ID   A0A0D2VZI8_CAPO3        Unreviewed;       408 AA.
AC   A0A0D2VZI8;
DT   29-APR-2015, integrated into UniProtKB/TrEMBL.
DT   29-APR-2015, sequence version 1.
DT   28-JUN-2023, entry version 24.
DE   RecName: Full=tRNA pseudouridine synthase {ECO:0000256|RuleBase:RU003792};
DE            EC=5.4.99.12 {ECO:0000256|RuleBase:RU003792};
GN   ORFNames=CAOG_007708 {ECO:0000313|EMBL:KJE97272.1};
OS   Capsaspora owczarzaki (strain ATCC 30864).
OC   Eukaryota; Filasterea; Capsaspora.
OX   NCBI_TaxID=595528 {ECO:0000313|EMBL:KJE97272.1, ECO:0000313|Proteomes:UP000008743};
RN   [1] {ECO:0000313|Proteomes:UP000008743}
RP   NUCLEOTIDE SEQUENCE.
RC   STRAIN=ATCC 30864 {ECO:0000313|Proteomes:UP000008743};
RA   Russ C., Cuomo C., Burger G., Gray M.W., Holland P.W.H., King N.,
RA   Lang F.B.F., Roger A.J., Ruiz-Trillo I., Young S.K., Zeng Q., Gargeya S.,
RA   Alvarado L., Berlin A., Chapman S.B., Chen Z., Freedman E., Gellesch M.,
RA   Goldberg J., Griggs A., Gujja S., Heilman E., Heiman D., Howarth C.,
RA   Mehta T., Neiman D., Pearson M., Roberts A., Saif S., Shea T., Shenoy N.,
RA   Sisk P., Stolte C., Sykes S., White J., Yandava C., Haas B., Nusbaum C.,
RA   Birren B.;
RT   "The Genome Sequence of Capsaspora owczarzaki ATCC 30864.";
RL   Submitted (FEB-2011) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=uridine(38/39/40) in tRNA = pseudouridine(38/39/40) in tRNA;
CC         Xref=Rhea:RHEA:22376, Rhea:RHEA-COMP:10085, Rhea:RHEA-COMP:10087,
CC         ChEBI:CHEBI:65314, ChEBI:CHEBI:65315; EC=5.4.99.12;
CC         Evidence={ECO:0000256|RuleBase:RU003792};
CC   -!- SIMILARITY: Belongs to the tRNA pseudouridine synthase TruA family.
CC       {ECO:0000256|ARBA:ARBA00009375, ECO:0000256|RuleBase:RU003792}.
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DR   EMBL; KE346373; KJE97272.1; -; Genomic_DNA.
DR   RefSeq; XP_004343582.2; XM_004343532.2.
DR   AlphaFoldDB; A0A0D2VZI8; -.
DR   STRING; 595528.A0A0D2VZI8; -.
DR   EnsemblProtists; KJE97272; KJE97272; CAOG_007708.
DR   GeneID; 14895557; -.
DR   eggNOG; KOG4393; Eukaryota.
DR   InParanoid; A0A0D2VZI8; -.
DR   OrthoDB; 120142at2759; -.
DR   PhylomeDB; A0A0D2VZI8; -.
DR   Proteomes; UP000008743; Unassembled WGS sequence.
DR   GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR   GO; GO:0106029; F:tRNA pseudouridine synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0001522; P:pseudouridine synthesis; IEA:InterPro.
DR   GO; GO:0008033; P:tRNA processing; IEA:UniProtKB-KW.
DR   Gene3D; 3.30.70.660; Pseudouridine synthase I, catalytic domain, C-terminal subdomain; 1.
DR   Gene3D; 3.30.70.580; Pseudouridine synthase I, catalytic domain, N-terminal subdomain; 1.
DR   HAMAP; MF_00171; TruA; 1.
DR   InterPro; IPR020103; PsdUridine_synth_cat_dom_sf.
DR   InterPro; IPR001406; PsdUridine_synth_TruA.
DR   InterPro; IPR020097; PsdUridine_synth_TruA_a/b_dom.
DR   InterPro; IPR020095; PsdUridine_synth_TruA_C.
DR   InterPro; IPR020094; TruA/RsuA/RluB/E/F_N.
DR   PANTHER; PTHR11142; PSEUDOURIDYLATE SYNTHASE; 1.
DR   PANTHER; PTHR11142:SF0; TRNA PSEUDOURIDINE SYNTHASE-LIKE 1; 1.
DR   Pfam; PF01416; PseudoU_synth_1; 2.
DR   SUPFAM; SSF55120; Pseudouridine synthase; 1.
PE   3: Inferred from homology;
KW   Isomerase {ECO:0000256|ARBA:ARBA00023235, ECO:0000256|RuleBase:RU003792};
KW   Reference proteome {ECO:0000313|Proteomes:UP000008743};
KW   tRNA processing {ECO:0000256|ARBA:ARBA00022694,
KW   ECO:0000256|RuleBase:RU003792}.
FT   DOMAIN          83..199
FT                   /note="Pseudouridine synthase I TruA alpha/beta"
FT                   /evidence="ECO:0000259|Pfam:PF01416"
FT   DOMAIN          239..361
FT                   /note="Pseudouridine synthase I TruA alpha/beta"
FT                   /evidence="ECO:0000259|Pfam:PF01416"
SQ   SEQUENCE   408 AA;  45045 MW;  D8196A2D42A6D327 CRC64;
     MAHVAVFRGL HVHHTQLNHA RWVSLASSLS STLTGIDQRR SFSTVKDSSI AEVATAKTTT
     ATTAEMQLDG QQPPVSASRF LLAVEYDGTA FSGVAASPDE PPDRPSVGSV LDRAIAQATR
     QDARVTFSSR TDAGVHALWN TCHVDIEPRT RRGDLMTQQQ WTPLALGSAI NYFSRGKVPY
     PMSVRAVCAV PEQVHARFNA TQRVYIYRVV VGESSAASVF DLRYHLTAAP IDESLVQQAC
     QVFEGTHDFQ SFRAARCQAR TTTRHISQFS VQIDKHKTDN PWANSLPDAL SKPTVALTFT
     IRGSGFLYHQ VRIMVGHVLS VASGKSTLAD LVHLRDNPQV IHLHKDMLPA HGLCLKEVCY
     DSQDIPPESW VMIAKKHHSF KALRESVIVR KPFRNPAKPE AAVVDQQD
//