomes; UP000035100; Unassembled WGS sequence.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0033942; F:4-alpha-D-(1->4)-alpha-D-glucanotrehalose trehalohydrolase activity; IEA:UniProtKB-EC.
DR GO; GO:0005992; P:trehalose biosynthetic process; IEA:UniProtKB-UniPathway.
DR Gene3D; 2.60.40.10; -; 1.
DR InterPro; IPR022567; DUF3459.
DR InterPro; IPR006047; Glyco_hydro_13_cat_dom.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR InterPro; IPR013783; Ig-like_fold.
DR InterPro; IPR014756; Ig_E-set.
DR InterPro; IPR012768; Trehalose_TreZ.
DR Pfam; PF00128; Alpha-amylase; 2.
DR Pfam; PF11941; DUF3459; 1.
DR PIRSF; PIRSF006337; Trehalose_TreZ; 1.
DR SMART; SM00642; Aamy; 1.
DR SUPFAM; SSF51445; SSF51445; 1.
DR SUPFAM; SSF81296; SSF81296; 1.
DR TIGRFAMs; TIGR02402; trehalose_TreZ; 1.
PE 3: Inferred from homology;
KW Complete proteome {ECO:0000313|Proteomes:UP000035100};
KW Glycosidase {ECO:0000256|PIRNR:PIRNR006337,
KW ECO:0000313|EMBL:KIQ70039.1};
KW Hydrolase {ECO:0000256|PIRNR:PIRNR006337,
KW ECO:0000313|EMBL:KIQ70039.1};
KW Reference proteome {ECO:0000313|Proteomes:UP000035100}.
FT DOMAIN 63 453 Aamy. {ECO:0000259|SMART:SM00642}.
FT ACT_SITE 257 257 Nucleophile. {ECO:0000256|PIRSR:
FT PIRSR006337-1}.
FT ACT_SITE 292 292 Proton donor. {ECO:0000256|PIRSR:
FT PIRSR006337-1}.
FT SITE 387 387 Transition state stabilizer.
FT {ECO:0000256|PIRSR:PIRSR006337-3}.
SQ SEQUENCE 586 AA; 64717 MW; 428D71ED9C4B328A CRC64;
MTDRRPAWGA VPTRPGHWHL RLWAPGADSL SLRLGAADRP MEAEGDGWFG LEVEAEEGAP
YSFVLPDGMA VPDPAARRQQ GDVHGPSLLT APPADDPWAG WSGRPWEEAA ILELHVGTFT
GEGTFRAAID RLDHIAKAGF TAIEIMPVAQ FAGDRGWGYD GVLLYAPHPA YGTPDDLRAL
VRAAHERGLM VLLDVVYNHF GPEGSYLHAY APDFFHDEKD TPWGGAIAFE RQPVRRFMIE
NGLYWLREFG FDGLRLDAID HIDDPSDEEV LIEFARELRA ALPGRPVHLT TEDSRNVTHL
HEREDGRVTL HTAEWNDDFH NVAHVIATGE TEAYYADFAQ KEWAHLARTL AEGFAYQGEP
DRSGKVRGKT SGHQPPTAFV DFLQNHDQIG NRAFGERLCN LASPRMVDAL TAILLLSPHI
PLMFMGEEYG ETRSFCFFAG FDGDLARAVT EGRRREFADF SAFTVADTSS IPDPIARSTF
EASKLDWAKL SDDDHRATLD RTRHLLTLRR ERIVPLLAGA GPHCGTVLKA DEGAIAVDWR
LGGGALLQLR ANLEDQPRDL PPATGEELHR VGAPGGPISA VHWLGT
//
