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Database: UniProt
Entry: A0A0D3AT97_BRAOL
LinkDB: A0A0D3AT97_BRAOL
Original site: A0A0D3AT97_BRAOL 
ID   A0A0D3AT97_BRAOL        Unreviewed;       913 AA.
AC   A0A0D3AT97;
DT   29-APR-2015, integrated into UniProtKB/TrEMBL.
DT   29-APR-2015, sequence version 1.
DT   22-NOV-2017, entry version 15.
DE   RecName: Full=Non-lysosomal glucosylceramidase {ECO:0000256|PIRNR:PIRNR028944};
DE            Short=NLGase {ECO:0000256|PIRNR:PIRNR028944};
DE            EC=3.2.1.45 {ECO:0000256|PIRNR:PIRNR028944};
GN   Name=106327092 {ECO:0000313|EnsemblPlants:Bo2g110640.1};
OS   Brassica oleracea var. oleracea.
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliophyta; eudicotyledons; Gunneridae;
OC   Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Brassiceae;
OC   Brassica.
OX   NCBI_TaxID=109376 {ECO:0000313|EnsemblPlants:Bo2g110640.1, ECO:0000313|Proteomes:UP000032141};
RN   [1] {ECO:0000313|EnsemblPlants:Bo2g110640.1, ECO:0000313|Proteomes:UP000032141}
RP   NUCLEOTIDE SEQUENCE.
RC   STRAIN=cv. TO1000 {ECO:0000313|Proteomes:UP000032141};
RX   PubMed=24916971; DOI=10.1186/gb-2014-15-6-r77;
RA   Parkin I.A., Koh C., Tang H., Robinson S.J., Kagale S., Clarke W.E.,
RA   Town C.D., Nixon J., Krishnakumar V., Bidwell S.L., Denoeud F.,
RA   Belcram H., Links M.G., Just J., Clarke C., Bender T., Huebert T.,
RA   Mason A.S., Pires J.C., Barker G., Moore J., Walley P.G., Manoli S.,
RA   Batley J., Edwards D., Nelson M.N., Wang X., Paterson A.H., King G.,
RA   Bancroft I., Chalhoub B., Sharpe A.G.;
RT   "Transcriptome and methylome profiling reveals relics of genome
RT   dominance in the mesopolyploid Brassica oleracea.";
RL   Genome Biol. 15:R77-R77(2014).
RN   [2] {ECO:0000313|EnsemblPlants:Bo2g110640.1}
RP   IDENTIFICATION.
RG   EnsemblPlants;
RL   Submitted (MAR-2015) to UniProtKB.
CC   -!- FUNCTION: Non-lysosomal glucosylceramidase that catalyzes the
CC       conversion of glucosylceramide to free glucose and ceramide.
CC       {ECO:0000256|PIRNR:PIRNR028944}.
CC   -!- CATALYTIC ACTIVITY: D-glucosyl-N-acylsphingosine + H(2)O = D-
CC       glucose + N-acylsphingosine. {ECO:0000256|PIRNR:PIRNR028944}.
CC   -!- SIMILARITY: Belongs to the non-lysosomal glucosylceramidase
CC       family. {ECO:0000256|PIRNR:PIRNR028944}.
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DR   RefSeq; XP_013620570.1; XM_013765116.1.
DR   EnsemblPlants; Bo2g110640.1; Bo2g110640.1; Bo2g110640.
DR   GeneID; 106327092; -.
DR   Gramene; Bo2g110640.1; Bo2g110640.1; Bo2g110640.
DR   KEGG; boe:106327092; -.
DR   KO; K17108; -.
DR   OMA; NERTYHR; -.
DR   Proteomes; UP000032141; Chromosome C2.
DR   GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005773; C:vacuole; IEA:EnsemblPlants.
DR   GO; GO:0004348; F:glucosylceramidase activity; IEA:UniProtKB-EC.
DR   GO; GO:0006680; P:glucosylceramide catabolic process; IEA:InterPro.
DR   InterPro; IPR008928; 6-hairpin_glycosidase-like.
DR   InterPro; IPR014551; B_Glucosidase_GBA2-typ.
DR   InterPro; IPR006775; GH116_catalytic.
DR   InterPro; IPR024462; GH116_N.
DR   Pfam; PF04685; DUF608; 1.
DR   Pfam; PF12215; Glyco_hydr_116N; 1.
DR   PIRSF; PIRSF028944; Beta_gluc_GBA2; 1.
DR   SUPFAM; SSF48208; SSF48208; 1.
PE   3: Inferred from homology;
KW   Complete proteome {ECO:0000313|Proteomes:UP000032141};
KW   Glycosidase {ECO:0000256|PIRNR:PIRNR028944};
KW   Hydrolase {ECO:0000256|PIRNR:PIRNR028944};
KW   Lipid metabolism {ECO:0000256|PIRNR:PIRNR028944};
KW   Membrane {ECO:0000256|PIRNR:PIRNR028944};
KW   Reference proteome {ECO:0000313|Proteomes:UP000032141}.
FT   DOMAIN       82    399       Glyco_hydr_116N. {ECO:0000259|Pfam:
FT                                PF12215}.
FT   DOMAIN      486    846       DUF608. {ECO:0000259|Pfam:PF04685}.
SQ   SEQUENCE   913 AA;  102663 MW;  E66B8B4318AE8721 CRC64;
     MESELQTQLV GSETLSLPQV TWQRKLNSKV KNPSEFKMST SDFLHLFPIG YRLWRHTKQE
     AAKGKVSIYD IFKKKNVKGN HGVPLGGVGA GSIGRSYKGE FQQFKLFPKV CEEAPILTNQ
     FSVFVSRPGG VNYSTVLCAT SPESANGRTE DLGIESWDWK IKGDKSTYHA LYPRSWTVFN
     EPDPKLRIVS RQVSPFIPNN YKESSFPVSV FAFTATNLGK EEATVTLLFT WENSVGGASG
     FTGEHFNSSI MERDGVHAIA LHHKTANGHP PVTYAIAAQE TDDVSVSECP CFLVSGYSPN
     EITPRAMWDE IKKNKSFDQL PCEPGSPSRP GTAIGAAIAA KVKVPPGCDR TVTFSLSWDC
     PEVRFNERTY HRRYTRFYGS LGDAAVNMAR DALLNYGNWE SQIEEWQNTI LLDTSLPDWY
     RVTLFNELYY FNSGGAIWTD GLPYKQTTER STEQNSNDTD ILQKINAVCD QVHHSPQSQN
     AEENIGQFLY LEGIEYLMYN TYDVHFYSSF PLLILFSKLE LSIQRDFAAA VMFQDPTKKE
     IMSSGELVPR KLLGSVPHDI GLNNPWLELN AYNLFNTDRW KDLNAKFVLQ VYRDVVATGD
     YSFARAVWPS VYLAVAYLDQ FDKDEDGMIE NEGLPDQTYD TWSVTGVSAY CGGLYVAALQ
     AASAFATIVG ENVVAFYFNA KYEKAKTVYE KLWNGSYFDY DNSGSDSSSS ILADQLAGQW
     YARACGLKPI TKEEWIKKAL ETIHEFNVMK VRGGTRGAVN GMSPDGQVDT NSLVSKEVWA
     GTTYSVAACM IQEGQREKGF QTASGIYEAV WSDRGLSCSF QTPEAWNMND EYRSLCYMRP
     LAIWAIQWAL TPIQTFGGEK QNLMAGDEDE SDLLLRQHNG FKEVARYVKI AKSTEHRSLL
     QEKYEAILKT FHL
//
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