ID   A0A0D2GN46_9EURO        Unreviewed;       648 AA.
AC   A0A0D2GN46;
DT   29-APR-2015, integrated into UniProtKB/TrEMBL.
DT   29-APR-2015, sequence version 1.
DT   27-MAR-2024, entry version 29.
DE   SubName: Full=tRNA pseudouridine(38-40) synthase, variant {ECO:0000313|EMBL:KIW73819.1};
GN   ORFNames=PV04_01907 {ECO:0000313|EMBL:KIW73819.1};
OS   Phialophora macrospora.
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC   Chaetothyriomycetidae; Chaetothyriales; Herpotrichiellaceae; Phialophora.
OX   NCBI_TaxID=1851006 {ECO:0000313|EMBL:KIW73819.1, ECO:0000313|Proteomes:UP000054266};
RN   [1] {ECO:0000313|EMBL:KIW73819.1, ECO:0000313|Proteomes:UP000054266}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CBS 27337 {ECO:0000313|EMBL:KIW73819.1,
RC   ECO:0000313|Proteomes:UP000054266};
RG   The Broad Institute Genomics Platform;
RA   Cuomo C., de Hoog S., Gorbushina A., Stielow B., Teixiera M.,
RA   Abouelleil A., Chapman S.B., Priest M., Young S.K., Wortman J., Nusbaum C.,
RA   Birren B.;
RT   "The Genome Sequence of Capronia semiimmersa CBS27337.";
RL   Submitted (JAN-2015) to the EMBL/GenBank/DDBJ databases.
CC   -!- SIMILARITY: Belongs to the tRNA pseudouridine synthase TruA family.
CC       {ECO:0000256|ARBA:ARBA00009375}.
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DR   EMBL; KN846956; KIW73819.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A0D2GN46; -.
DR   HOGENOM; CLU_021971_1_0_1; -.
DR   OrthoDB; 12038at2759; -.
DR   Proteomes; UP000054266; Unassembled WGS sequence.
DR   GO; GO:0043231; C:intracellular membrane-bounded organelle; IEA:UniProt.
DR   GO; GO:0009982; F:pseudouridine synthase activity; IEA:InterPro.
DR   GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR   GO; GO:0031119; P:tRNA pseudouridine synthesis; IEA:InterPro.
DR   CDD; cd02568; PseudoU_synth_PUS1_PUS2; 1.
DR   Gene3D; 3.30.70.660; Pseudouridine synthase I, catalytic domain, C-terminal subdomain; 1.
DR   Gene3D; 3.30.70.580; Pseudouridine synthase I, catalytic domain, N-terminal subdomain; 1.
DR   InterPro; IPR020103; PsdUridine_synth_cat_dom_sf.
DR   InterPro; IPR001406; PsdUridine_synth_TruA.
DR   InterPro; IPR020097; PsdUridine_synth_TruA_a/b_dom.
DR   InterPro; IPR020095; PsdUridine_synth_TruA_C.
DR   InterPro; IPR041708; PUS1/PUS2-like.
DR   InterPro; IPR020094; TruA/RsuA/RluB/E/F_N.
DR   PANTHER; PTHR11142; PSEUDOURIDYLATE SYNTHASE; 1.
DR   PANTHER; PTHR11142:SF4; PSEUDOURIDYLATE SYNTHASE 1 HOMOLOG; 1.
DR   Pfam; PF01416; PseudoU_synth_1; 1.
DR   SUPFAM; SSF55120; Pseudouridine synthase; 1.
PE   3: Inferred from homology;
KW   Isomerase {ECO:0000256|ARBA:ARBA00023235};
KW   tRNA processing {ECO:0000256|ARBA:ARBA00022694}.
FT   DOMAIN          420..527
FT                   /note="Pseudouridine synthase I TruA alpha/beta"
FT                   /evidence="ECO:0000259|Pfam:PF01416"
FT   REGION          19..46
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          361..386
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          623..648
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        370..385
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   ACT_SITE        195
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR641708-1"
FT   BINDING         251
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR641708-2"
SQ   SEQUENCE   648 AA;  73711 MW;  994536795487F3F8 CRC64;
     MLYQSPIWRL AFRRPPRQVR PRPLRPLMGT RDSIPHPSVK DTERPRKFAN KDDVIQMQDV
     RVRMIFRGEE AEVVEEVVRA RREIWGGRSG LDKRQRNEDE QAAKRQRLDK GVSELPIYAT
     KFSDDVLAAE ERRPKKKVAV LIGYAGTGYK GMQLTHDQKT IEGDLFQAFV AAGAISKANA
     DDPKKSSFVR CARTDKGVHA AGNVISLKLI VEDEDVVQKI NENLAPQIRV WGYERTNNAF
     SAYQLVDSRI YEYLIPTHSF LPPHPTSFLG RNCDKWAEKK GDQDEYRKRQ EEVLGYWEKV
     DEEMIKPILA EYDPETRDIL EKALWLKGND PSGSEAAAEI VESELEPKMA ETASADHIEI
     KDDIAQDDEP TKTSSGTTAN PRTSRRQILD EAIKRLRQAY ISAKRAYRIP PGRLERIQKA
     LNLFVGTRNY HNYTVQKTYK DPSAKRVIKS FAVNKTPILI NGTEWLSMKV HGQSFMMHQI
     RKMVGMVALV VRCGCDPNRL LDSMGPARLS IPKAPSLGLL LERPVFDSYN KRATDSLGKL
     PIDFDKFKDE MDRFKQEQIY ERMYRDEEKE NVFGNFFNHV DNFPSETFLF VTSGGVEATM
     TKPLVGAVEN GQDDKNLEAA LTEKGANVDA EDGDSDVDDV KDEHGNEG
//